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Period2 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.

We previously created two PER2::LUCIFERASE (PER2::LUC) circadian reporter knockin mice that differ only in the Per2 3'-UTR region: Per2::Luc, which retains the endogenous Per2 3'-UTR and Per2::LucSV, where the endogenous Per2 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vi... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America October 17, 2017, Vol.114(42), pp.E8855-E8864
Main Author: Yoo, Seung-Hee
Other Authors: Kojima, Shihoko , Shimomura, Kazuhiro , Koike, Nobuya , Buhr, Ethan D , Furukawa, Tadashi , Ko, Caroline H , Gloston, Gabrielle , Ayoub, Christopher , Nohara, Kazunari , Reyes, Bryan A , Tsuchiya, Yoshiki , Yoo, Ook-Joon , Yagita, Kazuhiro , Lee, Choogon , Chen, Zheng , Yamazaki, Shin , Green, Carla B , Takahashi, Joseph S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1706611114
Link: http://search.proquest.com/docview/1947098567/?pq-origsite=primo
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title: Period2 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.
format: Article
creator:
  • Yoo, Seung-Hee
  • Kojima, Shihoko
  • Shimomura, Kazuhiro
  • Koike, Nobuya
  • Buhr, Ethan D
  • Furukawa, Tadashi
  • Ko, Caroline H
  • Gloston, Gabrielle
  • Ayoub, Christopher
  • Nohara, Kazunari
  • Reyes, Bryan A
  • Tsuchiya, Yoshiki
  • Yoo, Ook-Joon
  • Yagita, Kazuhiro
  • Lee, Choogon
  • Chen, Zheng
  • Yamazaki, Shin
  • Green, Carla B
  • Takahashi, Joseph S
subjects:
  • 3' Untranslated Regions–Genetics
  • Animals–Physiology
  • Circadian Clocks–Genetics
  • Circadian Rhythm–Genetics
  • Gene Expression Regulation–Genetics
  • Gene Knock-In Techniques–Metabolism
  • Luciferases–Metabolism
  • Mice, Inbred C57bl–Metabolism
  • Mice, Transgenic–Metabolism
  • Micrornas–Metabolism
  • Period Circadian Proteins–Metabolism
  • Protein Biosynthesis–Metabolism
  • Temperature–Metabolism
  • 3' Untranslated Regions
  • Micrornas
  • Mirn24 Microrna, Mouse
  • Per2 Protein, Mouse
  • Period Circadian Proteins
  • Luciferases
  • 3′-Utr Regulation
  • Per2 Gene
  • Circadian
  • Mir-24
  • Microrna
ispartof: Proceedings of the National Academy of Sciences of the United States of America, October 17, 2017, Vol.114(42), pp.E8855-E8864
description: We previously created two PER2::LUCIFERASE (PER2::LUC) circadian reporter knockin mice that differ only in the Per2 3'-UTR region: Per2::Luc, which retains the endogenous Per2 3'-UTR and Per2::LucSV, where the endogenous Per2 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vivo functions of Per2 3'-UTR, we analyzed circadian rhythms of Per2::LucSV mice. Interestingly, Per2::LucSV mice displayed more than threefold stronger amplitude in bioluminescence rhythms than Per2::Luc mice, and also exhibited lengthened free-running periods (∼24.0 h), greater phase delays following light pulse, and enhanced temperature compensation relative to Per2::Luc Analysis of the Per2 3'-UTR sequence revealed that miR-24, and to a lesser degree miR-30, suppressed PER2 protein translation, and the reversal of this inhibition in Per2::LucSV augmented PER2::LUC protein level and oscillatory amplitude. Interestingly, Bmal1 mRNA and protein oscillatory amplitude as well as CRY1 protein oscillation were increased in Per2::LucSV mice, suggesting rhythmic overexpression of PER2 enhances expression of Per2 and other core clock genes. Together, these studies provide important mechanistic insights into the regulatory roles of Per2 3'-UTR, miR-24, and PER2 in Per2 expression and core clock function.
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1706611114
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titlePeriod2 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.
creatorYoo, Seung-Hee ; Kojima, Shihoko ; Shimomura, Kazuhiro ; Koike, Nobuya ; Buhr, Ethan D ; Furukawa, Tadashi ; Ko, Caroline H ; Gloston, Gabrielle ; Ayoub, Christopher ; Nohara, Kazunari ; Reyes, Bryan A ; Tsuchiya, Yoshiki ; Yoo, Ook-Joon ; Yagita, Kazuhiro ; Lee, Choogon ; Chen, Zheng ; Yamazaki, Shin ; Green, Carla B ; Takahashi, Joseph S
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ispartofProceedings of the National Academy of Sciences of the United States of America, October 17, 2017, Vol.114(42), pp.E8855-E8864
identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1706611114
subject3' Untranslated Regions–Genetics ; Animals–Physiology ; Circadian Clocks–Genetics ; Circadian Rhythm–Genetics ; Gene Expression Regulation–Genetics ; Gene Knock-In Techniques–Metabolism ; Luciferases–Metabolism ; Mice, Inbred C57bl–Metabolism ; Mice, Transgenic–Metabolism ; Micrornas–Metabolism ; Period Circadian Proteins–Metabolism ; Protein Biosynthesis–Metabolism ; Temperature–Metabolism ; 3' Untranslated Regions ; Micrornas ; Mirn24 Microrna, Mouse ; Per2 Protein, Mouse ; Period Circadian Proteins ; Luciferases ; 3′-Utr Regulation ; Per2 Gene ; Circadian ; Mir-24 ; Microrna
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descriptionWe previously created two PER2::LUCIFERASE (PER2::LUC) circadian reporter knockin mice that differ only in the Per2 3'-UTR region: Per2::Luc, which retains the endogenous Per2 3'-UTR and Per2::LucSV, where the endogenous Per2 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vivo functions of Per2 3'-UTR, we analyzed circadian rhythms of Per2::LucSV mice. Interestingly, Per2::LucSV mice displayed more than threefold stronger amplitude in bioluminescence rhythms than Per2::Luc mice, and also exhibited lengthened free-running periods (∼24.0 h), greater phase delays following light pulse, and enhanced temperature compensation relative to Per2::Luc Analysis of the Per2 3'-UTR sequence revealed that miR-24, and to a lesser degree miR-30, suppressed PER2 protein translation, and the reversal of this inhibition in Per2::LucSV augmented PER2::LUC protein level and oscillatory amplitude. Interestingly, Bmal1 mRNA and protein oscillatory amplitude as well as CRY1 protein oscillation were increased in Per2::LucSV mice, suggesting rhythmic overexpression of PER2 enhances expression of Per2 and other core clock genes. Together, these studies provide important mechanistic insights into the regulatory roles of Per2 3'-UTR, miR-24, and PER2 in Per2 expression and core clock function.
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17Green, Carla B
18Takahashi, Joseph S
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titlePeriod2 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.
authorYoo, Seung-Hee ; Kojima, Shihoko ; Shimomura, Kazuhiro ; Koike, Nobuya ; Buhr, Ethan D ; Furukawa, Tadashi ; Ko, Caroline H ; Gloston, Gabrielle ; Ayoub, Christopher ; Nohara, Kazunari ; Reyes, Bryan A ; Tsuchiya, Yoshiki ; Yoo, Ook-Joon ; Yagita, Kazuhiro ; Lee, Choogon ; Chen, Zheng ; Yamazaki, Shin...
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6Luciferases–Metabolism
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atitlePeriod2 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.
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