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Succinct workflows for circulating tumor cells after enrichment: From systematic counting to mutational profiling.

PURPOSEThis study aims to establish a highly adaptable workflow downstream of microfluidic enrichment for facilitating systematic CTC enumeration and genetic discovery. METHODSTo facilitate CTC enumeration, we established a CK/EPCAM-combined immunostaining strategy and an automated CTC analytical pi... Full description

Journal Title: PloS one 2017, Vol.12(5), p.e0177276
Main Author: Wong, Victor Chun-Lam
Other Authors: Ko, Josephine Mun-Yee , Lam, Chi-Tat , Lung, Maria Li
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0177276
Link: http://search.proquest.com/docview/1896891254/?pq-origsite=primo
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title: Succinct workflows for circulating tumor cells after enrichment: From systematic counting to mutational profiling.
format: Article
creator:
  • Wong, Victor Chun-Lam
  • Ko, Josephine Mun-Yee
  • Lam, Chi-Tat
  • Lung, Maria Li
subjects:
  • Female–Pathology
  • Humans–Pathology
  • Male–Pathology
  • Microfluidic Analytical Techniques–Pathology
  • Mutation–Pathology
  • Neoplastic Cells, Circulating–Pathology
ispartof: PloS one, 2017, Vol.12(5), p.e0177276
description: PURPOSEThis study aims to establish a highly adaptable workflow downstream of microfluidic enrichment for facilitating systematic CTC enumeration and genetic discovery. METHODSTo facilitate CTC enumeration, we established a CK/EPCAM-combined immunostaining strategy and an automated CTC analytical pipeline...
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0177276
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titleSuccinct workflows for circulating tumor cells after enrichment: From systematic counting to mutational profiling.
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descriptionPURPOSEThis study aims to establish a highly adaptable workflow downstream of microfluidic enrichment for facilitating systematic CTC enumeration and genetic discovery. METHODSTo facilitate CTC enumeration, we established a CK/EPCAM-combined immunostaining strategy and an automated CTC analytical pipeline...
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abstractPURPOSEThis study aims to establish a highly adaptable workflow downstream of microfluidic enrichment for facilitating systematic CTC enumeration and genetic discovery. METHODSTo facilitate CTC enumeration, we established a CK/EPCAM-combined immunostaining strategy and an automated CTC analytical pipeline...
doi10.1371/journal.pone.0177276
urlhttp://search.proquest.com/docview/1896891254/
date2017-01-01