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RNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel).

The transformer ( tra ) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 ( tra-2 ) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster . In this study, we describe the isolation, expression and fun... Full description

Journal Title: PloS one 2015, Vol.10(6), p.e0128892
Main Author: Liu, Guiqing
Other Authors: Wu, Qiang , Li, Jianwei , Zhang, Guifen , Wan, Fanghao
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0128892
Link: http://search.proquest.com/docview/1687649120/?pq-origsite=primo
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title: RNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel).
format: Article
creator:
  • Liu, Guiqing
  • Wu, Qiang
  • Li, Jianwei
  • Zhang, Guifen
  • Wan, Fanghao
subjects:
  • Animals–Genetics
  • Diptera–Physiology
  • Female–Genetics
  • Gene Silencing–Metabolism
  • Insect Proteins–Genetics
  • Male–Metabolism
  • RNA-Binding Proteins–Genetics
  • Sex Determination Processes–Genetics
  • Sex Differentiation–Genetics
  • Insect Proteins
  • RNA-Binding Proteins
ispartof: PloS one, 2015, Vol.10(6), p.e0128892
description: The transformer ( tra ) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 ( tra-2 ) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster . In this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, Bactrocera dorsalis (Hendel). Bdtra and Bdtra-2 are similar to their homologs from other tephritid species. Bdtra demonstrated sex-specific transcripts: one transcript in females and two transcripts in males. In contrast, Bdtra-2 only had one transcript that was common to males and females, which was transcribed continuously in different adult tissues and developmental stages. Bdtra-2 and the female form of Bdtra were maternally inherited in eggs, whereas the male form of Bdtra was not detectable until embryos of 1 and 2 h after egg laying. Function analyses of Bdtra and Bdtra-2 indicated that both were indispensable for female development, as nearly 100% males were obtained with embryonic RNAi against either Bdtra or Bdtra-2 . The fertility of these RNAi-generated males was subsequently tested. More than 80% of RNAi-generated males could mate and the mated females could lay eggs, but only 40-48.6% males gave rise to progeny. In XX-reversed males and intersex individuals, no clear female gonadal morphology was observed after dissection. These results shed light on the development of a genetic sexing system with male-only release for this agricultural pest.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0128892
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleRNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel).
creatorLiu, Guiqing ; Wu, Qiang ; Li, Jianwei ; Zhang, Guifen ; Wan, Fanghao
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0128892
subjectAnimals–Genetics ; Diptera–Physiology ; Female–Genetics ; Gene Silencing–Metabolism ; Insect Proteins–Genetics ; Male–Metabolism ; RNA-Binding Proteins–Genetics ; Sex Determination Processes–Genetics ; Sex Differentiation–Genetics ; Insect Proteins ; RNA-Binding Proteins
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descriptionThe transformer ( tra ) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 ( tra-2 ) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster . In this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, Bactrocera dorsalis (Hendel). Bdtra and Bdtra-2 are similar to their homologs from other tephritid species. Bdtra demonstrated sex-specific transcripts: one transcript in females and two transcripts in males. In contrast, Bdtra-2 only had one transcript that was common to males and females, which was transcribed continuously in different adult tissues and developmental stages. Bdtra-2 and the female form of Bdtra were maternally inherited in eggs, whereas the male form of Bdtra was not detectable until embryos of 1 and 2 h after egg laying. Function analyses of Bdtra and Bdtra-2 indicated that both were indispensable for female development, as nearly 100% males were obtained with embryonic RNAi against either Bdtra or Bdtra-2 . The fertility of these RNAi-generated males was subsequently tested. More than 80% of RNAi-generated males could mate and the mated females could lay eggs, but only 40-48.6% males gave rise to progeny. In XX-reversed males and intersex individuals, no clear female gonadal morphology was observed after dissection. These results shed light on the development of a genetic sexing system with male-only release for this agricultural pest.
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