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Up-Regulation of the Biosynthesis and Release of Substance P through Wnt/β-Catenin Signaling Pathway in Rat Dorsal Root Ganglion Cells.

To examine regulatory effects of beta -catenin on the biosynthesis and release of substance P, a rat chronic constriction injury (CCI) model and a rat dorsal root ganglion (DRG) cell culture model were used in the present study. The CCI treatment significantly induced the overall expression of beta... Full description

Journal Title: PloS one 2015, Vol.10(6), p.e0129701
Main Author: Li, Yu-Sang
Other Authors: Xi, Yang , Li, Xiao-Jun , Leng, Chang-Long , Jia, Mei-Mei , Zhang, Wei Kevin , Tang, He-Bin
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0129701
Link: http://search.proquest.com/docview/1687361572/?pq-origsite=primo
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title: Up-Regulation of the Biosynthesis and Release of Substance P through Wnt/β-Catenin Signaling Pathway in Rat Dorsal Root Ganglion Cells.
format: Article
creator:
  • Li, Yu-Sang
  • Xi, Yang
  • Li, Xiao-Jun
  • Leng, Chang-Long
  • Jia, Mei-Mei
  • Zhang, Wei Kevin
  • Tang, He-Bin
subjects:
  • Animals–Metabolism
  • Cell Nucleus–Genetics
  • Cells, Cultured–Metabolism
  • Cyclooxygenase 2–Cytology
  • Disease Models, Animal–Metabolism
  • Ganglia, Spinal–Metabolism
  • Intracellular Space–Genetics
  • Male–Metabolism
  • Peripheral Nerve Injuries–Metabolism
  • Protein Transport–Genetics
  • Rats–Metabolism
  • Substance P–Metabolism
  • Transcription, Genetic–Metabolism
  • Wnt Signaling Pathway–Metabolism
  • Beta Catenin–Metabolism
  • Beta Catenin
  • Substance P
  • Cyclooxygenase 2
ispartof: PloS one, 2015, Vol.10(6), p.e0129701
description: To examine regulatory effects of beta -catenin on the biosynthesis and release of substance P, a rat chronic constriction injury (CCI) model and a rat dorsal root ganglion (DRG) cell culture model were used in the present study. The CCI treatment significantly induced the overall expression of beta -catenin (158 plus or minus 6% of sham) in the ipsilateral L5 DRGs in comparison with the sham group (109 plus or minus 4% of sham). The CCI-induced aberrant expression of beta -catenin was significantly attenuated by oral administration of diclofenac (119 plus or minus 6% of the sham value; 10 mg/kg). Importantly, aberrant nuclear accumulation of beta -catenin in cultured DRG cells resulted in up-regulation of the PPT-A mRNA expression and the substance P release. The up-regulation of both the PPT-A mRNA expression and the substance P release by either a GSK-3 beta inhibitor TWS119 (10 mu M) or a Wnt signaling agonist Wnt-3a (100 ng/ml) were significantly abolished by an inhibitor of cyclooxygenase-2 (COX-2; NS-398, 1 mu M). Collectively, these data suggest that nociceptive input-activated beta -catenin signaling plays an important role in regulating the biosynthesis and release of substance P, which may contribute to the inflammation responses related to chronic pain.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0129701
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleUp-Regulation of the Biosynthesis and Release of Substance P through Wnt/β-Catenin Signaling Pathway in Rat Dorsal Root Ganglion Cells.
creatorLi, Yu-Sang ; Xi, Yang ; Li, Xiao-Jun ; Leng, Chang-Long ; Jia, Mei-Mei ; Zhang, Wei Kevin ; Tang, He-Bin
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0129701
subjectAnimals–Metabolism ; Cell Nucleus–Genetics ; Cells, Cultured–Metabolism ; Cyclooxygenase 2–Cytology ; Disease Models, Animal–Metabolism ; Ganglia, Spinal–Metabolism ; Intracellular Space–Genetics ; Male–Metabolism ; Peripheral Nerve Injuries–Metabolism ; Protein Transport–Genetics ; Rats–Metabolism ; Substance P–Metabolism ; Transcription, Genetic–Metabolism ; Wnt Signaling Pathway–Metabolism ; Beta Catenin–Metabolism ; Beta Catenin ; Substance P ; Cyclooxygenase 2
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descriptionTo examine regulatory effects of beta -catenin on the biosynthesis and release of substance P, a rat chronic constriction injury (CCI) model and a rat dorsal root ganglion (DRG) cell culture model were used in the present study. The CCI treatment significantly induced the overall expression of beta -catenin (158 plus or minus 6% of sham) in the ipsilateral L5 DRGs in comparison with the sham group (109 plus or minus 4% of sham). The CCI-induced aberrant expression of beta -catenin was significantly attenuated by oral administration of diclofenac (119 plus or minus 6% of the sham value; 10 mg/kg). Importantly, aberrant nuclear accumulation of beta -catenin in cultured DRG cells resulted in up-regulation of the PPT-A mRNA expression and the substance P release. The up-regulation of both the PPT-A mRNA expression and the substance P release by either a GSK-3 beta inhibitor TWS119 (10 mu M) or a Wnt signaling agonist Wnt-3a (100 ng/ml) were significantly abolished by an inhibitor of cyclooxygenase-2 (COX-2; NS-398, 1 mu M). Collectively, these data suggest that nociceptive input-activated beta -catenin signaling plays an important role in regulating the biosynthesis and release of substance P, which may contribute to the inflammation responses related to chronic pain.
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