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The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco

Plastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding proteas... Full description

Journal Title: Planta Nov 2004, Vol.220(1), pp.97-104
Main Author: Kato, Y
Other Authors: Murakami, S , Yamamoto, Y , Chatani, H , Kondo, Y , Nakano, T , Yokota, A , Sato, F
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0032-0935 ; DOI: 10.1007/s00425-004-1328-0
Link: http://search.proquest.com/docview/17744151/?pq-origsite=primo
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title: The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco
format: Article
creator:
  • Kato, Y
  • Murakami, S
  • Yamamoto, Y
  • Chatani, H
  • Kondo, Y
  • Nakano, T
  • Yokota, A
  • Sato, F
subjects:
  • Nicotiana Tabacum
  • Crops
  • Proteinase
  • Senescence
  • Leaves
  • Nitrogen
  • Nucleoids
  • Dicotyledons (Crops)
  • Ribulose-1,5-Bisphosphate Carboxylase
  • Ribulose-1,5-Bisphosphate Oxygenase
  • Cnd41 Protein
  • Tobacco
  • Cnd41 Protein
  • Ribulose-1,5-Bisphosphate Carboxylase
  • Ribulose-1,5-Bisphosphate Oxygenase
  • Tobacco
ispartof: Planta, Nov 2004, Vol.220(1), pp.97-104
description: Plastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wild-type tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation...
language: eng
source:
identifier: ISSN: 0032-0935 ; DOI: 10.1007/s00425-004-1328-0
fulltext: fulltext
issn:
  • 00320935
  • 0032-0935
url: Link


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titleThe DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco
creatorKato, Y ; Murakami, S ; Yamamoto, Y ; Chatani, H ; Kondo, Y ; Nakano, T ; Yokota, A ; Sato, F
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ispartofPlanta, Nov 2004, Vol.220(1), pp.97-104
identifierISSN: 0032-0935 ; DOI: 10.1007/s00425-004-1328-0
subjectNicotiana Tabacum ; Crops ; Proteinase ; Senescence ; Leaves ; Nitrogen ; Nucleoids ; Dicotyledons (Crops) ; Ribulose-1,5-Bisphosphate Carboxylase ; Ribulose-1,5-Bisphosphate Oxygenase ; Cnd41 Protein ; Tobacco ; Cnd41 Protein ; Ribulose-1,5-Bisphosphate Carboxylase ; Ribulose-1,5-Bisphosphate Oxygenase ; Tobacco
descriptionPlastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wild-type tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation...
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titleThe DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco
descriptionPlastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wild-type tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation...
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titleThe DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco
authorKato, Y ; Murakami, S ; Yamamoto, Y ; Chatani, H ; Kondo, Y ; Nakano, T ; Yokota, A ; Sato, F
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abstractPlastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wild-type tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation...
doi10.1007/s00425-004-1328-0
urlhttp://search.proquest.com/docview/17744151/
eissn14322048
date2004-11-01