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Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells

The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interf... Full description

Journal Title: Angewandte Chemie May 15, 2017, Vol.129(21), pp.5923-5927
Main Author: Wang, Danyang
Other Authors: Du, Shubo , Cazenave-Gassiot, Amaury , Ge, Jingyan , Lee, Jun-Seok , Wenk, Markus R , Yao, Shao Q
Format: Electronic Article Electronic Article
Language: ger
Subjects:
ID: ISSN: 00448249 ; E-ISSN: 15213757 ; DOI: 10.1002/ange.201702509
Link: http://search.proquest.com/docview/1896028236/?pq-origsite=primo
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title: Global Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells
format: Article
creator:
  • Wang, Danyang
  • Du, Shubo
  • Cazenave-Gassiot, Amaury
  • Ge, Jingyan
  • Lee, Jun-Seok
  • Wenk, Markus R
  • Yao, Shao Q
subjects:
  • Metabolism
  • Peptide Mapping
  • Lipids
  • Lipids
  • Fatty Acids
  • Proteins
  • Mammalian Cells
  • Choline
  • Alkynes
  • Phospholipids
  • Choline
  • Proteomics
  • Proteins
ispartof: Angewandte Chemie, May 15, 2017, Vol.129(21), pp.5923-5927
description: The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent insitu profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
language: ger
source:
identifier: ISSN: 00448249 ; E-ISSN: 15213757 ; DOI: 10.1002/ange.201702509
fulltext: fulltext
issn:
  • 00448249
  • 0044-8249
  • 15213757
  • 1521-3757
url: Link


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titleGlobal Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells
creatorWang, Danyang ; Du, Shubo ; Cazenave-Gassiot, Amaury ; Ge, Jingyan ; Lee, Jun-Seok ; Wenk, Markus R ; Yao, Shao Q
ispartofAngewandte Chemie, May 15, 2017, Vol.129(21), pp.5923-5927
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subjectMetabolism ; Peptide Mapping ; Lipids ; Lipids ; Fatty Acids ; Proteins ; Mammalian Cells ; Choline ; Alkynes ; Phospholipids ; Choline ; Proteomics ; Proteins
descriptionThe protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent insitu profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
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titleGlobal Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells
descriptionThe protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent insitu profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
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titleGlobal Mapping of Protein-Lipid Interactions by Using Modified Choline-Containing Phospholipids Metabolically Synthesized in Live Cells
authorWang, Danyang ; Du, Shubo ; Cazenave-Gassiot, Amaury ; Ge, Jingyan ; Lee, Jun-Seok ; Wenk, Markus R ; Yao, Shao Q
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abstractThe protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent insitu profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
copWeinheim
pubWiley Subscription Services, Inc.
doi10.1002/ange.201702509
urlhttp://search.proquest.com/docview/1896028236/
date2017-05-15