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Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes

Axin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocyt... Full description

Journal Title: PLoS One Jun 2016, Vol.11(6), p.e0157197
Main Author: Xiao-Qin, He
Other Authors: Yue-Qiang, Song , Liu, Rui , Liu, Yu , Zhang, Fei , Zhang, Zhen , Yu-Ting, Shen , Xu, Lin , Ming-Huang, Chen , Ya-Long, Wang , Bai-Hui, Xu , Xiang-Jun, Yang , Hai-Long, Wang
Format: Electronic Article Electronic Article
Language: English
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ID: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0157197
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title: Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes
format: Article
creator:
  • Xiao-Qin, He
  • Yue-Qiang, Song
  • Liu, Rui
  • Liu, Yu
  • Zhang, Fei
  • Zhang, Zhen
  • Yu-Ting, Shen
  • Xu, Lin
  • Ming-Huang, Chen
  • Ya-Long, Wang
  • Bai-Hui, Xu
  • Xiang-Jun, Yang
  • Hai-Long, Wang
subjects:
  • Assembly
  • Kinetochores
  • Wnt Protein
  • Chromosomes
  • Spindles
  • Cell Culture
  • Axin1 Gene
  • Lithium Chloride
  • Bubr1 Protein
  • Tubulin
  • Localization
  • Lithium Chlorides
  • Oogenesis
  • Maturation
  • Chromosomes
  • Microinjection
  • Chromosomes
  • Mitosis
  • Gene Expression
  • Immunofluorescence
ispartof: PLoS One, Jun 2016, Vol.11(6), p.e0157197
description: Axin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle. Knockdown of the Axin1 gene by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in severely defective spindles, misaligned chromosomes, failure of first polar body (PB1) extrusion, and impaired pronuclear formation. However, supplementing the culture medium with the Wnt pathway activator LiCl improved spindle morphology and pronuclear formation. Downregulation of Axin1 gene expression also impaired the spindle pole localization of γ-tubulin/Nek9 and resulted in retention of the spindle assembly checkpoint protein BubR1 at kinetochores after 8.5 h of culture. Our results...
language: eng
source:
identifier: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0157197
fulltext: fulltext_linktorsrc
issn:
  • 19326203
  • 1932-6203
url: Link


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titleAxin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes
creatorXiao-Qin, He ; Yue-Qiang, Song ; Liu, Rui ; Liu, Yu ; Zhang, Fei ; Zhang, Zhen ; Yu-Ting, Shen ; Xu, Lin ; Ming-Huang, Chen ; Ya-Long, Wang ; Bai-Hui, Xu ; Xiang-Jun, Yang ; Hai-Long, Wang
ispartofPLoS One, Jun 2016, Vol.11(6), p.e0157197
identifierE-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0157197
subjectAssembly ; Kinetochores ; Wnt Protein ; Chromosomes ; Spindles ; Cell Culture ; Axin1 Gene ; Lithium Chloride ; Bubr1 Protein ; Tubulin ; Localization ; Lithium Chlorides ; Oogenesis ; Maturation ; Chromosomes ; Microinjection ; Chromosomes ; Mitosis ; Gene Expression ; Immunofluorescence
descriptionAxin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle. Knockdown of the Axin1 gene by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in severely defective spindles, misaligned chromosomes, failure of first polar body (PB1) extrusion, and impaired pronuclear formation. However, supplementing the culture medium with the Wnt pathway activator LiCl improved spindle morphology and pronuclear formation. Downregulation of Axin1 gene expression also impaired the spindle pole localization of γ-tubulin/Nek9 and resulted in retention of the spindle assembly checkpoint protein BubR1 at kinetochores after 8.5 h of culture. Our results...
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titleAxin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes
descriptionAxin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle. Knockdown of the Axin1 gene by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in severely defective spindles, misaligned chromosomes, failure of first polar body (PB1) extrusion, and impaired pronuclear formation. However, supplementing the culture medium with the Wnt pathway activator LiCl improved spindle morphology and pronuclear formation. Downregulation of Axin1 gene expression also impaired the spindle pole localization of γ-tubulin/Nek9 and resulted in retention of the spindle assembly checkpoint protein BubR1 at kinetochores after 8.5 h of culture. Our results...
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titleAxin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes
authorXiao-Qin, He ; Yue-Qiang, Song ; Liu, Rui ; Liu, Yu ; Zhang, Fei ; Zhang, Zhen ; Yu-Ting, Shen ; Xu, Lin ; Ming-Huang, Chen ; Ya-Long, Wang ; Bai-Hui, Xu ; Xiang-Jun, Yang ; Hai-Long, Wang
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1Kinetochores
2Wnt Protein
3Chromosomes
4Spindles
5Cell Culture
6Axin1 Gene
7Lithium Chloride
8Bubr1 Protein
9Tubulin
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13Maturation
14Microinjection
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16Gene Expression
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abstractAxin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown. Here we examined the localization and function of Axin-1 during meiotic maturation in mouse oocytes. Immunofluorescence analysis showed that Axin-1 was localized around the spindle. Knockdown of the Axin1 gene by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in severely defective spindles, misaligned chromosomes, failure of first polar body (PB1) extrusion, and impaired pronuclear formation. However, supplementing the culture medium with the Wnt pathway activator LiCl improved spindle morphology and pronuclear formation. Downregulation of Axin1 gene expression also impaired the spindle pole localization of γ-tubulin/Nek9 and resulted in retention of the spindle assembly checkpoint protein BubR1 at kinetochores after 8.5 h of culture. Our results...
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