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Impedimetric toxicity assay in microfluidics using free and liposome-encapsulated anticancer drugs.

In this work, we have developed a microfluidic cytotoxicity assay for a cell culture and detection platform, which enables both fluid handling and electrochemical/optical detection. The cytotoxic effect of anticancer drugs doxorubicin (DOX), oxaliplatin (OX) as well as OX-loaded liposomes, developed... Full description

Journal Title: Analytical chemistry February 17, 2015, Vol.87(4), pp.2204-2212
Main Author: Caviglia, Claudia
Other Authors: Zór, Kinga , Montini, Lucia , Tilli, Valeria , Canepa, Silvia , Melander, Fredrik , Muhammad, Haseena B , Carminati, Marco , Ferrari, Giorgio , Raiteri, Roberto , Heiskanen, Arto , Andresen, Thomas L , Emnéus, Jenny
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/ac503621d
Link: http://search.proquest.com/docview/1656043834/?pq-origsite=primo
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title: Impedimetric toxicity assay in microfluidics using free and liposome-encapsulated anticancer drugs.
format: Article
creator:
  • Caviglia, Claudia
  • Zór, Kinga
  • Montini, Lucia
  • Tilli, Valeria
  • Canepa, Silvia
  • Melander, Fredrik
  • Muhammad, Haseena B
  • Carminati, Marco
  • Ferrari, Giorgio
  • Raiteri, Roberto
  • Heiskanen, Arto
  • Andresen, Thomas L
  • Emnéus, Jenny
subjects:
  • Antineoplastic Agents–Chemistry
  • Cell Death–Pharmacology
  • Dose-Response Relationship, Drug–Drug Effects
  • Doxorubicin–Chemistry
  • Drug Screening Assays, Antitumor–Pharmacology
  • Hela Cells–Chemistry
  • Humans–Instrumentation
  • Liposomes–Chemistry
  • Microfluidic Analytical Techniques–Pharmacology
  • Organoplatinum Compounds–Pharmacology
  • Structure-Activity Relationship–Pharmacology
  • Tumor Cells, Cultured–Pharmacology
  • Antineoplastic Agents
  • Liposomes
  • Organoplatinum Compounds
  • Oxaliplatin
  • Doxorubicin
ispartof: Analytical chemistry, February 17, 2015, Vol.87(4), pp.2204-2212
description: In this work, we have developed a microfluidic cytotoxicity assay for a cell culture and detection platform, which enables both fluid handling and electrochemical/optical detection. The cytotoxic effect of anticancer drugs doxorubicin (DOX), oxaliplatin (OX) as well as OX-loaded liposomes, developed for targeted drug delivery, was evaluated using real-time impedance monitoring. The time-dependent effect of DOX on HeLa cells was monitored and found to have a delayed onset of cytotoxicity in microfluidics compared with static culture conditions based on data obtained in our previous study. The result of a fluorescent microscopic annexin V/propidium iodide assay, performed in microfluidics, confirmed the outcome of the real-time impedance assay. In addition, the response of HeLa cells to OX-induced cytotoxicity proved to be slower than toxicity induced by DOX. A difference in the time-dependent cytotoxic response of fibrosarcoma cells (HT1080) to free OX and OX-loaded liposomes was observed and attributed to incomplete degradation of the liposomes, which results in lower drug availability. The matrix metalloproteinase (MMP)-dependent release of OX from OX-loaded liposomes was also confirmed using laryngopharynx carcinoma cells (FaDu). The comparison and the observed differences between the cytotoxic effects under microfluidic and static conditions highlight the importance of comparative studies as basis for implementation of microfluidic cytotoxic assays.
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/ac503621d
fulltext: no_fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleImpedimetric toxicity assay in microfluidics using free and liposome-encapsulated anticancer drugs.
creatorCaviglia, Claudia ; Zór, Kinga ; Montini, Lucia ; Tilli, Valeria ; Canepa, Silvia ; Melander, Fredrik ; Muhammad, Haseena B ; Carminati, Marco ; Ferrari, Giorgio ; Raiteri, Roberto ; Heiskanen, Arto ; Andresen, Thomas L ; Emnéus, Jenny
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subjectAntineoplastic Agents–Chemistry ; Cell Death–Pharmacology ; Dose-Response Relationship, Drug–Drug Effects ; Doxorubicin–Chemistry ; Drug Screening Assays, Antitumor–Pharmacology ; Hela Cells–Chemistry ; Humans–Instrumentation ; Liposomes–Chemistry ; Microfluidic Analytical Techniques–Pharmacology ; Organoplatinum Compounds–Pharmacology ; Structure-Activity Relationship–Pharmacology ; Tumor Cells, Cultured–Pharmacology ; Antineoplastic Agents ; Liposomes ; Organoplatinum Compounds ; Oxaliplatin ; Doxorubicin
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descriptionIn this work, we have developed a microfluidic cytotoxicity assay for a cell culture and detection platform, which enables both fluid handling and electrochemical/optical detection. The cytotoxic effect of anticancer drugs doxorubicin (DOX), oxaliplatin (OX) as well as OX-loaded liposomes, developed for targeted drug delivery, was evaluated using real-time impedance monitoring. The time-dependent effect of DOX on HeLa cells was monitored and found to have a delayed onset of cytotoxicity in microfluidics compared with static culture conditions based on data obtained in our previous study. The result of a fluorescent microscopic annexin V/propidium iodide assay, performed in microfluidics, confirmed the outcome of the real-time impedance assay. In addition, the response of HeLa cells to OX-induced cytotoxicity proved to be slower than toxicity induced by DOX. A difference in the time-dependent cytotoxic response of fibrosarcoma cells (HT1080) to free OX and OX-loaded liposomes was observed and attributed to incomplete degradation of the liposomes, which results in lower drug availability. The matrix metalloproteinase (MMP)-dependent release of OX from OX-loaded liposomes was also confirmed using laryngopharynx carcinoma cells (FaDu). The comparison and the observed differences between the cytotoxic effects under microfluidic and static conditions highlight the importance of comparative studies as basis for implementation of microfluidic cytotoxic assays.
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titleImpedimetric toxicity assay in microfluidics using free and liposome-encapsulated anticancer drugs.
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1Cell Death–Pharmacology
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10Structure-Activity Relationship–Pharmacology
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titleImpedimetric toxicity assay in microfluidics using free and liposome-encapsulated anticancer drugs.
authorCaviglia, Claudia ; Zór, Kinga ; Montini, Lucia ; Tilli, Valeria ; Canepa, Silvia ; Melander, Fredrik ; Muhammad, Haseena B ; Carminati, Marco ; Ferrari, Giorgio ; Raiteri, Roberto ; Heiskanen, Arto ; Andresen, Thomas L ; Emnéus, Jenny
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5Hela Cells–Chemistry
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9Organoplatinum Compounds–Pharmacology
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