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The frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin.

The circadian clock is controlled by a network of interconnected feedback loops that require histone modifications and chromatin remodeling. Long noncoding natural antisense transcripts (NATs) originate from Period in mammals and frequency (frq) in Neurospora. To understand the role of NATs in the c... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America April 7, 2015, Vol.112(14), pp.4357-4362
Main Author: Li, Na
Other Authors: Joska, Tammy M , Ruesch, Catherine E , Coster, Samuel J , Belden, William J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1406130112
Link: http://search.proquest.com/docview/1672090990/?pq-origsite=primo
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title: The frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin.
format: Article
creator:
  • Li, Na
  • Joska, Tammy M
  • Ruesch, Catherine E
  • Coster, Samuel J
  • Belden, William J
subjects:
  • Biological Clocks–Genetics
  • Clock Proteins–Genetics
  • Circadian Rhythm–Metabolism
  • DNA Methylation–Metabolism
  • Gene Expression Regulation–Genetics
  • Gene Expression Regulation, Fungal–Metabolism
  • Heterochromatin–Genetics
  • Histones–Genetics
  • Neurospora Crassa–Metabolism
  • Oligonucleotides, Antisense–Metabolism
  • Oscillometry–Metabolism
  • Promoter Regions, Genetic–Metabolism
  • RNA, Long Noncoding–Metabolism
  • RNA, Small Interfering–Metabolism
  • Heterochromatin
  • Histones
  • Oligonucleotides, Antisense
  • RNA, Long Noncoding
  • RNA, Small Interfering
  • Clock Proteins
  • DNA Methylation
  • Circadian Rhythm
  • Heterochromatin
  • Natural Antisense Transcripts
ispartof: Proceedings of the National Academy of Sciences of the United States of America, April 7, 2015, Vol.112(14), pp.4357-4362
description: The circadian clock is controlled by a network of interconnected feedback loops that require histone modifications and chromatin remodeling. Long noncoding natural antisense transcripts (NATs) originate from Period in mammals and frequency (frq) in Neurospora. To understand the role of NATs in the clock, we put the frq antisense transcript qrf (frq spelled backwards) under the control of an inducible promoter. Replacing the endogenous qrf promoter altered heterochromatin formation and DNA methylation at frq. In addition, constitutive, low-level induction of qrf caused a dramatic effect on the endogenous rhythm and elevated circadian output. Surprisingly, even though qrf is needed for heterochromatic silencing, induction of qrf initially promoted frq gene expression by creating a more permissible local chromatin environment. The observation that antisense expression can initially promote sense gene expression before silencing via heterochromatin formation at convergent loci is also found when a NAT to hygromycin resistance gene is driven off the endogenous vivid (vvd) promoter in the [DELTA]vvd strain. Facultative heterochromatin silencing at frq functions in a parallel pathway to previously characterized VVD-dependent silencing and is needed to establish the appropriate circadian phase. Thus, repression via dicer-independent siRNA-mediated facultative heterochromatin is largely independent of, and occurs alongside, other feedback processes. circadian rhythm | natural antisense transcripts | heterochromatin | DNA methylation
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1406130112
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titleThe frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin.
creatorLi, Na ; Joska, Tammy M ; Ruesch, Catherine E ; Coster, Samuel J ; Belden, William J
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ispartofProceedings of the National Academy of Sciences of the United States of America, April 7, 2015, Vol.112(14), pp.4357-4362
identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1406130112
subjectBiological Clocks–Genetics ; Clock Proteins–Genetics ; Circadian Rhythm–Metabolism ; DNA Methylation–Metabolism ; Gene Expression Regulation–Genetics ; Gene Expression Regulation, Fungal–Metabolism ; Heterochromatin–Genetics ; Histones–Genetics ; Neurospora Crassa–Metabolism ; Oligonucleotides, Antisense–Metabolism ; Oscillometry–Metabolism ; Promoter Regions, Genetic–Metabolism ; RNA, Long Noncoding–Metabolism ; RNA, Small Interfering–Metabolism ; Heterochromatin ; Histones ; Oligonucleotides, Antisense ; RNA, Long Noncoding ; RNA, Small Interfering ; Clock Proteins ; DNA Methylation ; Circadian Rhythm ; Heterochromatin ; Natural Antisense Transcripts
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descriptionThe circadian clock is controlled by a network of interconnected feedback loops that require histone modifications and chromatin remodeling. Long noncoding natural antisense transcripts (NATs) originate from Period in mammals and frequency (frq) in Neurospora. To understand the role of NATs in the clock, we put the frq antisense transcript qrf (frq spelled backwards) under the control of an inducible promoter. Replacing the endogenous qrf promoter altered heterochromatin formation and DNA methylation at frq. In addition, constitutive, low-level induction of qrf caused a dramatic effect on the endogenous rhythm and elevated circadian output. Surprisingly, even though qrf is needed for heterochromatic silencing, induction of qrf initially promoted frq gene expression by creating a more permissible local chromatin environment. The observation that antisense expression can initially promote sense gene expression before silencing via heterochromatin formation at convergent loci is also found when a NAT to hygromycin resistance gene is driven off the endogenous vivid (vvd) promoter in the [DELTA]vvd strain. Facultative heterochromatin silencing at frq functions in a parallel pathway to previously characterized VVD-dependent silencing and is needed to establish the appropriate circadian phase. Thus, repression via dicer-independent siRNA-mediated facultative heterochromatin is largely independent of, and occurs alongside, other feedback processes. circadian rhythm | natural antisense transcripts | heterochromatin | DNA methylation
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