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Regulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.

Engagement of high-affinity immunoglobulin E receptors (Fc[epsilon]RI) activates two signaling pathways in mast cells. The Lyn pathway leads to recruitment of Syk and to calcium mobilization whereas the Fyn pathway leads to phosphatidylinositol 3-kinase recruitment. Mapping the connections between b... Full description

Journal Title: PloS one 2014, Vol.9(10), p.e109800
Main Author: Kassas, Asma
Other Authors: Moura, Ivan C , Yamashita, Yumi , Scheffel, Jorg , Guérin-Marchand, Claudine , Blank, Ulrich , Sims, Peter J , Wiedmer, Therese , Monteiro, Renato C , Rivera, Juan , Charles, Nicolas , Benhamou, Marc
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109800
Link: http://search.proquest.com/docview/1609505767/?pq-origsite=primo
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title: Regulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
format: Article
creator:
  • Kassas, Asma
  • Moura, Ivan C
  • Yamashita, Yumi
  • Scheffel, Jorg
  • Guérin-Marchand, Claudine
  • Blank, Ulrich
  • Sims, Peter J
  • Wiedmer, Therese
  • Monteiro, Renato C
  • Rivera, Juan
  • Charles, Nicolas
  • Benhamou, Marc
subjects:
  • Animals–Metabolism
  • Calcium–Immunology
  • Cell Degranulation–Genetics
  • Cell Line–Immunology
  • Gene Expression Regulation–Cytology
  • Intracellular Signaling Peptides and Proteins–Immunology
  • Mast Cells–Genetics
  • Mice–Immunology
  • Phosphatidylinositol 3-Kinases–Genetics
  • Phospholipid Transfer Proteins–Immunology
  • Phosphorylation–Genetics
  • Protein-Tyrosine Kinases–Immunology
  • Proto-Oncogene Proteins C-Fyn–Genetics
  • Rats–Immunology
  • Receptors, Ige–Genetics
  • Signal Transduction–Immunology
  • Syk Kinase–Metabolism
  • Tyrosine–Genetics
  • Src-Family Kinases–Immunology
  • Fc-Epsilon Receptor I Beta-Chain, Mouse
  • Intracellular Signaling Peptides and Proteins
  • Phospholipid Transfer Proteins
  • Plscr1 Protein, Mouse
  • Receptors, Ige
  • Tyrosine
  • Phosphatidylinositol 3-Kinases
  • Protein-Tyrosine Kinases
  • Fyn Protein, Mouse
  • Proto-Oncogene Proteins C-Fyn
  • Syk Kinase
  • Syk Protein, Mouse
  • Syk Protein, Rat
  • Lyn Protein-Tyrosine Kinase
  • Src-Family Kinases
  • Calcium
ispartof: PloS one, 2014, Vol.9(10), p.e109800
description: Engagement of high-affinity immunoglobulin E receptors (Fc[epsilon]RI) activates two signaling pathways in mast cells. The Lyn pathway leads to recruitment of Syk and to calcium mobilization whereas the Fyn pathway leads to phosphatidylinositol 3-kinase recruitment. Mapping the connections between both pathways remains an important task to be completed. We previously reported that Phospholipid Scramblase 1 (PLSCR1) is phosphorylated on tyrosine after cross-linking Fc[epsilon]RI on RBL-2H3 rat mast cells, amplifies mast cell degranulation, and is associated with both Lyn and Syk tyrosine kinases. Here, analysis of the pathway leading to PLSCR1 tyrosine phosphorylation reveals that it depends on the FcR[gamma] chain. Fc[epsilon]RI aggregation in Fyn-deficient mouse bone marrow-derived mast cells (BMMC) induced a more robust increase in Fc[epsilon]RI-dependent tyrosine phosphorylation of PLSCR1 compared to wild-type cells, whereas PLSCR1 phosphorylation was abolished in Lyn-deficient BMMC. Lyn association with PLSCR1 was not altered in Fyn-deficient BMMC. PLSCR1 phosphorylation was also dependent on the kinase Syk and significantly, but partially, dependent on detectable calcium mobilization. Thus, the Lyn/Syk/calcium axis promotes PLSCR1 phosphorylation in multiple ways. Conversely, the Fyn-dependent pathway negatively regulates it. This study reveals a complex regulation for PLSCR1 tyrosine phosphorylation in Fc[epsilon]RI-activated mast cells and that PLSCR1 sits at a crossroads between Lyn and Fyn pathways.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109800
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleRegulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
creatorKassas, Asma ; Moura, Ivan C ; Yamashita, Yumi ; Scheffel, Jorg ; Guérin-Marchand, Claudine ; Blank, Ulrich ; Sims, Peter J ; Wiedmer, Therese ; Monteiro, Renato C ; Rivera, Juan ; Charles, Nicolas ; Benhamou, Marc
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ispartofPloS one, 2014, Vol.9(10), p.e109800
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109800
subjectAnimals–Metabolism ; Calcium–Immunology ; Cell Degranulation–Genetics ; Cell Line–Immunology ; Gene Expression Regulation–Cytology ; Intracellular Signaling Peptides and Proteins–Immunology ; Mast Cells–Genetics ; Mice–Immunology ; Phosphatidylinositol 3-Kinases–Genetics ; Phospholipid Transfer Proteins–Immunology ; Phosphorylation–Genetics ; Protein-Tyrosine Kinases–Immunology ; Proto-Oncogene Proteins C-Fyn–Genetics ; Rats–Immunology ; Receptors, Ige–Genetics ; Signal Transduction–Immunology ; Syk Kinase–Metabolism ; Tyrosine–Genetics ; Src-Family Kinases–Immunology ; Fc-Epsilon Receptor I Beta-Chain, Mouse ; Intracellular Signaling Peptides and Proteins ; Phospholipid Transfer Proteins ; Plscr1 Protein, Mouse ; Receptors, Ige ; Tyrosine ; Phosphatidylinositol 3-Kinases ; Protein-Tyrosine Kinases ; Fyn Protein, Mouse ; Proto-Oncogene Proteins C-Fyn ; Syk Kinase ; Syk Protein, Mouse ; Syk Protein, Rat ; Lyn Protein-Tyrosine Kinase ; Src-Family Kinases ; Calcium
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descriptionEngagement of high-affinity immunoglobulin E receptors (Fc[epsilon]RI) activates two signaling pathways in mast cells. The Lyn pathway leads to recruitment of Syk and to calcium mobilization whereas the Fyn pathway leads to phosphatidylinositol 3-kinase recruitment. Mapping the connections between both pathways remains an important task to be completed. We previously reported that Phospholipid Scramblase 1 (PLSCR1) is phosphorylated on tyrosine after cross-linking Fc[epsilon]RI on RBL-2H3 rat mast cells, amplifies mast cell degranulation, and is associated with both Lyn and Syk tyrosine kinases. Here, analysis of the pathway leading to PLSCR1 tyrosine phosphorylation reveals that it depends on the FcR[gamma] chain. Fc[epsilon]RI aggregation in Fyn-deficient mouse bone marrow-derived mast cells (BMMC) induced a more robust increase in Fc[epsilon]RI-dependent tyrosine phosphorylation of PLSCR1 compared to wild-type cells, whereas PLSCR1 phosphorylation was abolished in Lyn-deficient BMMC. Lyn association with PLSCR1 was not altered in Fyn-deficient BMMC. PLSCR1 phosphorylation was also dependent on the kinase Syk and significantly, but partially, dependent on detectable calcium mobilization. Thus, the Lyn/Syk/calcium axis promotes PLSCR1 phosphorylation in multiple ways. Conversely, the Fyn-dependent pathway negatively regulates it. This study reveals a complex regulation for PLSCR1 tyrosine phosphorylation in Fc[epsilon]RI-activated mast cells and that PLSCR1 sits at a crossroads between Lyn and Fyn pathways.
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titleRegulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
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titleRegulation of the tyrosine phosphorylation of Phospholipid Scramblase 1 in mast cells that are stimulated through the high-affinity IgE receptor.
authorKassas, Asma ; Moura, Ivan C ; Yamashita, Yumi ; Scheffel, Jorg ; Guérin-Marchand, Claudine ; Blank, Ulrich ; Sims, Peter J ; Wiedmer, Therese ; Monteiro, Renato C ; Rivera, Juan ; Charles, Nicolas ; Benhamou, Marc
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7Mice–Immunology
8Phosphatidylinositol 3-Kinases–Genetics
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