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Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'.

Sponge metagenomes are a useful platform to mine cryptic biosynthetic gene clusters responsible for production of natural products involved in the sponge-microbe association. Since numerous sponge-derived bioactive metabolites are biosynthesized by the symbiotic bacteria, this strategy may concurren... Full description

Journal Title: PloS one 2016, Vol.11(10), p.e0164468
Main Author: Nakashima, Yu
Other Authors: Egami, Yoko , Kimura, Miki , Wakimoto, Toshiyuki , Abe, Ikuro
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0164468
Link: http://search.proquest.com/docview/1835416610/?pq-origsite=primo
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title: Metagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'.
format: Article
creator:
  • Nakashima, Yu
  • Egami, Yoko
  • Kimura, Miki
  • Wakimoto, Toshiyuki
  • Abe, Ikuro
subjects:
  • Animals–Enzymology
  • Cyanobacteria–Genetics
  • Gene Transfer, Horizontal–Physiology
  • Metagenome–Genetics
  • Metagenomics–Metabolism
  • Multigene Family–Genetics
  • Oligopeptides–Metabolism
  • Phylogeny–Genetics
  • Polyketide Synthases–Microbiology
  • Porifera–Physiology
  • Symbiosis–Physiology
  • Oligopeptides
  • Kasumigamide
  • Polyketide Synthases
ispartof: PloS one, 2016, Vol.11(10), p.e0164468
description: Sponge metagenomes are a useful platform to mine cryptic biosynthetic gene clusters responsible for production of natural products involved in the sponge-microbe association. Since numerous sponge-derived bioactive metabolites are biosynthesized by the symbiotic bacteria, this strategy may concurrently reveal sponge-symbiont produced compounds. Accordingly, a metagenomic analysis of the Japanese marine sponge Discodermia calyx has resulted in the identification of a hybrid type I polyketide synthase-nonribosomal peptide synthetase gene ( kas ). Bioinformatic analysis of the gene product suggested its involvement in the biosynthesis of kasumigamide, a tetrapeptide originally isolated from freshwater free-living cyanobacterium Microcystis aeruginosa NIES-87. Subsequent investigation of the sponge metabolic profile revealed the presence of kasumigamide in the sponge extract. The kasumigamide producing bacterium was identified as an ‘Entotheonella’ sp. Moreover, an in silico analysis of kas gene homologs uncovered the presence of kas family genes in two additional bacteria from different phyla. The production of kasumigamide by distantly related multiple bacterial strains implicates horizontal gene transfer and raises the potential for a wider distribution across other bacterial groups.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0164468
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleMetagenomic Analysis of the Sponge Discodermia Reveals the Production of the Cyanobacterial Natural Product Kasumigamide by 'Entotheonella'.
creatorNakashima, Yu ; Egami, Yoko ; Kimura, Miki ; Wakimoto, Toshiyuki ; Abe, Ikuro
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ispartofPloS one, 2016, Vol.11(10), p.e0164468
identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0164468
subjectAnimals–Enzymology ; Cyanobacteria–Genetics ; Gene Transfer, Horizontal–Physiology ; Metagenome–Genetics ; Metagenomics–Metabolism ; Multigene Family–Genetics ; Oligopeptides–Metabolism ; Phylogeny–Genetics ; Polyketide Synthases–Microbiology ; Porifera–Physiology ; Symbiosis–Physiology ; Oligopeptides ; Kasumigamide ; Polyketide Synthases
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descriptionSponge metagenomes are a useful platform to mine cryptic biosynthetic gene clusters responsible for production of natural products involved in the sponge-microbe association. Since numerous sponge-derived bioactive metabolites are biosynthesized by the symbiotic bacteria, this strategy may concurrently reveal sponge-symbiont produced compounds. Accordingly, a metagenomic analysis of the Japanese marine sponge Discodermia calyx has resulted in the identification of a hybrid type I polyketide synthase-nonribosomal peptide synthetase gene ( kas ). Bioinformatic analysis of the gene product suggested its involvement in the biosynthesis of kasumigamide, a tetrapeptide originally isolated from freshwater free-living cyanobacterium Microcystis aeruginosa NIES-87. Subsequent investigation of the sponge metabolic profile revealed the presence of kasumigamide in the sponge extract. The kasumigamide producing bacterium was identified as an ‘Entotheonella’ sp. Moreover, an in silico analysis of kas gene homologs uncovered the presence of kas family genes in two additional bacteria from different phyla. The production of kasumigamide by distantly related multiple bacterial strains implicates horizontal gene transfer and raises the potential for a wider distribution across other bacterial groups.
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