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Alteration of fecal bacterial communities in weanling pigs fed diets supplemented with chicory, mannan oligosaccharides, or chitosan

Our previous study demonstrated that dietary prebiotics did not affect growth performance and serum IgA of weanling pigs, except that feeding mannan oligosaccharides (MOS) decreased G:F compared to the other treatments. To determine changes in fecal bacterial communities, 64 weanling pigs were allot... Full description

Journal Title: Journal of Animal Science Apr 2016, Vol.94, p.138
Main Author: Li, Y
Other Authors: Fernando, S , Miller, P , Burkey, T
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 00218812 ; E-ISSN: 15253163 ; DOI: 10.2527/msasas2016-294
Link: http://search.proquest.com/docview/2036980029/?pq-origsite=primo
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title: Alteration of fecal bacterial communities in weanling pigs fed diets supplemented with chicory, mannan oligosaccharides, or chitosan
format: Article
creator:
  • Li, Y
  • Fernando, S
  • Miller, P
  • Burkey, T
subjects:
  • Lactobacillaceae
  • Cichorium
  • Hogs
  • Feces
  • Bacteria
  • Prebiotics
  • Weaning
  • Community Composition
  • Rrna 16s
  • Diet
  • Bacteria
  • Chitosan
  • Mannan
  • Community Composition
  • Oligosaccharides
  • Chicory
  • Communities
  • Diet
  • Animal Models
  • Oligosaccharides
  • Chitosan
  • Bacteria
  • Diet
  • Immunoglobulin A
ispartof: Journal of Animal Science, Apr 2016, Vol.94, p.138
description: Our previous study demonstrated that dietary prebiotics did not affect growth performance and serum IgA of weanling pigs, except that feeding mannan oligosaccharides (MOS) decreased G:F compared to the other treatments. To determine changes in fecal bacterial communities, 64 weanling pigs were allotted to 16 pens with 4 dietary treatments. The control diet was a complex nursery diet and the additional 3 diets contained 0.1% chicory. 0.1% MOS, and 0.02% chitosan, respectively. Fecal samples from 2 pigs/pen were collected on d 0, 14, and 28 postweaning to evaluate bacterial community composition using 16S rRNA tag sequencing. Database independent operational taxonomic unit (OTU) approach was used for bacterial community analysis. The a-diversity estimate (Cliao 1) was different (P 0.10) by treatment. Additionally, 3-diver-sity changed over time (P 0.10) from MOS. Linear discriminate analysis (LDA) by LEfSe was used for pair-wise comparisons of OTU abundances in the bacterial communities (|LDA score) > 2 was considered significant). On d 14, control and MOS had 26 and 12 OTUs, respectively more abundant in the 3 comparisons with other treatments, in which the majority of these OTUs belonged to family Lactobacillaceae. Additionally, 25 and 17 OTUs, respectively that predominantly belonged to family Rumi-nococcaceae and Christensenellaceae were associated with chicory and chitosan. Using multivariate association with linear models (MaAsLin; P 0.012). Mocosae spp. was associated (co-eff= 0.092) with IgA. One OTU belonging to family Christensenellaceae was negatively correlated (coeff. = -0.01) to BW. Family level classification of OTUs was analyzed using ANOVA1 ofMATLAB. On d 14, Lactobacillaceae decreased (P < 0.05) in chicory (18.4%) compared to control (46.5%) and MOS (41.7%); whereas, Rutninoccocaceae increased (P< 0.05) in chicory (15.4%) and chitosan (15.0%) compared to control (10.6%) and MOS (10.3%). Chicory had greater (P< 0.05) Christensenellaceae (5.6%) compared to the other 3 treatments (< 1.8%). On d 28, Streptococcaceae increased (P< 0.05) in control (27.2%) compared to chicory (8.7%) and chitosan (9.5%). In conclusion, pigs fed MOS shared a similar bacterial community with control; however, bacterial communities in chicory and chitosan were similar.
language: eng
source:
identifier: ISSN: 00218812 ; E-ISSN: 15253163 ; DOI: 10.2527/msasas2016-294
fulltext: fulltext
issn:
  • 00218812
  • 0021-8812
  • 15253163
  • 1525-3163
url: Link


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titleAlteration of fecal bacterial communities in weanling pigs fed diets supplemented with chicory, mannan oligosaccharides, or chitosan
creatorLi, Y ; Fernando, S ; Miller, P ; Burkey, T
ispartofJournal of Animal Science, Apr 2016, Vol.94, p.138
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subjectLactobacillaceae ; Cichorium ; Hogs ; Feces ; Bacteria ; Prebiotics ; Weaning ; Community Composition ; Rrna 16s ; Diet ; Bacteria ; Chitosan ; Mannan ; Community Composition ; Oligosaccharides ; Chicory ; Communities ; Diet ; Animal Models ; Oligosaccharides ; Chitosan ; Bacteria ; Diet ; Immunoglobulin A
descriptionOur previous study demonstrated that dietary prebiotics did not affect growth performance and serum IgA of weanling pigs, except that feeding mannan oligosaccharides (MOS) decreased G:F compared to the other treatments. To determine changes in fecal bacterial communities, 64 weanling pigs were allotted to 16 pens with 4 dietary treatments. The control diet was a complex nursery diet and the additional 3 diets contained 0.1% chicory. 0.1% MOS, and 0.02% chitosan, respectively. Fecal samples from 2 pigs/pen were collected on d 0, 14, and 28 postweaning to evaluate bacterial community composition using 16S rRNA tag sequencing. Database independent operational taxonomic unit (OTU) approach was used for bacterial community analysis. The a-diversity estimate (Cliao 1) was different (P 0.10) by treatment. Additionally, 3-diver-sity changed over time (P 0.10) from MOS. Linear discriminate analysis (LDA) by LEfSe was used for pair-wise comparisons of OTU abundances in the bacterial communities (|LDA score) > 2 was considered significant). On d 14, control and MOS had 26 and 12 OTUs, respectively more abundant in the 3 comparisons with other treatments, in which the majority of these OTUs belonged to family Lactobacillaceae. Additionally, 25 and 17 OTUs, respectively that predominantly belonged to family Rumi-nococcaceae and Christensenellaceae were associated with chicory and chitosan. Using multivariate association with linear models (MaAsLin; P 0.012). Mocosae spp. was associated (co-eff= 0.092) with IgA. One OTU belonging to family Christensenellaceae was negatively correlated (coeff. = -0.01) to BW. Family level classification of OTUs was analyzed using ANOVA1 ofMATLAB. On d 14, Lactobacillaceae decreased (P < 0.05) in chicory (18.4%) compared to control (46.5%) and MOS (41.7%); whereas, Rutninoccocaceae increased (P< 0.05) in chicory (15.4%) and chitosan (15.0%) compared to control (10.6%) and MOS (10.3%). Chicory had greater (P< 0.05) Christensenellaceae (5.6%) compared to the other 3 treatments (< 1.8%). On d 28, Streptococcaceae increased (P< 0.05) in control (27.2%) compared to chicory (8.7%) and chitosan (9.5%). In conclusion, pigs fed MOS shared a similar bacterial community with control; however, bacterial communities in chicory and chitosan were similar.
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titleAlteration of fecal bacterial communities in weanling pigs fed diets supplemented with chicory, mannan oligosaccharides, or chitosan
descriptionOur previous study demonstrated that dietary prebiotics did not affect growth performance and serum IgA of weanling pigs, except that feeding mannan oligosaccharides (MOS) decreased G:F compared to the other treatments. To determine changes in fecal bacterial communities, 64 weanling pigs were allotted to 16 pens with 4 dietary treatments. The control diet was a complex nursery diet and the additional 3 diets contained 0.1% chicory. 0.1% MOS, and 0.02% chitosan, respectively. Fecal samples from 2 pigs/pen were collected on d 0, 14, and 28 postweaning to evaluate bacterial community composition using 16S rRNA tag sequencing. Database independent operational taxonomic unit (OTU) approach was used for bacterial community analysis. The a-diversity estimate (Cliao 1) was different (P 0.10) by treatment. Additionally, 3-diver-sity changed over time (P 0.10) from MOS. Linear discriminate analysis (LDA) by LEfSe was used for pair-wise comparisons of OTU abundances in the bacterial communities (|LDA score) > 2 was considered significant). On d 14, control and MOS had 26 and 12 OTUs, respectively more abundant in the 3 comparisons with other treatments, in which the majority of these OTUs belonged to family Lactobacillaceae. Additionally, 25 and 17 OTUs, respectively that predominantly belonged to family Rumi-nococcaceae and Christensenellaceae were associated with chicory and chitosan. Using multivariate association with linear models (MaAsLin; P 0.012). Mocosae spp. was associated (co-eff= 0.092) with IgA. One OTU belonging to family Christensenellaceae was negatively correlated (coeff. = -0.01) to BW. Family level classification of OTUs was analyzed using ANOVA1 ofMATLAB. On d 14, Lactobacillaceae decreased (P < 0.05) in chicory (18.4%) compared to control (46.5%) and MOS (41.7%); whereas, Rutninoccocaceae increased (P< 0.05) in chicory (15.4%) and chitosan (15.0%) compared to control (10.6%) and MOS (10.3%). Chicory had greater (P< 0.05) Christensenellaceae (5.6%) compared to the other 3 treatments (< 1.8%). On d 28, Streptococcaceae increased (P< 0.05) in control (27.2%) compared to chicory (8.7%) and chitosan (9.5%). In conclusion, pigs fed MOS shared a similar bacterial community with control; however, bacterial communities in chicory and chitosan were similar.
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titleAlteration of fecal bacterial communities in weanling pigs fed diets supplemented with chicory, mannan oligosaccharides, or chitosan
authorLi, Y ; Fernando, S ; Miller, P ; Burkey, T
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7Community Composition
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9Diet
10Chitosan
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abstractOur previous study demonstrated that dietary prebiotics did not affect growth performance and serum IgA of weanling pigs, except that feeding mannan oligosaccharides (MOS) decreased G:F compared to the other treatments. To determine changes in fecal bacterial communities, 64 weanling pigs were allotted to 16 pens with 4 dietary treatments. The control diet was a complex nursery diet and the additional 3 diets contained 0.1% chicory. 0.1% MOS, and 0.02% chitosan, respectively. Fecal samples from 2 pigs/pen were collected on d 0, 14, and 28 postweaning to evaluate bacterial community composition using 16S rRNA tag sequencing. Database independent operational taxonomic unit (OTU) approach was used for bacterial community analysis. The a-diversity estimate (Cliao 1) was different (P 0.10) by treatment. Additionally, 3-diver-sity changed over time (P 0.10) from MOS. Linear discriminate analysis (LDA) by LEfSe was used for pair-wise comparisons of OTU abundances in the bacterial communities (|LDA score) > 2 was considered significant). On d 14, control and MOS had 26 and 12 OTUs, respectively more abundant in the 3 comparisons with other treatments, in which the majority of these OTUs belonged to family Lactobacillaceae. Additionally, 25 and 17 OTUs, respectively that predominantly belonged to family Rumi-nococcaceae and Christensenellaceae were associated with chicory and chitosan. Using multivariate association with linear models (MaAsLin; P 0.012). Mocosae spp. was associated (co-eff= 0.092) with IgA. One OTU belonging to family Christensenellaceae was negatively correlated (coeff. = -0.01) to BW. Family level classification of OTUs was analyzed using ANOVA1 ofMATLAB. On d 14, Lactobacillaceae decreased (P < 0.05) in chicory (18.4%) compared to control (46.5%) and MOS (41.7%); whereas, Rutninoccocaceae increased (P< 0.05) in chicory (15.4%) and chitosan (15.0%) compared to control (10.6%) and MOS (10.3%). Chicory had greater (P< 0.05) Christensenellaceae (5.6%) compared to the other 3 treatments (< 1.8%). On d 28, Streptococcaceae increased (P< 0.05) in control (27.2%) compared to chicory (8.7%) and chitosan (9.5%). In conclusion, pigs fed MOS shared a similar bacterial community with control; however, bacterial communities in chicory and chitosan were similar.
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doi10.2527/msasas2016-294
urlhttp://search.proquest.com/docview/2036980029/
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date2016-04-01