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Periovulatory follicular fluid levels of estradiol trigger inflammatory and DNA damage responses in oviduct epithelial cells

Objective Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher le... Full description

Journal Title: PLoS ONE February 2017, Vol.12(2)
Main Author: Palma-Vera, Sergio
Other Authors: Schoen, Jennifer , Chen, Shuai
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0172192
Link: http://search.proquest.com/docview/1877849705/?pq-origsite=primo
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title: Periovulatory follicular fluid levels of estradiol trigger inflammatory and DNA damage responses in oviduct epithelial cells
format: Article
creator:
  • Palma-Vera, Sergio
  • Schoen, Jennifer
  • Chen, Shuai
subjects:
  • Transformation
  • Epithelial Cells
  • Interfaces
  • Animal Models
  • Cell Culture
  • Steroid Hormones
  • Estradiol
  • Interleukin 8
  • Inflammation
  • Follicular Fluid
  • Gene Expression
  • Differentiation
  • DNA Damage
  • Oviduct
  • Ovulation
  • Epithelium
  • Cell Proliferation
  • Genetic Engineering
  • DNA Metabolism & Structure
ispartof: PLoS ONE, February 2017, Vol.12(2)
description: Objective Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models. Methods A cell line derived from the porcine oviductal epithelium (CCLV-RIE270) was characterized (lineage markers, proliferation characteristics and transformation status). Primary porcine oviduct epithelial cells (POEC) were cultured in air-liquid interface and differentiation was assessed histologically. Both cultures were exposed to E2 (10 ng/ml and 200 ng/ml). Proliferation of CCLV-RIE270 and POEC was determined by real-time impedance monitoring and immunohistochemical detection...
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0172192
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titlePeriovulatory follicular fluid levels of estradiol trigger inflammatory and DNA damage responses in oviduct epithelial cells
creatorPalma-Vera, Sergio ; Schoen, Jennifer ; Chen, Shuai
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0172192
subjectTransformation ; Epithelial Cells ; Interfaces ; Animal Models ; Cell Culture ; Steroid Hormones ; Estradiol ; Interleukin 8 ; Inflammation ; Follicular Fluid ; Gene Expression ; Differentiation ; DNA Damage ; Oviduct ; Ovulation ; Epithelium ; Cell Proliferation ; Genetic Engineering ; DNA Metabolism & Structure
descriptionObjective Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models. Methods A cell line derived from the porcine oviductal epithelium (CCLV-RIE270) was characterized (lineage markers, proliferation characteristics and transformation status). Primary porcine oviduct epithelial cells (POEC) were cultured in air-liquid interface and differentiation was assessed histologically. Both cultures were exposed to E2 (10 ng/ml and 200 ng/ml). Proliferation of CCLV-RIE270 and POEC was determined by real-time impedance monitoring and immunohistochemical detection...
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descriptionObjective Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models. Methods A cell line derived from the porcine oviductal epithelium (CCLV-RIE270) was characterized (lineage markers, proliferation characteristics and transformation status). Primary porcine oviduct epithelial cells (POEC) were cultured in air-liquid interface and differentiation was assessed histologically. Both cultures were exposed to E2 (10 ng/ml and 200 ng/ml). Proliferation of CCLV-RIE270 and POEC was determined by real-time impedance monitoring and immunohistochemical detection...
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abstractObjective Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models. Methods A cell line derived from the porcine oviductal epithelium (CCLV-RIE270) was characterized (lineage markers, proliferation characteristics and transformation status). Primary porcine oviduct epithelial cells (POEC) were cultured in air-liquid interface and differentiation was assessed histologically. Both cultures were exposed to E2 (10 ng/ml and 200 ng/ml). Proliferation of CCLV-RIE270 and POEC was determined by real-time impedance monitoring and immunohistochemical detection...
doi10.1371/journal.pone.0172192
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pagese0172192
date2017-02-01