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Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer.

A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of t... Full description

Journal Title: Applied microbiology and biotechnology September 2015, Vol.99(18), pp.7569-7577
Main Author: Li, Ning
Other Authors: Zhao, Lijie , Ng, Tzi Bun , Wong, Jack Ho , Yan, Yan , Shi, Zhong , Liu, Fang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1432-0614 ; DOI: 1432-0614 ; DOI: 10.1007/s00253-015-6499-z
Link: http://search.proquest.com/docview/1706207985/?pq-origsite=primo
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title: Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer.
format: Article
creator:
  • Li, Ning
  • Zhao, Lijie
  • Ng, Tzi Bun
  • Wong, Jack Ho
  • Yan, Yan
  • Shi, Zhong
  • Liu, Fang
subjects:
  • Adsorption–Chemistry
  • Agaricales–Isolation & Purification
  • Antioxidants–Isolation & Purification
  • Free Radical Scavengers–Chemistry
  • Polymers–Isolation & Purification
  • Pyrones–Isolation & Purification
  • Antioxidants
  • Free Radical Scavengers
  • Polymers
  • Pyrones
  • Hispidin
ispartof: Applied microbiology and biotechnology, September 2015, Vol.99(18), pp.7569-7577
description: A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C.sub.18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products. Electronic supplementary material The online version of this article (doi:10.1007/s00253-015-6499-z) contains supplementary material, which is available to authorized users.
language: eng
source:
identifier: E-ISSN: 1432-0614 ; DOI: 1432-0614 ; DOI: 10.1007/s00253-015-6499-z
fulltext: fulltext
issn:
  • 14320614
  • 1432-0614
url: Link


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titleSeparation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer.
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descriptionA new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C.sub.18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products. Electronic supplementary material The online version of this article (doi:10.1007/s00253-015-6499-z) contains supplementary material, which is available to authorized users.
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