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Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure.

The hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isofo... Full description

Journal Title: PloS one 2014, Vol.9(10), p.e108660
Main Author: Armitage, Sophie A O
Other Authors: Sun, Wei , You, Xintian , Kurtz, Joachim , Schmucker, Dietmar , Chen, Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0108660
Link: http://search.proquest.com/docview/1612286105/?pq-origsite=primo
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title: Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure.
format: Article
creator:
  • Armitage, Sophie A O
  • Sun, Wei
  • You, Xintian
  • Kurtz, Joachim
  • Schmucker, Dietmar
  • Chen, Wei
subjects:
  • Alternative Splicing–Genetics
  • Animals–Metabolism
  • Drosophila Proteins–Genetics
  • Drosophila Melanogaster–Genetics
  • Escherichia Coli–Metabolism
  • Neural Cell Adhesion Molecules–Genetics
  • Protein Isoforms–Metabolism
  • Sequence Analysis, RNA–Metabolism
  • Drosophila Proteins
  • Dscam1 Protein, Drosophila
  • Neural Cell Adhesion Molecules
  • Protein Isoforms
ispartof: PloS one, 2014, Vol.9(10), p.e108660
description: The hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isoforms may be involved in the recognition of, or the defence against, diverse parasite epitopes, although evidence to support this is sparse. A prediction that can be generated from this hypothesis is that the gene expression of specific exons and/or isoforms is influenced by exposure to an immune elicitor. To test this hypothesis, we for the first time, use a long read RNA sequencing method to directly investigate the Dscam1 splicing pattern after exposing adult Drosophila melanogaster and a S2 cell line to live Escherichia coli . After bacterial exposure both models showed increased expression of immune-related genes, indicating that the immune system had been activated. However there were no changes in total Dscam1 mRNA expression. RNA sequencing further showed that there were no significant changes in individual exon expression and no changes in isoform splicing patterns in response to bacterial exposure. Therefore our studies do not support a change of D. melanogaster Dscam1 isoform diversity in response to live E. coli . Nevertheless, in future this approach could be used to identify potentially immune-related Dscam1 splicing regulation in other host species or in response to other pathogens.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0108660
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleQuantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure.
creatorArmitage, Sophie A O ; Sun, Wei ; You, Xintian ; Kurtz, Joachim ; Schmucker, Dietmar ; Chen, Wei
contributorArmitage, Sophie A O (correspondence author) ; Armitage, Sophie A O (record owner)
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0108660
subjectAlternative Splicing–Genetics ; Animals–Metabolism ; Drosophila Proteins–Genetics ; Drosophila Melanogaster–Genetics ; Escherichia Coli–Metabolism ; Neural Cell Adhesion Molecules–Genetics ; Protein Isoforms–Metabolism ; Sequence Analysis, RNA–Metabolism ; Drosophila Proteins ; Dscam1 Protein, Drosophila ; Neural Cell Adhesion Molecules ; Protein Isoforms
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descriptionThe hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isoforms may be involved in the recognition of, or the defence against, diverse parasite epitopes, although evidence to support this is sparse. A prediction that can be generated from this hypothesis is that the gene expression of specific exons and/or isoforms is influenced by exposure to an immune elicitor. To test this hypothesis, we for the first time, use a long read RNA sequencing method to directly investigate the Dscam1 splicing pattern after exposing adult Drosophila melanogaster and a S2 cell line to live Escherichia coli . After bacterial exposure both models showed increased expression of immune-related genes, indicating that the immune system had been activated. However there were no changes in total Dscam1 mRNA expression. RNA sequencing further showed that there were no significant changes in individual exon expression and no changes in isoform splicing patterns in response to bacterial exposure. Therefore our studies do not support a change of D. melanogaster Dscam1 isoform diversity in response to live E. coli . Nevertheless, in future this approach could be used to identify potentially immune-related Dscam1 splicing regulation in other host species or in response to other pathogens.
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