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Metabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach

Ceramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell via... Full description

Journal Title: PLoS One Nov 2015, Vol.10(11), p.e0143385
Main Author: Kjellberg, Matti
Other Authors: Lönnfors, Max , Slotte, J , Mattjus, Peter
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0143385
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title: Metabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach
format: Article
creator:
  • Kjellberg, Matti
  • Lönnfors, Max
  • Slotte, J
  • Mattjus, Peter
subjects:
  • United States–Us
  • Finland
  • Membranes
  • Conversion
  • Phosphocholine
  • Viability
  • Apoptosis
  • Metabolism
  • Biochemistry
  • Precursors
  • Chains
  • Alcohol
  • Engineering
  • Metabolism
  • Dosage
  • Metabolism
  • Lipids
  • Apoptosis
  • Cell Growth
ispartof: PLoS One, Nov 2015, Vol.10(11), p.e0143385
description: Ceramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell viability. We found that all three ceramides were successfully taken up by HeLa cells when complexed to CholPC in an equimolar ratio, and show that the ceramides show different rates of cellular uptake and metabolic fate. The C6-Cer had the highest incorporation rate, followed by C10-Cer and C16-Cer, respectively. The subsequent effect on cell viability strongly correlated with the rate of incorporation, where C6-Cer had the strongest apoptotic effects. Low-dose (1 μM) treatment with C6-Cer favoured conversion of the precursor to sphingomyelin, whereas higher concentrations (25–100 μM) yielded increased conversion to C6-glucosylceramide. Similar results were obtained for C10-Cer. In the lower-dose...
language: eng
source:
identifier: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0143385
fulltext: fulltext_linktorsrc
issn:
  • 19326203
  • 1932-6203
url: Link


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titleMetabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach
creatorKjellberg, Matti ; Lönnfors, Max ; Slotte, J ; Mattjus, Peter
ispartofPLoS One, Nov 2015, Vol.10(11), p.e0143385
identifierE-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0143385
subjectUnited States–Us ; Finland ; Membranes ; Conversion ; Phosphocholine ; Viability ; Apoptosis ; Metabolism ; Biochemistry ; Precursors ; Chains ; Alcohol ; Engineering ; Metabolism ; Dosage ; Metabolism ; Lipids ; Apoptosis ; Cell Growth
descriptionCeramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell viability. We found that all three ceramides were successfully taken up by HeLa cells when complexed to CholPC in an equimolar ratio, and show that the ceramides show different rates of cellular uptake and metabolic fate. The C6-Cer had the highest incorporation rate, followed by C10-Cer and C16-Cer, respectively. The subsequent effect on cell viability strongly correlated with the rate of incorporation, where C6-Cer had the strongest apoptotic effects. Low-dose (1 μM) treatment with C6-Cer favoured conversion of the precursor to sphingomyelin, whereas higher concentrations (25–100 μM) yielded increased conversion to C6-glucosylceramide. Similar results were obtained for C10-Cer. In the lower-dose...
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titleMetabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach
descriptionCeramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell viability. We found that all three ceramides were successfully taken up by HeLa cells when complexed to CholPC in an equimolar ratio, and show that the ceramides show different rates of cellular uptake and metabolic fate. The C6-Cer had the highest incorporation rate, followed by C10-Cer and C16-Cer, respectively. The subsequent effect on cell viability strongly correlated with the rate of incorporation, where C6-Cer had the strongest apoptotic effects. Low-dose (1 μM) treatment with C6-Cer favoured conversion of the precursor to sphingomyelin, whereas higher concentrations (25–100 μM) yielded increased conversion to C6-glucosylceramide. Similar results were obtained for C10-Cer. In the lower-dose...
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citationpf e0143385 vol 10 issue 11
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titleMetabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach
authorKjellberg, Matti ; Lönnfors, Max ; Slotte, J ; Mattjus, Peter
creationdate20151101
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11Alcohol
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abstractCeramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell viability. We found that all three ceramides were successfully taken up by HeLa cells when complexed to CholPC in an equimolar ratio, and show that the ceramides show different rates of cellular uptake and metabolic fate. The C6-Cer had the highest incorporation rate, followed by C10-Cer and C16-Cer, respectively. The subsequent effect on cell viability strongly correlated with the rate of incorporation, where C6-Cer had the strongest apoptotic effects. Low-dose (1 μM) treatment with C6-Cer favoured conversion of the precursor to sphingomyelin, whereas higher concentrations (25–100 μM) yielded increased conversion to C6-glucosylceramide. Similar results were obtained for C10-Cer. In the lower-dose...
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doi10.1371/journal.pone.0143385
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date2015-11-01