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Growth hormone-releasing hormone antagonist inhibits the invasiveness of human endometrial cancer cells by down-regulating twist and N-cadherin expression.

More than 25% of patients diagnosed with endometrial carcinoma have invasive primary cancer accompanied by metastases. Growth hormone-releasing hormone (GHRH) plays an important role in reproduction. Here, we examined the effect of a GHRH antagonist on the motility of endometrial cancer cells and th... Full description

Journal Title: Oncotarget January 17, 2017, Vol.8(3), pp.4410-4421
Main Author: Wu, Hsien-Ming
Other Authors: Huang, Hong-Yuan , Schally, Andrew V , Chao, Angel , Chou, Hung-Hsueh , Leung, Peter C K , Wang, Hsin-Shih
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1949-2553 ; DOI: 10.18632/oncotarget.13877
Link: http://search.proquest.com/docview/1854106559/?pq-origsite=primo
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title: Growth hormone-releasing hormone antagonist inhibits the invasiveness of human endometrial cancer cells by down-regulating twist and N-cadherin expression.
format: Article
creator:
  • Wu, Hsien-Ming
  • Huang, Hong-Yuan
  • Schally, Andrew V
  • Chao, Angel
  • Chou, Hung-Hsueh
  • Leung, Peter C K
  • Wang, Hsin-Shih
subjects:
  • Antigens, CD–Genetics
  • Antineoplastic Agents–Metabolism
  • Cadherins–Genetics
  • Cell Line, Tumor–Metabolism
  • Cell Movement–Drug Effects
  • Cell Proliferation–Drug Effects
  • Dose-Response Relationship, Drug–Drug Therapy
  • Down-Regulation–Genetics
  • Endometrial Neoplasms–Metabolism
  • Female–Drug Effects
  • Gene Expression Regulation, Neoplastic–Antagonists & Inhibitors
  • Gene Knockdown Techniques–Genetics
  • Growth Hormone-Releasing Hormone–Genetics
  • Hela Cells–Metabolism
  • Humans–Pharmacology
  • Nuclear Proteins–Analogs & Derivatives
  • RNA, Small Interfering–Pharmacology
  • Sermorelin–Drug Effects
  • Signal Transduction–Genetics
  • Twist-Related Protein 1–Metabolism
  • Antigens, CD
  • Antineoplastic Agents
  • Cdh2 Protein, Human
  • Cadherins
  • Ghrh(1-29)Nh2, (Phac-Ada)(0)-Tyr(1), Arg(2), Fpa(5,6), Ala(8), Har(9), Tyr(Me)(10), His(11), Orn(12,) Abu(15), His(20), Orn(21), Nle(27), Arg(28), Har(29)-
  • Nuclear Proteins
  • RNA, Small Interfering
  • Twist1 Protein, Human
  • Twist-Related Protein 1
  • Sermorelin
  • Growth Hormone-Releasing Hormone
  • Ghrh Antagonist
  • N-Cadherin
  • Endometrial Cancer
  • Invasion
  • Twist
ispartof: Oncotarget, January 17, 2017, Vol.8(3), pp.4410-4421
description: More than 25% of patients diagnosed with endometrial carcinoma have invasive primary cancer accompanied by metastases. Growth hormone-releasing hormone (GHRH) plays an important role in reproduction. Here, we examined the effect of a GHRH antagonist on the motility of endometrial cancer cells and the mechanisms of action of the antagonist in endometrial cancer. Western blotting and immunohistochemistry (IHC) were used to determine the expression of the GHRH receptor protein. The activity of Twist and N-cadherin was determined by Western blotting. Cell motility was assessed by an invasion and migration assay. GHRH receptor siRNA was applied to knockdown the GHRH receptor in endometrial cancer cells. The GHRH antagonist inhibited cell motility in a dose-dependent manner. The GHRH antagonist inhibited cell motility and suppressed the expression of Twist and N-cadherin, and the suppression was abolished by GHRH receptor siRNA pretreatment. Moreover, the inhibition of Twist and N-cadherin with Twist siRNA and N-cadherin siRNA, respectively, suppressed cell motility. Our study indicates that the GHRH antagonist inhibited the cell motility of endometrial cancer cells through the GHRH receptor via the suppression of Twist and N-cadherin. Our findings represent a new concept in the mechanism of GHRH antagonist-suppressed cell motility in endometrial cancer cells and suggest the possibility of exploring GHRH antagonists as potential therapeutics for the treatment of human endometrial cancer.
language: eng
source:
identifier: E-ISSN: 1949-2553 ; DOI: 10.18632/oncotarget.13877
fulltext: fulltext
issn:
  • 19492553
  • 1949-2553
url: Link


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titleGrowth hormone-releasing hormone antagonist inhibits the invasiveness of human endometrial cancer cells by down-regulating twist and N-cadherin expression.
creatorWu, Hsien-Ming ; Huang, Hong-Yuan ; Schally, Andrew V ; Chao, Angel ; Chou, Hung-Hsueh ; Leung, Peter C K ; Wang, Hsin-Shih
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ispartofOncotarget, January 17, 2017, Vol.8(3), pp.4410-4421
identifierE-ISSN: 1949-2553 ; DOI: 10.18632/oncotarget.13877
subjectAntigens, CD–Genetics ; Antineoplastic Agents–Metabolism ; Cadherins–Genetics ; Cell Line, Tumor–Metabolism ; Cell Movement–Drug Effects ; Cell Proliferation–Drug Effects ; Dose-Response Relationship, Drug–Drug Therapy ; Down-Regulation–Genetics ; Endometrial Neoplasms–Metabolism ; Female–Drug Effects ; Gene Expression Regulation, Neoplastic–Antagonists & Inhibitors ; Gene Knockdown Techniques–Genetics ; Growth Hormone-Releasing Hormone–Genetics ; Hela Cells–Metabolism ; Humans–Pharmacology ; Nuclear Proteins–Analogs & Derivatives ; RNA, Small Interfering–Pharmacology ; Sermorelin–Drug Effects ; Signal Transduction–Genetics ; Twist-Related Protein 1–Metabolism ; Antigens, CD ; Antineoplastic Agents ; Cdh2 Protein, Human ; Cadherins ; Ghrh(1-29)Nh2, (Phac-Ada)(0)-Tyr(1), Arg(2), Fpa(5,6), Ala(8), Har(9), Tyr(Me)(10), His(11), Orn(12,) Abu(15), His(20), Orn(21), Nle(27), Arg(28), Har(29)- ; Nuclear Proteins ; RNA, Small Interfering ; Twist1 Protein, Human ; Twist-Related Protein 1 ; Sermorelin ; Growth Hormone-Releasing Hormone ; Ghrh Antagonist ; N-Cadherin ; Endometrial Cancer ; Invasion ; Twist
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descriptionMore than 25% of patients diagnosed with endometrial carcinoma have invasive primary cancer accompanied by metastases. Growth hormone-releasing hormone (GHRH) plays an important role in reproduction. Here, we examined the effect of a GHRH antagonist on the motility of endometrial cancer cells and the mechanisms of action of the antagonist in endometrial cancer. Western blotting and immunohistochemistry (IHC) were used to determine the expression of the GHRH receptor protein. The activity of Twist and N-cadherin was determined by Western blotting. Cell motility was assessed by an invasion and migration assay. GHRH receptor siRNA was applied to knockdown the GHRH receptor in endometrial cancer cells. The GHRH antagonist inhibited cell motility in a dose-dependent manner. The GHRH antagonist inhibited cell motility and suppressed the expression of Twist and N-cadherin, and the suppression was abolished by GHRH receptor siRNA pretreatment. Moreover, the inhibition of Twist and N-cadherin with Twist siRNA and N-cadherin siRNA, respectively, suppressed cell motility. Our study indicates that the GHRH antagonist inhibited the cell motility of endometrial cancer cells through the GHRH receptor via the suppression of Twist and N-cadherin. Our findings represent a new concept in the mechanism of GHRH antagonist-suppressed cell motility in endometrial cancer cells and suggest the possibility of exploring GHRH antagonists as potential therapeutics for the treatment of human endometrial cancer.
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titleGrowth hormone-releasing hormone antagonist inhibits the invasiveness of human endometrial cancer cells by down-regulating twist and N-cadherin expression.
authorWu, Hsien-Ming ; Huang, Hong-Yuan ; Schally, Andrew V ; Chao, Angel ; Chou, Hung-Hsueh ; Leung, Peter C K ; Wang, Hsin-Shih
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