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Plasmid-Encoded Pgp5 Is a Significant Contributor to Chlamydia muridarum Induction of Hydrosalpinx.

We have previously shown that the plasmid-encoded Pgp3 is a major virulence factor for C . muridarum induction of hydrosalpinx. We now report that Pgp5 also plays a significant role in the development of hydrosalpinx following C . muridarum induction. Pgp5 deficiency was introduced via either in-fra... Full description

Journal Title: PloS one 2015, Vol.10(4), p.e0124840
Main Author: Huang, Yumeng
Other Authors: Zhang, Qi , Yang, Zhangsheng , Conrad, Turner , Liu, Yuanjun , Zhong, Guangming
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0124840
Link: http://search.proquest.com/docview/1677886822/?pq-origsite=primo
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title: Plasmid-Encoded Pgp5 Is a Significant Contributor to Chlamydia muridarum Induction of Hydrosalpinx.
format: Article
creator:
  • Huang, Yumeng
  • Zhang, Qi
  • Yang, Zhangsheng
  • Conrad, Turner
  • Liu, Yuanjun
  • Zhong, Guangming
subjects:
  • Animals–Microbiology
  • Chlamydia Infections–Genetics
  • Chlamydia Muridarum–Pathogenicity
  • Fallopian Tubes–Microbiology
  • Female–Genetics
  • Glycoproteins–Metabolism
  • Mice–Genetics
  • Plasmids–Genetics
  • Virulence Factors–Metabolism
  • Glycoproteins
  • Virulence Factors
ispartof: PloS one, 2015, Vol.10(4), p.e0124840
description: We have previously shown that the plasmid-encoded Pgp3 is a major virulence factor for C . muridarum induction of hydrosalpinx. We now report that Pgp5 also plays a significant role in the development of hydrosalpinx following C . muridarum induction. Pgp5 deficiency was introduced via either in-frame deletion (CM-Δpgp5) or premature stop codon installation (CM-pgp5S). Mice infected with either CM-Δpgp5 or CM-pgp5S developed hydrosalpinges at significantly reduced levels with an incidence rate of 3 when mice were infected with Pgp5-sufficient C . muridarum (plasmid-competent wild type or plasmid-free C . muridarum transformed with a full plasmid or depleted of pgp7 gene). The attenuated pathogenicity of the Pgp5-deficient C . muridarum correlated with a significantly reduced level of ascending infection in the oviduct tissue despite the similar overall shedding courses between mice infected with Pgp5-deficeint versus sufficient C . muridarum . Furthermore, in the oviducts of mice infected with Pgp5-deficient C . muridarum , significantly lower levels of inflammatory cell infiltration and cytokine production were detected. Thus, Pgp5 is a significant plasmid-encoded virulence factor for C . muridarum pathogenicity in the upper genital tract.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0124840
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titlePlasmid-Encoded Pgp5 Is a Significant Contributor to Chlamydia muridarum Induction of Hydrosalpinx.
creatorHuang, Yumeng ; Zhang, Qi ; Yang, Zhangsheng ; Conrad, Turner ; Liu, Yuanjun ; Zhong, Guangming
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0124840
subjectAnimals–Microbiology ; Chlamydia Infections–Genetics ; Chlamydia Muridarum–Pathogenicity ; Fallopian Tubes–Microbiology ; Female–Genetics ; Glycoproteins–Metabolism ; Mice–Genetics ; Plasmids–Genetics ; Virulence Factors–Metabolism ; Glycoproteins ; Virulence Factors
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descriptionWe have previously shown that the plasmid-encoded Pgp3 is a major virulence factor for C . muridarum induction of hydrosalpinx. We now report that Pgp5 also plays a significant role in the development of hydrosalpinx following C . muridarum induction. Pgp5 deficiency was introduced via either in-frame deletion (CM-Δpgp5) or premature stop codon installation (CM-pgp5S). Mice infected with either CM-Δpgp5 or CM-pgp5S developed hydrosalpinges at significantly reduced levels with an incidence rate of <40% and a mean severity score of 2 or less. In contrast, 80% or more mice developed hydrosalpinx with a severity score of >3 when mice were infected with Pgp5-sufficient C . muridarum (plasmid-competent wild type or plasmid-free C . muridarum transformed with a full plasmid or depleted of pgp7 gene). The attenuated pathogenicity of the Pgp5-deficient C . muridarum correlated with a significantly reduced level of ascending infection in the oviduct tissue despite the similar overall shedding courses between mice infected with Pgp5-deficeint versus sufficient C . muridarum . Furthermore, in the oviducts of mice infected with Pgp5-deficient C . muridarum , significantly lower levels of inflammatory cell infiltration and cytokine production were detected. Thus, Pgp5 is a significant plasmid-encoded virulence factor for C . muridarum pathogenicity in the upper genital tract.
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