schliessen

Filtern

 

Bibliotheken

Fluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.

The membrane skeleton of mature erythrocyte is formed during erythroid differentiation. Fluid shear stress is one of the main factors that promote embryonic hematopoiesis, however, its effects on erythroid differentiation and cytoskeleton remodeling are unclear. Erythrocyte tropomodulin of 41 kDa (E... Full description

Journal Title: PloS one 2015, Vol.10(8), p.e0136607
Main Author: Mu, Weiyun
Other Authors: Wang, Xifu , Zhang, Xiaolan , Zhu, Sida , Sun, Dagong , Ka, Weibo , Sung, Lanping Amy , Yao, Weijuan
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136607
Link: http://search.proquest.com/docview/1708894197/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest1708894197
title: Fluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.
format: Article
creator:
  • Mu, Weiyun
  • Wang, Xifu
  • Zhang, Xiaolan
  • Zhu, Sida
  • Sun, Dagong
  • Ka, Weibo
  • Sung, Lanping Amy
  • Yao, Weijuan
subjects:
  • Actin Cytoskeleton–Metabolism
  • Actins–Metabolism
  • Animals–Cytology
  • Blotting, Western–Metabolism
  • Cell Differentiation–Physiology
  • Cell Proliferation–Genetics
  • Cells, Cultured–Pathology
  • Erythrocytes–Genetics
  • Erythropoiesis–Genetics
  • Female–Genetics
  • Gene Expression Regulation–Metabolism
  • Leukemia, Erythroblastic, Acute–Metabolism
  • Mice–Metabolism
  • Mice, Inbred C57bl–Metabolism
  • Micrornas–Metabolism
  • RNA, Messenger–Metabolism
  • Real-Time Polymerase Chain Reaction–Metabolism
  • Reverse Transcriptase Polymerase Chain Reaction–Metabolism
  • Stress, Mechanical–Metabolism
  • Tropomodulin–Metabolism
  • Actins
  • Micrornas
  • Mirn23b Microrna, Mouse
  • RNA, Messenger
  • Tropomodulin
ispartof: PloS one, 2015, Vol.10(8), p.e0136607
description: The membrane skeleton of mature erythrocyte is formed during erythroid differentiation. Fluid shear stress is one of the main factors that promote embryonic hematopoiesis, however, its effects on erythroid differentiation and cytoskeleton remodeling are unclear. Erythrocyte tropomodulin of 41 kDa (E-Tmod41) caps the pointed end of actin filament (F-actin) and is critical for the formation of hexagonal topology of erythrocyte membrane skeleton. Our study focused on the regulation of E-Tmod41 and its role in F-actin cytoskeleton remodeling during erythroid differentiation induced by fluid shear stress. Mouse erythroleukemia (MEL) cells and embryonic erythroblasts were subjected to fluid shear stress (5 dyn/cm.sup.2) and erythroid differentiation was induced in both cells. F-actin content and E-Tmod41 expression were significantly increased in MEL cells after shearing. E-Tmod41 overexpression resulted in a significant increase in F-actin content, while the knockdown of E-Tmod41 generated the opposite result. An E-Tmod 3'UTR targeting miRNA, miR-23b-3p, was found suppressed by shear stress. When miR-23b-3p level was overexpressed / inhibited, both E-Tmod41 protein level and F-actin content were reduced / augmented. Furthermore, among the two alternative promoters of E-Tmod, P.sub.E0 (upstream of exon 0), which mainly drives the expression of E-Tmod41, was found activated by shear stress. In conclusion, our results suggest that fluid shear stress could induce erythroid differentiation and F-actin cytoskeleton remodeling. It upregulates E-Tmod41 expression through miR-23b-3p suppression and P.sub.E0 promoter activation, which, in turn, contributes to F-actin cytoskeleton remodeling.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136607
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


@attributes
ID867380068
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid1708894197
sourceidproquest
recordidTN_proquest1708894197
sourcesystemPC
pqid1708894197
galeid426725459
display
typearticle
titleFluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.
creatorMu, Weiyun ; Wang, Xifu ; Zhang, Xiaolan ; Zhu, Sida ; Sun, Dagong ; Ka, Weibo ; Sung, Lanping Amy ; Yao, Weijuan
contributorMu, Weiyun (correspondence author) ; Mu, Weiyun (record owner)
ispartofPloS one, 2015, Vol.10(8), p.e0136607
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0136607
subjectActin Cytoskeleton–Metabolism ; Actins–Metabolism ; Animals–Cytology ; Blotting, Western–Metabolism ; Cell Differentiation–Physiology ; Cell Proliferation–Genetics ; Cells, Cultured–Pathology ; Erythrocytes–Genetics ; Erythropoiesis–Genetics ; Female–Genetics ; Gene Expression Regulation–Metabolism ; Leukemia, Erythroblastic, Acute–Metabolism ; Mice–Metabolism ; Mice, Inbred C57bl–Metabolism ; Micrornas–Metabolism ; RNA, Messenger–Metabolism ; Real-Time Polymerase Chain Reaction–Metabolism ; Reverse Transcriptase Polymerase Chain Reaction–Metabolism ; Stress, Mechanical–Metabolism ; Tropomodulin–Metabolism ; Actins ; Micrornas ; Mirn23b Microrna, Mouse ; RNA, Messenger ; Tropomodulin
languageeng
source
descriptionThe membrane skeleton of mature erythrocyte is formed during erythroid differentiation. Fluid shear stress is one of the main factors that promote embryonic hematopoiesis, however, its effects on erythroid differentiation and cytoskeleton remodeling are unclear. Erythrocyte tropomodulin of 41 kDa (E-Tmod41) caps the pointed end of actin filament (F-actin) and is critical for the formation of hexagonal topology of erythrocyte membrane skeleton. Our study focused on the regulation of E-Tmod41 and its role in F-actin cytoskeleton remodeling during erythroid differentiation induced by fluid shear stress. Mouse erythroleukemia (MEL) cells and embryonic erythroblasts were subjected to fluid shear stress (5 dyn/cm.sup.2) and erythroid differentiation was induced in both cells. F-actin content and E-Tmod41 expression were significantly increased in MEL cells after shearing. E-Tmod41 overexpression resulted in a significant increase in F-actin content, while the knockdown of E-Tmod41 generated the opposite result. An E-Tmod 3'UTR targeting miRNA, miR-23b-3p, was found suppressed by shear stress. When miR-23b-3p level was overexpressed / inhibited, both E-Tmod41 protein level and F-actin content were reduced / augmented. Furthermore, among the two alternative promoters of E-Tmod, P.sub.E0 (upstream of exon 0), which mainly drives the expression of E-Tmod41, was found activated by shear stress. In conclusion, our results suggest that fluid shear stress could induce erythroid differentiation and F-actin cytoskeleton remodeling. It upregulates E-Tmod41 expression through miR-23b-3p suppression and P.sub.E0 promoter activation, which, in turn, contributes to F-actin cytoskeleton remodeling.
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/1708894197/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Mu, Weiyun
1Wang, Xifu
2Zhang, Xiaolan
3Zhu, Sida
4Sun, Dagong
5Ka, Weibo
6Sung, Lanping Amy
7Yao, Weijuan
titleFluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.
subject
0Actin Cytoskeleton–Metabolism
1Actins–Metabolism
2Animals–Cytology
3Blotting, Western–Metabolism
4Cell Differentiation–Physiology
5Cell Proliferation–Genetics
6Cells, Cultured–Pathology
7Erythrocytes–Genetics
8Erythropoiesis–Genetics
9Female–Genetics
10Gene Expression Regulation–Metabolism ;...
general
0English
110.1371/journal.pone.0136607
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest1708894197
issn
019326203
11932-6203
rsrctypearticle
creationdate2015
addtitlePloS one
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorMu, Weiyun
startdate20150101
enddate20150101
citationpf e0136607 pt e0136607 vol 10 issue 8
lsr30VSR-Enriched:[galeid, description, pqid]
sort
titleFluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.
authorMu, Weiyun ; Wang, Xifu ; Zhang, Xiaolan ; Zhu, Sida ; Sun, Dagong ; Ka, Weibo ; Sung, Lanping Amy ; Yao, Weijuan
creationdate20150101
lso0120150101
facets
frbrgroupid9167037899142018663
frbrtype5
newrecords20181218
languageeng
creationdate2015
topic
0Actin Cytoskeleton–Metabolism
1Actins–Metabolism
2Animals–Cytology
3Blotting, Western–Metabolism
4Cell Differentiation–Physiology
5Cell Proliferation–Genetics
6Cells, Cultured–Pathology
7Erythrocytes–Genetics
8Erythropoiesis–Genetics
9Female–Genetics
10Gene Expression Regulation–Metabolism ;...
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Mu, Weiyun
1Wang, Xifu
2Zhang, Xiaolan
3Zhu, Sida
4Sun, Dagong
5Ka, Weibo
6Sung, Lanping Amy
7Yao, Weijuan
jtitlePloS one
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Mu
1Wang
2Zhang
3Zhu
4Sun
5Ka
6Sung
7Yao
aufirst
0Weiyun
1Xifu
2Xiaolan
3Sida
4Dagong
5Weibo
6Lanping Amy
7Weijuan
au
0Mu, Weiyun
1Wang, Xifu
2Zhang, Xiaolan
3Zhu, Sida
4Sun, Dagong
5Ka, Weibo
6Sung, Lanping Amy
7Yao, Weijuan
addauMu, Weiyun
atitleFluid Shear Stress Upregulates E-Tmod41 via miR-23b-3p and Contributes to F-Actin Cytoskeleton Remodeling during Erythropoiesis.
jtitlePloS one
risdate20150101
volume10
issue8
spagee0136607
epagee0136607
pagese0136607
eissn1932-6203
formatjournal
genrearticle
ristypeJOUR
doi10.1371/journal.pone.0136607
urlhttp://search.proquest.com/docview/1708894197/
date2015-01-01