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Raman Deuterium Isotope Probing Reveals Microbial Metabolism at the Single-Cell Level.

We illustrate that single-cell Raman microspectroscopy, coupled with deuterium isotope probing (Raman-DIP), provides a culture-independent and nondestructive approach to probe metabolic pathways of carbon substrates at the single-cell level. We found a distinguishable C-D vibration band at 2070-2300... Full description

Journal Title: Analytical chemistry December 19, 2017, Vol.89(24), pp.13305-13312
Main Author: Xu, Jiabao
Other Authors: Zhu, Di , Ibrahim, Aliyu D , Allen, Christopher C R , Gibson, Christopher M , Fowler, Patrick W , Song, Yizhi , Huang, Wei E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.7b03461
Link: http://search.proquest.com/docview/1964699538/?pq-origsite=primo
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title: Raman Deuterium Isotope Probing Reveals Microbial Metabolism at the Single-Cell Level.
format: Article
creator:
  • Xu, Jiabao
  • Zhu, Di
  • Ibrahim, Aliyu D
  • Allen, Christopher C R
  • Gibson, Christopher M
  • Fowler, Patrick W
  • Song, Yizhi
  • Huang, Wei E
subjects:
  • Deuterium–Chemistry
  • Escherichia Coli–Metabolism
  • Glucose–Metabolism
  • Naphthalenes–Metabolism
  • Pseudomonas–Metabolism
  • Single-Cell Analysis–Metabolism
  • Spectrum Analysis, Raman–Metabolism
  • Naphthalenes
  • Naphthalene
  • Deuterium
  • Glucose
ispartof: Analytical chemistry, December 19, 2017, Vol.89(24), pp.13305-13312
description: We illustrate that single-cell Raman microspectroscopy, coupled with deuterium isotope probing (Raman-DIP), provides a culture-independent and nondestructive approach to probe metabolic pathways of carbon substrates at the single-cell level. We found a distinguishable C-D vibration band at 2070-2300 cm in single-cell Raman spectra (SCRS) when Escherichia coli used deuterated glucose and Pseudomonas sp. used deuterated naphthalene as sole carbon sources. The intensity of the C-D band is proportional to the extent of deuteration in the carbon source, and as little as 5% deuteration can be distinguished by analysis of SCRS. It suggests that Raman-DIP could be used to semiquantitatively and sensitively indicate the metabolism of deuterated carbon source in microbes. A lower lipid conversion rate of deuterated naphthalene compared to that of deuterated glucose was observed, presumably owing to different anabolic pathways and membrane alteration. Apart from the C-D band shift from C-H, SCRS also...
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.7b03461
fulltext: fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleRaman Deuterium Isotope Probing Reveals Microbial Metabolism at the Single-Cell Level.
creatorXu, Jiabao ; Zhu, Di ; Ibrahim, Aliyu D ; Allen, Christopher C R ; Gibson, Christopher M ; Fowler, Patrick W ; Song, Yizhi ; Huang, Wei E
contributorXu, Jiabao (correspondence author) ; Xu, Jiabao (record owner)
ispartofAnalytical chemistry, December 19, 2017, Vol.89(24), pp.13305-13312
identifierE-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.7b03461
subjectDeuterium–Chemistry ; Escherichia Coli–Metabolism ; Glucose–Metabolism ; Naphthalenes–Metabolism ; Pseudomonas–Metabolism ; Single-Cell Analysis–Metabolism ; Spectrum Analysis, Raman–Metabolism ; Naphthalenes ; Naphthalene ; Deuterium ; Glucose
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descriptionWe illustrate that single-cell Raman microspectroscopy, coupled with deuterium isotope probing (Raman-DIP), provides a culture-independent and nondestructive approach to probe metabolic pathways of carbon substrates at the single-cell level. We found a distinguishable C-D vibration band at 2070-2300 cm in single-cell Raman spectra (SCRS) when Escherichia coli used deuterated glucose and Pseudomonas sp. used deuterated naphthalene as sole carbon sources. The intensity of the C-D band is proportional to the extent of deuteration in the carbon source, and as little as 5% deuteration can be distinguished by analysis of SCRS. It suggests that Raman-DIP could be used to semiquantitatively and sensitively indicate the metabolism of deuterated carbon source in microbes. A lower lipid conversion rate of deuterated naphthalene compared to that of deuterated glucose was observed, presumably owing to different anabolic pathways and membrane alteration. Apart from the C-D band shift from C-H, SCRS also...
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