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An Apta-Biosensor for Colon Cancer Diagnostics

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are... Full description

Journal Title: Sensors 2015, Vol.15(9), pp.22291-22303
Main Author: Raji, Mojgan
Other Authors: Amoabediny, Ghasem , Tajik, Parviz , Hosseini, Morteza , Ghafar-Zadeh, Ebrahim
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 14248220 ; DOI: 10.3390/s150922291
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title: An Apta-Biosensor for Colon Cancer Diagnostics
format: Article
creator:
  • Raji, Mojgan
  • Amoabediny, Ghasem
  • Tajik, Parviz
  • Hosseini, Morteza
  • Ghafar-Zadeh, Ebrahim
subjects:
  • Aptamer
  • Aptasensor
  • Biosensor
  • Colon Cancer
  • Cyclic Voltammetry (Cv)
  • Fluorescence Labeling
  • Aptamers, Nucleotide -- Chemistry
  • Biosensing Techniques -- Methods
  • Colonic Neoplasms -- Diagnosis
ispartof: Sensors, 2015, Vol.15(9), pp.22291-22303
description: This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
language: eng
source:
identifier: E-ISSN: 14248220 ; DOI: 10.3390/s150922291
fulltext: fulltext_linktorsrc
issn:
  • 14248220
  • 1424-8220
url: Link


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titleAn Apta-Biosensor for Colon Cancer Diagnostics
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descriptionThis paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
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subjectAptamer ; Aptasensor ; Biosensor ; Colon Cancer ; Cyclic Voltammetry (Cv) ; Fluorescence Labeling ; Aptamers, Nucleotide -- Chemistry ; Biosensing Techniques -- Methods ; Colonic Neoplasms -- Diagnosis;
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titleAn Apta-Biosensor for Colon Cancer Diagnostics
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abstractThis paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.
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