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Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp.

Bacteriophages UAB_Phi20, UAB_Phi78, and UAB_Phi87 were encapsulated in liposomes, and their efficacy in reducing Salmonella in poultry was then studied. The encapsulated phages had a mean diameter of 309 to 326 nm and a positive charge between +31.6 and +35.1 mV (pH 6.1). In simulated gastric fluid... Full description

Journal Title: Applied and environmental microbiology July 2015, Vol.81(14), pp.4841-4849
Main Author: Colom, Joan
Other Authors: Cano-Sarabia, Mary , Otero, Jennifer , Cortés, Pilar , Maspoch, Daniel , Llagostera, Montserrat
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1098-5336 ; DOI: 1098-5336 ; DOI: 10.1128/AEM.00812-15
Link: http://search.proquest.com/docview/1690210274/?pq-origsite=primo
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recordid: proquest1690210274
title: Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp.
format: Article
creator:
  • Colom, Joan
  • Cano-Sarabia, Mary
  • Otero, Jennifer
  • Cortés, Pilar
  • Maspoch, Daniel
  • Llagostera, Montserrat
subjects:
  • Animals–Instrumentation
  • Biological Therapy–Methods
  • Chickens–Chemistry
  • Liposomes–Microbiology
  • Poultry Diseases–Therapy
  • Salmonella–Physiology
  • Salmonella Infections, Animal–Virology
  • Salmonella Phages–Microbiology
  • Salmonella Phages–Therapy
  • Salmonella Phages–Chemistry
  • Salmonella Phages–Physiology
  • Liposomes
ispartof: Applied and environmental microbiology, July 2015, Vol.81(14), pp.4841-4849
description: Bacteriophages UAB_Phi20, UAB_Phi78, and UAB_Phi87 were encapsulated in liposomes, and their efficacy in reducing Salmonella in poultry was then studied. The encapsulated phages had a mean diameter of 309 to 326 nm and a positive charge between +31.6 and +35.1 mV (pH 6.1). In simulated gastric fluid (pH 2.8), the titer of nonencapsulated phages decreased by 5.7 to 7.8 log units, whereas encapsulated phages were significantly more stable, with losses of 3.7 to 5.4 log units. The liposome coating also improved the retention of bacteriophages in the chicken intestinal tract. When cocktails of the encapsulated and nonencapsulated phages were administered to broilers, after 72 h the encapsulated phages were detected in 38.1% of the animals, whereas the nonencapsulated phages were present in only 9.5%. The difference was significant. In addition, in an in vitro experiment, the cecal contents of broilers promoted the release of the phages from the liposomes. In broilers experimentally infected with Salmonella, the daily administration of the two cocktails for 6 days postinfection conferred similar levels of protection against Salmonella colonization. However, once treatment was stopped, protection by the nonencapsulated phages disappeared, whereas that provided by the encapsulated phages persisted for at least 1 week, showing the enhanced efficacy of the encapsulated phages in protecting poultry against Salmonella over time. The methodology described here allows the liposome encapsulation of phages of different morphologies. The preparations can be stored for at least 3 months at 4...C and could be added to the drinking water and feed of animals. (ProQuest: ... denotes formulae/symbols omitted.)
language: eng
source:
identifier: E-ISSN: 1098-5336 ; DOI: 1098-5336 ; DOI: 10.1128/AEM.00812-15
fulltext: fulltext
issn:
  • 10985336
  • 1098-5336
url: Link


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creatorColom, Joan ; Cano-Sarabia, Mary ; Otero, Jennifer ; Cortés, Pilar ; Maspoch, Daniel ; Llagostera, Montserrat
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subjectAnimals–Instrumentation ; Biological Therapy–Methods ; Chickens–Chemistry ; Liposomes–Microbiology ; Poultry Diseases–Therapy ; Salmonella–Physiology ; Salmonella Infections, Animal–Virology ; Salmonella Phages–Microbiology ; Salmonella Phages–Therapy ; Salmonella Phages–Chemistry ; Salmonella Phages–Physiology ; Liposomes
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descriptionBacteriophages UAB_Phi20, UAB_Phi78, and UAB_Phi87 were encapsulated in liposomes, and their efficacy in reducing Salmonella in poultry was then studied. The encapsulated phages had a mean diameter of 309 to 326 nm and a positive charge between +31.6 and +35.1 mV (pH 6.1). In simulated gastric fluid (pH 2.8), the titer of nonencapsulated phages decreased by 5.7 to 7.8 log units, whereas encapsulated phages were significantly more stable, with losses of 3.7 to 5.4 log units. The liposome coating also improved the retention of bacteriophages in the chicken intestinal tract. When cocktails of the encapsulated and nonencapsulated phages were administered to broilers, after 72 h the encapsulated phages were detected in 38.1% of the animals, whereas the nonencapsulated phages were present in only 9.5%. The difference was significant. In addition, in an in vitro experiment, the cecal contents of broilers promoted the release of the phages from the liposomes. In broilers experimentally infected with Salmonella, the daily administration of the two cocktails for 6 days postinfection conferred similar levels of protection against Salmonella colonization. However, once treatment was stopped, protection by the nonencapsulated phages disappeared, whereas that provided by the encapsulated phages persisted for at least 1 week, showing the enhanced efficacy of the encapsulated phages in protecting poultry against Salmonella over time. The methodology described here allows the liposome encapsulation of phages of different morphologies. The preparations can be stored for at least 3 months at 4...C and could be added to the drinking water and feed of animals. (ProQuest: ... denotes formulae/symbols omitted.)
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