schliessen

Filtern

 

Bibliotheken

Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.

Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effect... Full description

Journal Title: The Journal of reproduction and development 2015, Vol.61(2), pp.81-89
Main Author: Yeon, Ji-Yeong
Other Authors: Min, Sung-Hun , Park, Hyo-Jin , Kim, Jin-Woo , Lee, Yong-Hee , Park, Soo-Yong , Jeong, Pil-Soo , Park, Humdai , Lee, Dong-Seok , Kim, Sun-Uk , Chang, Kyu-Tae , Koo, Deog-Bon
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1348-4400 ; DOI: 10.1262/jrd.2014-070
Link: http://search.proquest.com/docview/1674688771/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1674688771
title: Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.
format: Article
creator:
  • Yeon, Ji-Yeong
  • Min, Sung-Hun
  • Park, Hyo-Jin
  • Kim, Jin-Woo
  • Lee, Yong-Hee
  • Park, Soo-Yong
  • Jeong, Pil-Soo
  • Park, Humdai
  • Lee, Dong-Seok
  • Kim, Sun-Uk
  • Chang, Kyu-Tae
  • Koo, Deog-Bon
subjects:
  • Animals–Drug Effects
  • Apoptosis–Physiology
  • Death-Associated Protein Kinases–Metabolism
  • Embryo Culture Techniques–Drug Effects
  • Embryonic Development–Physiology
  • Membrane Potential, Mitochondrial–Drug Effects
  • Mitochondria–Physiology
  • Mitochondrial Dynamics–Drug Effects
  • Quinazolinones–Metabolism
  • Reactive Oxygen Species–Drug Effects
  • Swine–Physiology
  • Swine–Pharmacology
  • Swine–Metabolism
  • 3-(2,4-Dichloro-5-Methoxyphenyl)-2-Sulfanyl-4(3h)-Quinazolinone
  • Quinazolinones
  • Reactive Oxygen Species
  • Death-Associated Protein Kinases
ispartof: The Journal of reproduction and development, 2015, Vol.61(2), pp.81-89
description: Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.
language: eng
source:
identifier: E-ISSN: 1348-4400 ; DOI: 10.1262/jrd.2014-070
fulltext: fulltext
issn:
  • 13484400
  • 1348-4400
url: Link


@attributes
ID13850002
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1674688771
sourceidproquest
recordidTN_proquest1674688771
sourcesystemPC
pqid1674688771
display
typearticle
titleMdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.
creatorYeon, Ji-Yeong ; Min, Sung-Hun ; Park, Hyo-Jin ; Kim, Jin-Woo ; Lee, Yong-Hee ; Park, Soo-Yong ; Jeong, Pil-Soo ; Park, Humdai ; Lee, Dong-Seok ; Kim, Sun-Uk ; Chang, Kyu-Tae ; Koo, Deog-Bon
contributorYeon, Ji-Yeong (correspondence author) ; Yeon, Ji-Yeong (record owner)
ispartofThe Journal of reproduction and development, 2015, Vol.61(2), pp.81-89
identifierE-ISSN: 1348-4400 ; DOI: 10.1262/jrd.2014-070
subjectAnimals–Drug Effects ; Apoptosis–Physiology ; Death-Associated Protein Kinases–Metabolism ; Embryo Culture Techniques–Drug Effects ; Embryonic Development–Physiology ; Membrane Potential, Mitochondrial–Drug Effects ; Mitochondria–Physiology ; Mitochondrial Dynamics–Drug Effects ; Quinazolinones–Metabolism ; Reactive Oxygen Species–Drug Effects ; Swine–Physiology ; Swine–Pharmacology ; Swine–Metabolism ; 3-(2,4-Dichloro-5-Methoxyphenyl)-2-Sulfanyl-4(3h)-Quinazolinone ; Quinazolinones ; Reactive Oxygen Species ; Death-Associated Protein Kinases
languageeng
source
descriptionMitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. These dynamic mitochondrial processes influence mitochondrial morphology, size and function. Therefore, this study was conducted to evaluate the effects of mitochondrial fission inhibitor, mdivi-1, on developmental competence and mitochondrial function of porcine embryos and primary cells. Presumptive porcine embryos were cultured in PZM-3 medium supplemented with mdivi-1 (0, 10 and 50 μM) for 6 days. Porcine fibroblast cells were cultured in growth medium with mdivi-1 (0 and 50 μM) for 2 days. Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < 0.05). Moreover, loss of mitochondrial membrane potential in the mdivi-1 (50 μM) treated group was increased relative to the control group (P < 0.05). Subsequent evaluation revealed that the intracellular levels of reactive oxygen species (ROS) and the apoptotic index were increased by mdivi-1 (50 μM) treatment (P < 0.05). Finally, the expression of mitochondrial fission-related protein (Drp 1) was lower in the embryos and cells in the mdivi-1-treated group than the control group. Taken together, these results indicate that mdivi-1 treatment may inhibit developmental competence and mitochondrial function in porcine embryos and primary cells.
version5
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1674688771/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Yeon, Ji-Yeong
1Min, Sung-Hun
2Park, Hyo-Jin
3Kim, Jin-Woo
4Lee, Yong-Hee
5Park, Soo-Yong
6Jeong, Pil-Soo
7Park, Humdai
8Lee, Dong-Seok
9Kim, Sun-Uk
10Chang, Kyu-Tae
11Koo, Deog-Bon
titleMdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.
subject
0Animals–Drug Effects
1Apoptosis–Physiology
2Death-Associated Protein Kinases–Metabolism
3Embryo Culture Techniques–Drug Effects
4Embryonic Development–Physiology
5Membrane Potential, Mitochondrial–Drug Effects
6Mitochondria–Physiology
7Mitochondrial Dynamics–Drug Effects
8Quinazolinones–Metabolism...
general
0English
110.1262/jrd.2014-070
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest1674688771
issn
013484400
11348-4400
rsrctypearticle
creationdate2015
addtitleThe Journal of reproduction and development
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorYeon, Ji-Yeong
startdate20150101
enddate20150101
citationpf 81 pt 89 vol 61 issue 2
lsr30VSR-Enriched:[description, pqid, issn, date]
sort
titleMdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.
authorYeon, Ji-Yeong ; Min, Sung-Hun ; Park, Hyo-Jin ; Kim, Jin-Woo ; Lee, Yong-Hee ; Park, Soo-Yong ; Jeong, Pil-Soo ; Park, Humdai ; Lee, Dong-Seok ; Kim, Sun-Uk ; Chang, Kyu-Tae ; Koo, Deog-Bon
creationdate20150101
lso0120150101
facets
frbrgroupid355708592468232512
frbrtype5
newrecords20181218
languageeng
creationdate2015
topic
0Animals–Drug Effects
1Apoptosis–Physiology
2Death-Associated Protein Kinases–Metabolism
3Embryo Culture Techniques–Drug Effects
4Embryonic Development–Physiology
5Membrane Potential, Mitochondrial–Drug Effects
6Mitochondria–Physiology
7Mitochondrial Dynamics–Drug Effects
8Quinazolinones–Metabolism...
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Yeon, Ji-Yeong
1Min, Sung-Hun
2Park, Hyo-Jin
3Kim, Jin-Woo
4Lee, Yong-Hee
5Park, Soo-Yong
6Jeong, Pil-Soo
7Park, Humdai
8Lee, Dong-Seok
9Kim, Sun-Uk
10Chang, Kyu-Tae
11Koo, Deog-Bon
jtitleJournal of reproduction and development
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Yeon
1Min
2Park
3Kim
4Lee
5Jeong
6Chang
7Koo
aufirst
0Ji-Yeong
1Sung-Hun
2Hyo-Jin
3Jin-Woo
4Yong-Hee
5Soo-Yong
6Pil-Soo
7Humdai
8Dong-Seok
9Sun-Uk
10Kyu-Tae
11Deog-Bon
au
0Yeon, Ji-Yeong
1Min, Sung-Hun
2Park, Hyo-Jin
3Kim, Jin-Woo
4Lee, Yong-Hee
5Park, Soo-Yong
6Jeong, Pil-Soo
7Park, Humdai
8Lee, Dong-Seok
9Kim, Sun-Uk
10Chang, Kyu-Tae
11Koo, Deog-Bon
addauYeon, Ji-Yeong
atitleMdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs.
jtitleThe Journal of reproduction and development
date2015
risdate20150101
volume61
issue2
spage81
epage89
pages81-89
eissn1348-4400
formatjournal
genrearticle
ristypeJOUR
doi10.1262/jrd.2014-070
urlhttp://search.proquest.com/docview/1674688771/
issn09168818