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Chemotaxis for enhanced immobilization of Escherichia coli and Legionella pneumophila on biofunctionalized surfaces of GaAs.

The authors have investigated the effect of chemotaxis on immobilization of bacteria on the surface of biofunctionalized GaAs (001) samples. Escherichia coli K12 bacteria were employed to provide a proof-of-concept of chemotaxis-enhanced bacterial immobilization, and then, these results were confirm... Full description

Journal Title: Biointerphases June 20, 2016, Vol.11(2), p.021004
Main Author: Hassen, Walid M
Other Authors: Sanyal, Hashimita , Hammood, Manar , Moumanis, Khalid , Frost, Eric H , Dubowski, Jan J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1559-4106 ; DOI: 10.1116/1.4947048
Link: http://search.proquest.com/docview/1783921347/?pq-origsite=primo
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recordid: proquest1783921347
title: Chemotaxis for enhanced immobilization of Escherichia coli and Legionella pneumophila on biofunctionalized surfaces of GaAs.
format: Article
creator:
  • Hassen, Walid M
  • Sanyal, Hashimita
  • Hammood, Manar
  • Moumanis, Khalid
  • Frost, Eric H
  • Dubowski, Jan J
subjects:
  • Antibodies, Bacterial–Metabolism
  • Arsenicals–Physiology
  • Bacterial Adhesion–Metabolism
  • Cells, Immobilized–Drug Effects
  • Chemotaxis–Physiology
  • Enzymes, Immobilized–Metabolism
  • Escherichia Coli K12–Drug Effects
  • Gallium–Physiology
  • Glucose–Metabolism
  • Legionella Pneumophila–Metabolism
  • Beta-Galactosidase–Metabolism
  • Antibodies, Bacterial
  • Arsenicals
  • Enzymes, Immobilized
  • Gallium Arsenide
  • Gallium
  • Beta-Galactosidase
  • Glucose
ispartof: Biointerphases, June 20, 2016, Vol.11(2), p.021004
description: The authors have investigated the effect of chemotaxis on immobilization of bacteria on the surface of biofunctionalized GaAs (001) samples. Escherichia coli K12 bacteria were employed to provide a proof-of-concept of chemotaxis-enhanced bacterial immobilization, and then, these results were confirmed using Legionella pneumophila. The recognition layer was based on a self-assembled monolayer of thiol functionalized with specific antibodies directed toward E. coli or L. pneumophila, together with the enzyme beta-galactosidase (β-gal). The authors hypothesized that this enzyme together with its substrate lactose would produce a gradient of glucose which would attract bacteria toward the biochip surface. The chemotaxis effect was monitored by comparing the number of bacteria bound to the biochip surface with and without attractant. The authors have observed that β-gal plus lactose enhanced the immobilization of bacteria on our biochips with a higher effect at low bacterial concentrations....
language: eng
source:
identifier: E-ISSN: 1559-4106 ; DOI: 10.1116/1.4947048
fulltext: fulltext
issn:
  • 15594106
  • 1559-4106
url: Link


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titleChemotaxis for enhanced immobilization of Escherichia coli and Legionella pneumophila on biofunctionalized surfaces of GaAs.
creatorHassen, Walid M ; Sanyal, Hashimita ; Hammood, Manar ; Moumanis, Khalid ; Frost, Eric H ; Dubowski, Jan J
contributorHassen, Walid M (correspondence author) ; Hassen, Walid M (record owner)
ispartofBiointerphases, June 20, 2016, Vol.11(2), p.021004
identifierE-ISSN: 1559-4106 ; DOI: 10.1116/1.4947048
subjectAntibodies, Bacterial–Metabolism ; Arsenicals–Physiology ; Bacterial Adhesion–Metabolism ; Cells, Immobilized–Drug Effects ; Chemotaxis–Physiology ; Enzymes, Immobilized–Metabolism ; Escherichia Coli K12–Drug Effects ; Gallium–Physiology ; Glucose–Metabolism ; Legionella Pneumophila–Metabolism ; Beta-Galactosidase–Metabolism ; Antibodies, Bacterial ; Arsenicals ; Enzymes, Immobilized ; Gallium Arsenide ; Gallium ; Beta-Galactosidase ; Glucose
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descriptionThe authors have investigated the effect of chemotaxis on immobilization of bacteria on the surface of biofunctionalized GaAs (001) samples. Escherichia coli K12 bacteria were employed to provide a proof-of-concept of chemotaxis-enhanced bacterial immobilization, and then, these results were confirmed using Legionella pneumophila. The recognition layer was based on a self-assembled monolayer of thiol functionalized with specific antibodies directed toward E. coli or L. pneumophila, together with the enzyme beta-galactosidase (β-gal). The authors hypothesized that this enzyme together with its substrate lactose would produce a gradient of glucose which would attract bacteria toward the biochip surface. The chemotaxis effect was monitored by comparing the number of bacteria bound to the biochip surface with and without attractant. The authors have observed that β-gal plus lactose enhanced the immobilization of bacteria on our biochips with a higher effect at low bacterial concentrations....
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authorHassen, Walid M ; Sanyal, Hashimita ; Hammood, Manar ; Moumanis, Khalid ; Frost, Eric H ; Dubowski, Jan J
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