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Programmed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid.

Honey bees are at an unavoidable risk of exposure to neonicotinoid pesticides, which are used worldwide. Compared with the well-studied roles of these pesticides in nontarget site (including midgut, ovary, or salivary glands), little has been reported in the target sites, the brain. In the current s... Full description

Journal Title: Journal of economic entomology August 2015, Vol.108(4), pp.1486-1494
Main Author: Wu, Yan-Yan
Other Authors: Zhou, Ting , Wang, Qiang , Dai, Ping-Li , Xu, Shu-Fa , Jia, Hui-Ru , Wang, Xing
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0022-0493 ; DOI: 10.1093/jee/tov146
Link: http://search.proquest.com/docview/1722932190/?pq-origsite=primo
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title: Programmed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid.
format: Article
creator:
  • Wu, Yan-Yan
  • Zhou, Ting
  • Wang, Qiang
  • Dai, Ping-Li
  • Xu, Shu-Fa
  • Jia, Hui-Ru
  • Wang, Xing
subjects:
  • Animals–Drug Effects
  • Apoptosis–Drug Effects
  • Autophagy–Physiology
  • Bees–Ultrastructure
  • Brain–Drug Effects
  • Caspase 1–Physiology
  • Dose-Response Relationship, Drug–Ultrastructure
  • Imidazoles–Genetics
  • In Situ Nick-End Labeling–Metabolism
  • Insect Proteins–Toxicity
  • Insecticides–Genetics
  • Microscopy, Electron, Transmission–Metabolism
  • Neonicotinoids–Toxicity
  • Neurons–Drug Effects
  • Nitro Compounds–Physiology
  • RNA, Messenger–Ultrastructure
  • Real-Time Polymerase Chain Reaction–Toxicity
  • Real-Time Polymerase Chain Reaction–Genetics
  • Real-Time Polymerase Chain Reaction–Metabolism
  • Imidazoles
ispartof: Journal of economic entomology, August 2015, Vol.108(4), pp.1486-1494
description: Honey bees are at an unavoidable risk of exposure to neonicotinoid pesticides, which are used worldwide. Compared with the well-studied roles of these pesticides in nontarget site (including midgut, ovary, or salivary glands), little has been reported in the target sites, the brain. In the current study, laboratory-reared adult worker honey bees (Apis mellifera L.) were treated with sublethal doses of imidacloprid. Neuronal apoptosis was detected using the TUNEL technique for DNA labeling. We observed significantly increased apoptotic markers in dose- and time-dependent manners in brains of bees exposed to imidacloprid. Neuronal activated caspase-3 and mRNA levels of caspase-1, as detected by immunofluorescence and real-time quantitative PCR, respectively, were significantly increased, suggesting that sublethal doses of imidacloprid may induce the caspase-dependent apoptotic pathway. Additionally, the overlap of apoptosis and autophagy in neurons was confirmed by transmission electron microscopy. It further suggests that a relationship exists between neurotoxicity and behavioral changes induced by sublethal doses of imidacloprid, and that there is a need to determine reasonable limits for imidacloprid application in the field to protect pollinators. ; p. 1486-1494.
language: eng
source:
identifier: ISSN: 0022-0493 ; DOI: 10.1093/jee/tov146
fulltext: fulltext
issn:
  • 00220493
  • 0022-0493
url: Link


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titleProgrammed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid.
creatorWu, Yan-Yan ; Zhou, Ting ; Wang, Qiang ; Dai, Ping-Li ; Xu, Shu-Fa ; Jia, Hui-Ru ; Wang, Xing
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ispartofJournal of economic entomology, August 2015, Vol.108(4), pp.1486-1494
identifierISSN: 0022-0493 ; DOI: 10.1093/jee/tov146
subjectAnimals–Drug Effects ; Apoptosis–Drug Effects ; Autophagy–Physiology ; Bees–Ultrastructure ; Brain–Drug Effects ; Caspase 1–Physiology ; Dose-Response Relationship, Drug–Ultrastructure ; Imidazoles–Genetics ; In Situ Nick-End Labeling–Metabolism ; Insect Proteins–Toxicity ; Insecticides–Genetics ; Microscopy, Electron, Transmission–Metabolism ; Neonicotinoids–Toxicity ; Neurons–Drug Effects ; Nitro Compounds–Physiology ; RNA, Messenger–Ultrastructure ; Real-Time Polymerase Chain Reaction–Toxicity ; Real-Time Polymerase Chain Reaction–Genetics ; Real-Time Polymerase Chain Reaction–Metabolism ; Imidazoles
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descriptionHoney bees are at an unavoidable risk of exposure to neonicotinoid pesticides, which are used worldwide. Compared with the well-studied roles of these pesticides in nontarget site (including midgut, ovary, or salivary glands), little has been reported in the target sites, the brain. In the current study, laboratory-reared adult worker honey bees (Apis mellifera L.) were treated with sublethal doses of imidacloprid. Neuronal apoptosis was detected using the TUNEL technique for DNA labeling. We observed significantly increased apoptotic markers in dose- and time-dependent manners in brains of bees exposed to imidacloprid. Neuronal activated caspase-3 and mRNA levels of caspase-1, as detected by immunofluorescence and real-time quantitative PCR, respectively, were significantly increased, suggesting that sublethal doses of imidacloprid may induce the caspase-dependent apoptotic pathway. Additionally, the overlap of apoptosis and autophagy in neurons was confirmed by transmission electron microscopy. It further suggests that a relationship exists between neurotoxicity and behavioral changes induced by sublethal doses of imidacloprid, and that there is a need to determine reasonable limits for imidacloprid application in the field to protect pollinators. ; p. 1486-1494.
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titleProgrammed Cell Death in the Honey Bee (Apis mellifera) (Hymenoptera: Apidae) Worker Brain Induced by Imidacloprid.
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