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AB0097 Th17 Cytokines Regulate Osteoclastogenesis in Rheumatoid Arthritis

Background Th17 cells, which are defined by a selective interleukin (IL)-17 secretion, are involved in the bony destruction of rheumatoid arthritis (RA). Objectives This study aimed to determine the regulatory effect of Th17 cytokines on the osteoclastogenesis in RA. Methods The expression of IL-17... Full description

Journal Title: Annals of the Rheumatic Diseases Jun 2014, Vol.73, p.835
Main Author: Kim, Hr
Other Authors: Lee, S-H
Format: Electronic Article Electronic Article
Language: English
ID: ISSN: 00034967 ; E-ISSN: 14682060 ; DOI: 10.1136/annrheumdis-2014-eular.1977
Link: http://search.proquest.com/docview/1777977646/?pq-origsite=primo
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title: AB0097 Th17 Cytokines Regulate Osteoclastogenesis in Rheumatoid Arthritis
format: Article
creator:
  • Kim, Hr
  • Lee, S-H
ispartof: Annals of the Rheumatic Diseases, Jun 2014, Vol.73, p.835
description: Background Th17 cells, which are defined by a selective interleukin (IL)-17 secretion, are involved in the bony destruction of rheumatoid arthritis (RA). Objectives This study aimed to determine the regulatory effect of Th17 cytokines on the osteoclastogenesis in RA. Methods The expression of IL-17 and RANKL was determined in synovial tissue, fibroblast-like synoviocytes (FLS) and synovial fluids of RA patients using immonohistochemical staining, ELISA and real-time PCR. The Th17 cytokines-induced RANKL expression was studied in RA FLS using real-time PCR, luciferase activity, and western blot. Human peripheral blood monocytes were cultured with M-CSF and Th17 cytokines, and then osteoclastogenesis was determined by counting the number TRAP-positive multinucleated cells. The osteoclastogenesis was also determined after human monocytes were co-cultured with IL-17-prestimulated FLS. Results There was significant correlation between RANKL and IL-17 levels in RA synovial fluid. After RA FLS...
language: eng
source:
identifier: ISSN: 00034967 ; E-ISSN: 14682060 ; DOI: 10.1136/annrheumdis-2014-eular.1977
fulltext: fulltext
issn:
  • 00034967
  • 0003-4967
  • 14682060
  • 1468-2060
url: Link


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descriptionBackground Th17 cells, which are defined by a selective interleukin (IL)-17 secretion, are involved in the bony destruction of rheumatoid arthritis (RA). Objectives This study aimed to determine the regulatory effect of Th17 cytokines on the osteoclastogenesis in RA. Methods The expression of IL-17 and RANKL was determined in synovial tissue, fibroblast-like synoviocytes (FLS) and synovial fluids of RA patients using immonohistochemical staining, ELISA and real-time PCR. The Th17 cytokines-induced RANKL expression was studied in RA FLS using real-time PCR, luciferase activity, and western blot. Human peripheral blood monocytes were cultured with M-CSF and Th17 cytokines, and then osteoclastogenesis was determined by counting the number TRAP-positive multinucleated cells. The osteoclastogenesis was also determined after human monocytes were co-cultured with IL-17-prestimulated FLS. Results There was significant correlation between RANKL and IL-17 levels in RA synovial fluid. After RA FLS...
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descriptionBackground Th17 cells, which are defined by a selective interleukin (IL)-17 secretion, are involved in the bony destruction of rheumatoid arthritis (RA). Objectives This study aimed to determine the regulatory effect of Th17 cytokines on the osteoclastogenesis in RA. Methods The expression of IL-17 and RANKL was determined in synovial tissue, fibroblast-like synoviocytes (FLS) and synovial fluids of RA patients using immonohistochemical staining, ELISA and real-time PCR. The Th17 cytokines-induced RANKL expression was studied in RA FLS using real-time PCR, luciferase activity, and western blot. Human peripheral blood monocytes were cultured with M-CSF and Th17 cytokines, and then osteoclastogenesis was determined by counting the number TRAP-positive multinucleated cells. The osteoclastogenesis was also determined after human monocytes were co-cultured with IL-17-prestimulated FLS. Results There was significant correlation between RANKL and IL-17 levels in RA synovial fluid. After RA FLS...
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abstractBackground Th17 cells, which are defined by a selective interleukin (IL)-17 secretion, are involved in the bony destruction of rheumatoid arthritis (RA). Objectives This study aimed to determine the regulatory effect of Th17 cytokines on the osteoclastogenesis in RA. Methods The expression of IL-17 and RANKL was determined in synovial tissue, fibroblast-like synoviocytes (FLS) and synovial fluids of RA patients using immonohistochemical staining, ELISA and real-time PCR. The Th17 cytokines-induced RANKL expression was studied in RA FLS using real-time PCR, luciferase activity, and western blot. Human peripheral blood monocytes were cultured with M-CSF and Th17 cytokines, and then osteoclastogenesis was determined by counting the number TRAP-positive multinucleated cells. The osteoclastogenesis was also determined after human monocytes were co-cultured with IL-17-prestimulated FLS. Results There was significant correlation between RANKL and IL-17 levels in RA synovial fluid. After RA FLS...
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date2014-06-01