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Scorpion venom heat-resistant peptide (SVHRP) enhances neurogenesis and neurite outgrowth of immature neurons in adult mice by up-regulating brain-derived neurotrophic factor (BDNF).

Scorpion venom heat-resistant peptide (SVHRP) is a component purified from Buthus martensii Karsch scorpion venom. Although scorpions and their venom have been used in Traditional Chinese Medicine (TCM) to treat chronic neurological disorders, the underlying mechanisms of these treatments remain unk... Full description

Journal Title: PloS one 2014, Vol.9(10), p.e109977
Main Author: Wang, Tao
Other Authors: Wang, Shi-Wei , Zhang, Yue , Wu, Xue-Fei , Peng, Yan , Cao, Zhen , Ge, Bi-Ying , Wang, Xi , Wu, Qiong , Lin, Jin-Tao , Zhang, Wan-Qin , Li, Shao , Zhao, Jie
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109977
Link: http://search.proquest.com/docview/1610755316/?pq-origsite=primo
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title: Scorpion venom heat-resistant peptide (SVHRP) enhances neurogenesis and neurite outgrowth of immature neurons in adult mice by up-regulating brain-derived neurotrophic factor (BDNF).
format: Article
creator:
  • Wang, Tao
  • Wang, Shi-Wei
  • Zhang, Yue
  • Wu, Xue-Fei
  • Peng, Yan
  • Cao, Zhen
  • Ge, Bi-Ying
  • Wang, Xi
  • Wu, Qiong
  • Lin, Jin-Tao
  • Zhang, Wan-Qin
  • Li, Shao
  • Zhao, Jie
subjects:
  • Animals–Drug Effects
  • Astrocytes–Antagonists & Inhibitors
  • Brain-Derived Neurotrophic Factor–Biosynthesis
  • Cell Proliferation–Chemistry
  • Culture Media, Conditioned–Drug Effects
  • Gene Expression Regulation, Developmental–Antagonists & Inhibitors
  • Glial Fibrillary Acidic Protein–Biosynthesis
  • Humans–Drug Effects
  • Mice–Drug Effects
  • Nerve Tissue Proteins–Drug Effects
  • Neurites–Administration & Dosage
  • Neurogenesis–Administration & Dosage
  • Neurons–Administration & Dosage
  • Peptides–Administration & Dosage
ispartof: PloS one, 2014, Vol.9(10), p.e109977
description: Scorpion venom heat-resistant peptide (SVHRP) is a component purified from Buthus martensii Karsch scorpion venom. Although scorpions and their venom have been used in Traditional Chinese Medicine (TCM) to treat chronic neurological disorders, the underlying mechanisms of these treatments remain unknown. We applied SVHRP in vitro and in vivo to understand its effects on the neurogenesis and maturation of adult immature neurons and explore associated molecular mechanisms. SVHRP administration increased the number of 5-bromo-2'-dexoxyuridine (BrdU)-positive cells, BrdU- positive/neuron-specific nuclear protein (NeuN)-positive neurons, and polysialylated-neural cell adhesion molecule (PSA-NCAM)-positive immature neurons in the subventricular zone (SVZ) and subgranular zone (SGZ) of hippocampus. Furthermore immature neurons incubated with SVHRP-pretreated astrocyte-conditioned medium exhibited significantly increased neurite length compared with those incubated with normal astrocyte-conditioned medium. This neurotrophic effect was further confirmed in vivo by detecting an increased average single area and whole area of immature neurons in the SGZ, SVZ and olfactory bulb (OB) in the adult mouse brain. In contrast to normal astrocyte-conditioned medium, higher concentrations of brain-derived neurotrophic factor (BDNF) but not nerve growth factor (NGF) or glial cell line-derived neurotrophic factor (GDNF) was detected in the conditioned medium of SVHRP-pretreated astrocytes, and blocking BDNF using anti-BDNF antibodies eliminated these SVHRP-dependent neurotrophic effects. In SVHRP treated mouse brain, more glial fibrillary acidic protein (GFAP)-positive cells were detected. Furthermore, immunohistochemistry revealed increased numbers of GFAP/BDNF double-positive cells, which agrees with the observed changes in the culture system. This paper describes novel effects of scorpion venom-originated peptide on the stem cells and suggests the potential therapeutic values of SVHRP.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0109977
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleScorpion venom heat-resistant peptide (SVHRP) enhances neurogenesis and neurite outgrowth of immature neurons in adult mice by up-regulating brain-derived neurotrophic factor (BDNF).
creatorWang, Tao ; Wang, Shi-Wei ; Zhang, Yue ; Wu, Xue-Fei ; Peng, Yan ; Cao, Zhen ; Ge, Bi-Ying ; Wang, Xi ; Wu, Qiong ; Lin, Jin-Tao ; Zhang, Wan-Qin ; Li, Shao ; Zhao, Jie
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descriptionScorpion venom heat-resistant peptide (SVHRP) is a component purified from Buthus martensii Karsch scorpion venom. Although scorpions and their venom have been used in Traditional Chinese Medicine (TCM) to treat chronic neurological disorders, the underlying mechanisms of these treatments remain unknown. We applied SVHRP in vitro and in vivo to understand its effects on the neurogenesis and maturation of adult immature neurons and explore associated molecular mechanisms. SVHRP administration increased the number of 5-bromo-2'-dexoxyuridine (BrdU)-positive cells, BrdU- positive/neuron-specific nuclear protein (NeuN)-positive neurons, and polysialylated-neural cell adhesion molecule (PSA-NCAM)-positive immature neurons in the subventricular zone (SVZ) and subgranular zone (SGZ) of hippocampus. Furthermore immature neurons incubated with SVHRP-pretreated astrocyte-conditioned medium exhibited significantly increased neurite length compared with those incubated with normal astrocyte-conditioned medium. This neurotrophic effect was further confirmed in vivo by detecting an increased average single area and whole area of immature neurons in the SGZ, SVZ and olfactory bulb (OB) in the adult mouse brain. In contrast to normal astrocyte-conditioned medium, higher concentrations of brain-derived neurotrophic factor (BDNF) but not nerve growth factor (NGF) or glial cell line-derived neurotrophic factor (GDNF) was detected in the conditioned medium of SVHRP-pretreated astrocytes, and blocking BDNF using anti-BDNF antibodies eliminated these SVHRP-dependent neurotrophic effects. In SVHRP treated mouse brain, more glial fibrillary acidic protein (GFAP)-positive cells were detected. Furthermore, immunohistochemistry revealed increased numbers of GFAP/BDNF double-positive cells, which agrees with the observed changes in the culture system. This paper describes novel effects of scorpion venom-originated peptide on the stem cells and suggests the potential therapeutic values of SVHRP.
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titleScorpion venom heat-resistant peptide (SVHRP) enhances neurogenesis and neurite outgrowth of immature neurons in adult mice by up-regulating brain-derived neurotrophic factor (BDNF).
authorWang, Tao ; Wang, Shi-Wei ; Zhang, Yue ; Wu, Xue-Fei ; Peng, Yan ; Cao, Zhen ; Ge, Bi-Ying ; Wang, Xi ; Wu, Qiong ; Lin, Jin-Tao ; Zhang, Wan-Qin ; Li, Shao ; Zhao, Jie
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