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A fluorometric aptamer based assay for cytochrome C using fluorescent graphitic carbon nitride nanosheets

A method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. Bulk g-C3N4 was obtained by pyrolysis of melamine at 550 °C. The nanosheets were characterized by sca... Full description

Journal Title: Mikrochimica Acta 2017, Vol.184(7), pp.2157-2163
Main Author: Salehnia, Foad
Other Authors: Hosseini, Morteza , Ganjali, Mohammad
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 00263672 ; E-ISSN: 14365073 ; DOI: 10.1007/s00604-017-2130-6
Link: http://search.proquest.com/docview/1908099146/?pq-origsite=primo
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title: A fluorometric aptamer based assay for cytochrome C using fluorescent graphitic carbon nitride nanosheets
format: Article
creator:
  • Salehnia, Foad
  • Hosseini, Morteza
  • Ganjali, Mohammad
subjects:
  • Excitation
  • Sheets
  • Nanostructure
  • Fluorescence
  • Cytochrome
  • Assaying
  • Emission
  • Carbon Nitride
  • Surface Chemistry
  • Protonation
  • Exfoliation
  • Wavelengths
  • Scanning Electron Microscopy
  • Pyrolysis
  • Melamine
  • Adsorption
  • Quenching
  • X-Ray Diffraction
  • 2d Material
  • G-C3n4nano-Sheets
ispartof: Mikrochimica Acta, 2017, Vol.184(7), pp.2157-2163
description: A method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. Bulk g-C3N4 was obtained by pyrolysis of melamine at 550 °C. The nanosheets were characterized by scanning electron microscopy and X-ray diffraction. The material was then subjected to protonation and exfoliation to obtain blue fluorescent g-C3N4 nano-sheets. The adsorption of aptamers on the surface of the g-C3N4 nanosheets leads to quenching of its fluorescence (measured at excitation/emission wavelengths of 320/450 nm), but fluorescence is restored on addition of cyt c. Response is linear in the 16 to 140 nM cyt c concentration window, and the detection limit is as low as 2.6 nM. The assay is simple, inexpensive, and can be easily performed. It is selective for cyt c over a number of interfering species. The method was successfully applied to the determination of cyt c in spiked human serum...
language: eng
source:
identifier: ISSN: 00263672 ; E-ISSN: 14365073 ; DOI: 10.1007/s00604-017-2130-6
fulltext: fulltext
issn:
  • 00263672
  • 0026-3672
  • 14365073
  • 1436-5073
url: Link


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titleA fluorometric aptamer based assay for cytochrome C using fluorescent graphitic carbon nitride nanosheets
creatorSalehnia, Foad ; Hosseini, Morteza ; Ganjali, Mohammad
ispartofMikrochimica Acta, 2017, Vol.184(7), pp.2157-2163
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subjectExcitation ; Sheets ; Nanostructure ; Fluorescence ; Cytochrome ; Assaying ; Emission ; Carbon Nitride ; Surface Chemistry ; Protonation ; Exfoliation ; Wavelengths ; Scanning Electron Microscopy ; Pyrolysis ; Melamine ; Adsorption ; Quenching ; X-Ray Diffraction ; 2d Material ; G-C3n4nano-Sheets
descriptionA method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. Bulk g-C3N4 was obtained by pyrolysis of melamine at 550 °C. The nanosheets were characterized by scanning electron microscopy and X-ray diffraction. The material was then subjected to protonation and exfoliation to obtain blue fluorescent g-C3N4 nano-sheets. The adsorption of aptamers on the surface of the g-C3N4 nanosheets leads to quenching of its fluorescence (measured at excitation/emission wavelengths of 320/450 nm), but fluorescence is restored on addition of cyt c. Response is linear in the 16 to 140 nM cyt c concentration window, and the detection limit is as low as 2.6 nM. The assay is simple, inexpensive, and can be easily performed. It is selective for cyt c over a number of interfering species. The method was successfully applied to the determination of cyt c in spiked human serum...
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titleA fluorometric aptamer based assay for cytochrome C using fluorescent graphitic carbon nitride nanosheets
descriptionA method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. Bulk g-C3N4 was obtained by pyrolysis of melamine at 550 °C. The nanosheets were characterized by scanning electron microscopy and X-ray diffraction. The material was then subjected to protonation and exfoliation to obtain blue fluorescent g-C3N4 nano-sheets. The adsorption of aptamers on the surface of the g-C3N4 nanosheets leads to quenching of its fluorescence (measured at excitation/emission wavelengths of 320/450 nm), but fluorescence is restored on addition of cyt c. Response is linear in the 16 to 140 nM cyt c concentration window, and the detection limit is as low as 2.6 nM. The assay is simple, inexpensive, and can be easily performed. It is selective for cyt c over a number of interfering species. The method was successfully applied to the determination of cyt c in spiked human serum...
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titleA fluorometric aptamer based assay for cytochrome C using fluorescent graphitic carbon nitride nanosheets
authorSalehnia, Foad ; Hosseini, Morteza ; Ganjali, Mohammad
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abstractA method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. Bulk g-C3N4 was obtained by pyrolysis of melamine at 550 °C. The nanosheets were characterized by scanning electron microscopy and X-ray diffraction. The material was then subjected to protonation and exfoliation to obtain blue fluorescent g-C3N4 nano-sheets. The adsorption of aptamers on the surface of the g-C3N4 nanosheets leads to quenching of its fluorescence (measured at excitation/emission wavelengths of 320/450 nm), but fluorescence is restored on addition of cyt c. Response is linear in the 16 to 140 nM cyt c concentration window, and the detection limit is as low as 2.6 nM. The assay is simple, inexpensive, and can be easily performed. It is selective for cyt c over a number of interfering species. The method was successfully applied to the determination of cyt c in spiked human serum...
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doi10.1007/s00604-017-2130-6
urlhttp://search.proquest.com/docview/1908099146/
date2017-07-01