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A ligation-based loop-mediated isothermal amplification (ligation-LAMP) strategy for highly selective microRNA detection

A novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for miRNA detection, which enables highly selective and sensitive quantitative detection of miR-21 in a dynamic range from 1 fM to 1 nM with the ability to discriminate single-base mismatches.

Journal Title: Chemical Communications: Chem Comm October 2016, Vol.52(86), pp.12721-12724
Main Author: Du, Wenfang
Other Authors: Lv, Mengmei , Li, Junjie , Yu, Ruqin , Jiang, Jianhui
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1359-7345 ; E-ISSN: 1364-548X ; DOI: 10.1039/c6cc06160e
Link: http://search.proquest.com/docview/1855378834/?pq-origsite=primo
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title: A ligation-based loop-mediated isothermal amplification (ligation-LAMP) strategy for highly selective microRNA detection
format: Article
creator:
  • Du, Wenfang
  • Lv, Mengmei
  • Li, Junjie
  • Yu, Ruqin
  • Jiang, Jianhui
subjects:
  • Dynamic Range
  • Amplification
  • Ribonucleic Acids
  • Strategy
  • Miscellaneous Sciences (So)
  • Chemical and Electrochemical Properties (MD)
  • Chemical and Electrochemical Properties (Ep)
  • Chemical and Electrochemical Properties (Ed)
  • Chemical and Electrochemical Properties (EC)
ispartof: Chemical Communications: Chem Comm, October 2016, Vol.52(86), pp.12721-12724
description: A novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for miRNA detection, which enables highly selective and sensitive quantitative detection of miR-21 in a dynamic range from 1 fM to 1 nM with the ability to discriminate single-base mismatches.
language: eng
source:
identifier: ISSN: 1359-7345 ; E-ISSN: 1364-548X ; DOI: 10.1039/c6cc06160e
fulltext: fulltext
issn:
  • 13597345
  • 1359-7345
  • 1364548X
  • 1364-548X
url: Link


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descriptionA novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for miRNA detection, which enables highly selective and sensitive quantitative detection of miR-21 in a dynamic range from 1 fM to 1 nM with the ability to discriminate single-base mismatches.
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abstractA novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for miRNA detection, which enables highly selective and sensitive quantitative detection of miR-21 in a dynamic range from 1 fM to 1 nM with the ability to discriminate single-base mismatches.
doi10.1039/c6cc06160e
urlhttp://search.proquest.com/docview/1855378834/
date2016-10-01