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Immunostimulatory CpG motifs induce CTL responses to HIV type I oligomeric gp140 envelope protein

SummaryIn the present study we investigated the immunomodulatory effects of two adjuvants, liposomal lipid A [L(LA)] and CpG-containing oligodeoxynucleotides (CpG ODN), to the HIV-1 ogp140 envelope protein. Administration of each of these adjuvants separately with unencapsulated ogp140 resulted in l... Full description

Journal Title: Immunology and Cell Biology Oct 2004, Vol.82(5), pp.523-530
Main Author: Rao, Mangala
Other Authors: Matyas, Gary , Vancott, Thomas , Birx, Deborah , Alving, Carl
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 08189641 ; E-ISSN: 14401711 ; DOI: 10.1111/j.0818-9641.2004.01283.x
Link: http://search.proquest.com/docview/1786820780/?pq-origsite=primo
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title: Immunostimulatory CpG motifs induce CTL responses to HIV type I oligomeric gp140 envelope protein
format: Article
creator:
  • Rao, Mangala
  • Matyas, Gary
  • Vancott, Thomas
  • Birx, Deborah
  • Alving, Carl
subjects:
  • Adjuvants, Immunologic, Pharmacology
  • Cpg Islands, Immunology
  • Gene Products, Env, Immunology
  • Hiv-1, Immunology
  • T-Lymphocytes, Cytotoxic, Immunology
  • Adjuvants, Immunologic, Administration And Dosage
  • Animals
  • Antibody Formation, Drug Effects
  • Dimerization
  • Female
  • Hiv-1, Chemistry
  • Lipid A, Administration And Dosage
  • Lipid A, Pharmacology
  • Liposomes, Administration And Dosage
  • Liposomes, Pharmacology
  • Mice
  • Mice, Inbred Balb C
  • Oligodeoxyribonucleotides, Administration And Dosage
  • Oligodeoxyribonucleotides, Pharmacology
  • Th1 Cells, Drug Effects
  • Th2 Cells, Drug Effects
ispartof: Immunology and Cell Biology, Oct 2004, Vol.82(5), pp.523-530
description: SummaryIn the present study we investigated the immunomodulatory effects of two adjuvants, liposomal lipid A [L(LA)] and CpG-containing oligodeoxynucleotides (CpG ODN), to the HIV-1 ogp140 envelope protein. Administration of each of these adjuvants separately with unencapsulated ogp140 resulted in low antibody titres. Encapsulation of ogp140 in liposomes containing lipid A resulted in a sixfold increase in anti-ogp140 antibodies. The antibody titres were further enhanced threefold by the addition of CpG ODN. Priming and boosting BALB/c mice with unencapsulated ogp140 with L(LA) or encapsulation in liposomes containing lipid A induced a mixed Th1/Th2 type of immune response. In contrast, immunization with L(ogp140 + LA) plus CpG ODN switched the immune response to a Th-1 response with elevated anti-ogp140 IgG2a antibodies and IFN-γ levels. Both adjuvants induced excellent ogp140-specific proliferative and CTL responses. Therefore, for the induction of high titre antibodies, but not for cellular responses, the antigen and lipid A have to be present in the same liposomes. These results can have significant implications in directing the Th1 or Th2 differentiation of antigen-specific immune responses in the context of vaccine development.
language: eng
source:
identifier: ISSN: 08189641 ; E-ISSN: 14401711 ; DOI: 10.1111/j.0818-9641.2004.01283.x
fulltext: fulltext
issn:
  • 08189641
  • 0818-9641
  • 14401711
  • 1440-1711
url: Link


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titleImmunostimulatory CpG motifs induce CTL responses to HIV type I oligomeric gp140 envelope protein
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ispartofImmunology and Cell Biology, Oct 2004, Vol.82(5), pp.523-530
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descriptionSummaryIn the present study we investigated the immunomodulatory effects of two adjuvants, liposomal lipid A [L(LA)] and CpG-containing oligodeoxynucleotides (CpG ODN), to the HIV-1 ogp140 envelope protein. Administration of each of these adjuvants separately with unencapsulated ogp140 resulted in low antibody titres. Encapsulation of ogp140 in liposomes containing lipid A resulted in a sixfold increase in anti-ogp140 antibodies. The antibody titres were further enhanced threefold by the addition of CpG ODN. Priming and boosting BALB/c mice with unencapsulated ogp140 with L(LA) or encapsulation in liposomes containing lipid A induced a mixed Th1/Th2 type of immune response. In contrast, immunization with L(ogp140 + LA) plus CpG ODN switched the immune response to a Th-1 response with elevated anti-ogp140 IgG2a antibodies and IFN-γ levels. Both adjuvants induced excellent ogp140-specific proliferative and CTL responses. Therefore, for the induction of high titre antibodies, but not for cellular responses, the antigen and lipid A have to be present in the same liposomes. These results can have significant implications in directing the Th1 or Th2 differentiation of antigen-specific immune responses in the context of vaccine development.
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subjectAdjuvants, Immunologic, Pharmacology ; Cpg Islands, Immunology ; Gene Products, Env, Immunology ; Hiv-1, Immunology ; T-Lymphocytes, Cytotoxic, Immunology ; Adjuvants, Immunologic, Administration And Dosage ; Animals ; Antibody Formation, Drug Effects ; Dimerization ; Female ; Hiv-1, Chemistry ; Lipid A, Administration And Dosage ; Lipid A, Pharmacology ; Liposomes, Administration And Dosage ; Liposomes, Pharmacology ; Mice ; Mice, Inbred Balb C ; Oligodeoxyribonucleotides, Administration And Dosage ; Oligodeoxyribonucleotides, Pharmacology ; Th1 Cells, Drug Effects ; Th2 Cells, Drug Effects;
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abstractSummaryIn the present study we investigated the immunomodulatory effects of two adjuvants, liposomal lipid A [L(LA)] and CpG-containing oligodeoxynucleotides (CpG ODN), to the HIV-1 ogp140 envelope protein. Administration of each of these adjuvants separately with unencapsulated ogp140 resulted in low antibody titres. Encapsulation of ogp140 in liposomes containing lipid A resulted in a sixfold increase in anti-ogp140 antibodies. The antibody titres were further enhanced threefold by the addition of CpG ODN. Priming and boosting BALB/c mice with unencapsulated ogp140 with L(LA) or encapsulation in liposomes containing lipid A induced a mixed Th1/Th2 type of immune response. In contrast, immunization with L(ogp140 + LA) plus CpG ODN switched the immune response to a Th-1 response with elevated anti-ogp140 IgG2a antibodies and IFN-γ levels. Both adjuvants induced excellent ogp140-specific proliferative and CTL responses. Therefore, for the induction of high titre antibodies, but not for cellular responses, the antigen and lipid A have to be present in the same liposomes. These results can have significant implications in directing the Th1 or Th2 differentiation of antigen-specific immune responses in the context of vaccine development.
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pubBlackwell Science Ltd.
doi10.1111/j.0818-9641.2004.01283.x
urlhttp://search.proquest.com/docview/1786820780/
date2004-10-01