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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Ditching Invading DNABacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas). Jinek et al. (p. 816, published online 28 June; see the P... Full description

Journal Title: Science August 17, 2012, Vol.337(6096), pp.816-821
Main Author: Jinek, Martin
Other Authors: Chylinski, Krzysztof , Fonfara, Ines , Hauer, Michael , Doudna, Jennifer , Charpentier, Emmanuelle
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0036-8075 ; DOI: 10.1126/science.1225829
Link: http://search.proquest.com/docview/1808090978/?pq-origsite=primo
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recordid: proquest1808090978
title: A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
format: Article
creator:
  • Jinek, Martin
  • Chylinski, Krzysztof
  • Fonfara, Ines
  • Hauer, Michael
  • Doudna, Jennifer
  • Charpentier, Emmanuelle
subjects:
  • Bacteria
  • Immune Systems
  • Archaea
  • Ribonucleic Acids
  • Online
  • Deoxyribonucleic Acid
  • Immunity
  • Nucleic Acids
  • Miscellaneous Sciences (So)
ispartof: Science, August 17, 2012, Vol.337(6096), pp.816-821
description: Ditching Invading DNABacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas). Jinek et al. (p. 816, published online 28 June; see the Perspective by Brouns) found that for the type II CRISPR/Cas system, the CRISPR RNA (crRNA) as well as the trans-activating crRNA-which is known to be involved in the pre-crRNA processing-were both required to direct the Cas9 endonuclease to cleave the invading target DNA. Furthermore, engineered RNA molecules were able to program the Cas9 endonuclease to cleave specific DNA sequences to generate double-stranded DNA breaks.
language: eng
source:
identifier: ISSN: 0036-8075 ; DOI: 10.1126/science.1225829
fulltext: no_fulltext
issn:
  • 00368075
  • 0036-8075
url: Link


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titleA Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
creatorJinek, Martin ; Chylinski, Krzysztof ; Fonfara, Ines ; Hauer, Michael ; Doudna, Jennifer ; Charpentier, Emmanuelle
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ispartofScience, August 17, 2012, Vol.337(6096), pp.816-821
identifierISSN: 0036-8075 ; DOI: 10.1126/science.1225829
subjectBacteria ; Immune Systems ; Archaea ; Ribonucleic Acids ; Online ; Deoxyribonucleic Acid ; Immunity ; Nucleic Acids ; Miscellaneous Sciences (So)
descriptionDitching Invading DNABacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas). Jinek et al. (p. 816, published online 28 June; see the Perspective by Brouns) found that for the type II CRISPR/Cas system, the CRISPR RNA (crRNA) as well as the trans-activating crRNA-which is known to be involved in the pre-crRNA processing-were both required to direct the Cas9 endonuclease to cleave the invading target DNA. Furthermore, engineered RNA molecules were able to program the Cas9 endonuclease to cleave specific DNA sequences to generate double-stranded DNA breaks.
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descriptionDitching Invading DNABacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas). Jinek et al. (p. 816, published online 28 June; see the Perspective by Brouns) found that for the type II CRISPR/Cas system, the CRISPR RNA (crRNA) as well as the trans-activating crRNA-which is known to be involved in the pre-crRNA processing-were both required to direct the Cas9 endonuclease to cleave the invading target DNA. Furthermore, engineered RNA molecules were able to program the Cas9 endonuclease to cleave specific DNA sequences to generate double-stranded DNA breaks.
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abstractDitching Invading DNABacteria and archaea protect themselves from invasive foreign nucleic acids through an RNA-mediated adaptive immune system called CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas). Jinek et al. (p. 816, published online 28 June; see the Perspective by Brouns) found that for the type II CRISPR/Cas system, the CRISPR RNA (crRNA) as well as the trans-activating crRNA-which is known to be involved in the pre-crRNA processing-were both required to direct the Cas9 endonuclease to cleave the invading target DNA. Furthermore, engineered RNA molecules were able to program the Cas9 endonuclease to cleave specific DNA sequences to generate double-stranded DNA breaks.
doi10.1126/science.1225829
urlhttp://search.proquest.com/docview/1808090978/
eissn10959203
date2012-08-17