schliessen

Filtern

 

Bibliotheken

CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.

Glioblastoma (GB) remains the most aggressive primary brain malignancy. Adoptive transfer of chimeric antigen receptor (CAR)-modified immune cells has emerged as a promising anti-cancer approach, yet the potential utility of CAR-engineered natural killer (NK) cells to treat GB has not been explored.... Full description

Journal Title: Scientific reports July 9, 2015, Vol.5, p.11483
Main Author: Han, Jianfeng
Other Authors: Chu, Jianhong , Keung Chan, Wing , Zhang, Jianying , Wang, Youwei , Cohen, Justus B , Victor, Aaron , Meisen, Walter H , Kim, Sung-Hak , Grandi, Paola , Wang, Qi-En , He, Xiaoming , Nakano, Ichiro , Chiocca, E Antonio , Glorioso Iii, Joseph C , Kaur, Balveen , Caligiuri, Michael A , Yu, Jianhua
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 2045-2322 ; DOI: 10.1038/srep11483
Link: http://search.proquest.com/docview/1695182613/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1695182613
title: CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.
format: Article
creator:
  • Han, Jianfeng
  • Chu, Jianhong
  • Keung Chan, Wing
  • Zhang, Jianying
  • Wang, Youwei
  • Cohen, Justus B
  • Victor, Aaron
  • Meisen, Walter H
  • Kim, Sung-Hak
  • Grandi, Paola
  • Wang, Qi-En
  • He, Xiaoming
  • Nakano, Ichiro
  • Chiocca, E Antonio
  • Glorioso Iii, Joseph C
  • Kaur, Balveen
  • Caligiuri, Michael A
  • Yu, Jianhua
subjects:
  • Animals–Genetics
  • Cell Line, Tumor–Immunology
  • Cell Movement–Genetics
  • Cytotoxicity, Immunologic–Immunology
  • Disease Models, Animal–Metabolism
  • Erbb Receptors–Genetics
  • Gene Expression–Immunology
  • Glioblastoma–Pathology
  • Humans–Biosynthesis
  • Interferon-Gamma–Immunology
  • Killer Cells, Natural–Metabolism
  • Mice–Immunology
  • Neoplastic Stem Cells–Metabolism
  • Receptors, Antigen, T-Cell–Genetics
  • Recombinant Fusion Proteins–Genetics
  • Xenograft Model Antitumor Assays–Immunology
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • Epidermal Growth Factor Receptor VIII
  • Interferon-Gamma
  • Erbb Receptors
ispartof: Scientific reports, July 9, 2015, Vol.5, p.11483
description: Glioblastoma (GB) remains the most aggressive primary brain malignancy. Adoptive transfer of chimeric antigen receptor (CAR)-modified immune cells has emerged as a promising anti-cancer approach, yet the potential utility of CAR-engineered natural killer (NK) cells to treat GB has not been explored. Tumors from approximately 50% of GB patients express wild-type EGFR (wtEGFR) and in fewer cases express both wtEGFR and the mutant form EGFRvIII; however, previously reported CAR T cell studies only focus on targeting EGFRvIII. Here we explore whether both wtEGFR and EGFRvIII can be effectively targeted by CAR-redirected NK cells to treat GB. We transduced human NK cell lines NK-92 and NKL, and primary NK cells with a lentiviral construct harboring a second generation CAR targeting both wtEGFR and EGFRvIII and evaluated the anti-GB efficacy of EGFR-CAR-modified NK cells. EGFR-CAR-engineered NK cells displayed enhanced cytolytic capability and IFN-γ production when co-cultured with GB cells or patient-derived GB stem cells in an EGFR-dependent manner. In two orthotopic GB xenograft mouse models, intracranial administration of NK-92-EGFR-CAR cells resulted in efficient suppression of tumor growth and significantly prolonged the tumor-bearing mice survival. These findings support intracranial administration of NK-92-EGFR-CAR cells represents a promising clinical strategy to treat GB.
language: eng
source:
identifier: E-ISSN: 2045-2322 ; DOI: 10.1038/srep11483
fulltext: fulltext
issn:
  • 20452322
  • 2045-2322
url: Link


@attributes
ID1350767289
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1695182613
sourceidproquest
recordidTN_proquest1695182613
sourcesystemPC
pqid1695182613
display
typearticle
titleCAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.
creatorHan, Jianfeng ; Chu, Jianhong ; Keung Chan, Wing ; Zhang, Jianying ; Wang, Youwei ; Cohen, Justus B ; Victor, Aaron ; Meisen, Walter H ; Kim, Sung-Hak ; Grandi, Paola ; Wang, Qi-En ; He, Xiaoming ; Nakano, Ichiro ; Chiocca, E Antonio ; Glorioso Iii, Joseph C ; Kaur, Balveen ; Caligiuri, Michael A ; Yu, Jianhua
contributorHan, Jianfeng (correspondence author) ; Han, Jianfeng (record owner)
ispartofScientific reports, July 9, 2015, Vol.5, p.11483
identifierE-ISSN: 2045-2322 ; DOI: 10.1038/srep11483
subjectAnimals–Genetics ; Cell Line, Tumor–Immunology ; Cell Movement–Genetics ; Cytotoxicity, Immunologic–Immunology ; Disease Models, Animal–Metabolism ; Erbb Receptors–Genetics ; Gene Expression–Immunology ; Glioblastoma–Pathology ; Humans–Biosynthesis ; Interferon-Gamma–Immunology ; Killer Cells, Natural–Metabolism ; Mice–Immunology ; Neoplastic Stem Cells–Metabolism ; Receptors, Antigen, T-Cell–Genetics ; Recombinant Fusion Proteins–Genetics ; Xenograft Model Antitumor Assays–Immunology ; Receptors, Antigen, T-Cell ; Recombinant Fusion Proteins ; Epidermal Growth Factor Receptor VIII ; Interferon-Gamma ; Erbb Receptors
languageeng
source
descriptionGlioblastoma (GB) remains the most aggressive primary brain malignancy. Adoptive transfer of chimeric antigen receptor (CAR)-modified immune cells has emerged as a promising anti-cancer approach, yet the potential utility of CAR-engineered natural killer (NK) cells to treat GB has not been explored. Tumors from approximately 50% of GB patients express wild-type EGFR (wtEGFR) and in fewer cases express both wtEGFR and the mutant form EGFRvIII; however, previously reported CAR T cell studies only focus on targeting EGFRvIII. Here we explore whether both wtEGFR and EGFRvIII can be effectively targeted by CAR-redirected NK cells to treat GB. We transduced human NK cell lines NK-92 and NKL, and primary NK cells with a lentiviral construct harboring a second generation CAR targeting both wtEGFR and EGFRvIII and evaluated the anti-GB efficacy of EGFR-CAR-modified NK cells. EGFR-CAR-engineered NK cells displayed enhanced cytolytic capability and IFN-γ production when co-cultured with GB cells or patient-derived GB stem cells in an EGFR-dependent manner. In two orthotopic GB xenograft mouse models, intracranial administration of NK-92-EGFR-CAR cells resulted in efficient suppression of tumor growth and significantly prolonged the tumor-bearing mice survival. These findings support intracranial administration of NK-92-EGFR-CAR cells represents a promising clinical strategy to treat GB.
version7
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1695182613/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Han, Jianfeng
1Chu, Jianhong
2Keung Chan, Wing
3Zhang, Jianying
4Wang, Youwei
5Cohen, Justus B
6Victor, Aaron
7Meisen, Walter H
8Kim, Sung-Hak
9Grandi, Paola
10Wang, Qi-En
11He, Xiaoming
12Nakano, Ichiro
13Chiocca, E Antonio
14Glorioso Iii, Joseph C
15Kaur, Balveen
16Caligiuri, Michael A
17Yu, Jianhua
titleCAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.
subject
0Animals–Genetics
1Cell Line, Tumor–Immunology
2Cell Movement–Genetics
3Cytotoxicity, Immunologic–Immunology
4Disease Models, Animal–Metabolism
5Erbb Receptors–Genetics
6Gene Expression–Immunology
7Glioblastoma–Pathology
8Humans–Biosynthesis
9Interferon-Gamma–Immunology
10Killer Cells, Natural–Metabolism...
general
0English
110.1038/srep11483
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest1695182613
issn
020452322
12045-2322
rsrctypearticle
creationdate2015
addtitleScientific reports
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorHan, Jianfeng
startdate20150709
enddate20150709
citationpf 11483 pt 11483 vol 5
lsr30VSR-Enriched:[description, issue, pqid]
sort
titleCAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.
authorHan, Jianfeng ; Chu, Jianhong ; Keung Chan, Wing ; Zhang, Jianying ; Wang, Youwei ; Cohen, Justus B ; Victor, Aaron ; Meisen, Walter H ; Kim, Sung-Hak ; Grandi, Paola ; Wang, Qi-En ; He, Xiaoming ; Nakano, Ichiro ; Chiocca, E Antonio ; Glorioso Iii, Joseph C ; Kaur, Balveen ; Caligiuri, Michael A ; Yu,...
creationdate20150709
lso0120150709
facets
frbrgroupid8099858529160680572
frbrtype5
newrecords20181218
languageeng
creationdate2015
topic
0Animals–Genetics
1Cell Line, Tumor–Immunology
2Cell Movement–Genetics
3Cytotoxicity, Immunologic–Immunology
4Disease Models, Animal–Metabolism
5Erbb Receptors–Genetics
6Gene Expression–Immunology
7Glioblastoma–Pathology
8Humans–Biosynthesis
9Interferon-Gamma–Immunology
10Killer Cells, Natural–Metabolism...
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Han, Jianfeng
1Chu, Jianhong
2Keung Chan, Wing
3Zhang, Jianying
4Wang, Youwei
5Cohen, Justus B
6Victor, Aaron
7Meisen, Walter H
8Kim, Sung-Hak
9Grandi, Paola
10Wang, Qi-En
11He, Xiaoming
12Nakano, Ichiro
13Chiocca, E Antonio
14Glorioso Iii, Joseph C
15Kaur, Balveen
16Caligiuri, Michael A
17Yu, Jianhua
jtitleScientific reports
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Han
1Chu
2Keung Chan
3Zhang
4Wang
5Cohen
6Victor
7Meisen
8Kim
9Grandi
10He
11Nakano
12Chiocca
13Glorioso Iii
14Kaur
15Caligiuri
16Yu
aufirst
0Jianfeng
1Jianhong
2Wing
3Jianying
4Youwei
5Justus B
6Aaron
7Walter H
8Sung-Hak
9Paola
10Qi-En
11Xiaoming
12Ichiro
13E Antonio
14Joseph C
15Balveen
16Michael A
17Jianhua
au
0Han, Jianfeng
1Chu, Jianhong
2Keung Chan, Wing
3Zhang, Jianying
4Wang, Youwei
5Cohen, Justus B
6Victor, Aaron
7Meisen, Walter H
8Kim, Sung-Hak
9Grandi, Paola
10Wang, Qi-En
11He, Xiaoming
12Nakano, Ichiro
13Chiocca, E Antonio
14Glorioso Iii, Joseph C
15Kaur, Balveen
16Caligiuri, Michael A
17Yu, Jianhua
addauHan, Jianfeng
atitleCAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells.
jtitleScientific reports
risdate20150709
volume5
spage11483
epage11483
pages11483
eissn2045-2322
formatjournal
genrearticle
ristypeJOUR
doi10.1038/srep11483
urlhttp://search.proquest.com/docview/1695182613/
issue1
date2015-07-09