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Potential involvement of the 18 kDa translocator protein and reactive oxygen species in apoptosis of THP-1 macrophages induced by sonodynamic therapy.

Sonodynamic therapy (SDT) with exogenous protoporphyrin IX (PpIX) or endogenous PpIX derived from 5-aminolevulinic acid (ALA) has been carried out to produce apoptotic effects on macrophages, indicating a potential treatment methodology for atherosclerosis. Our previous studies have found that mitoc... Full description

Journal Title: PloS one 2018, Vol.13(5), p.e0196541
Main Author: Sun, Xin
Other Authors: Guo, Shuyuan , Wang, Wei , Cao, Zhengyu , Dan, Juhua , Cheng, Jiali , Cao, Wei , Tian, Fang , Cao, Wenwu , Tian, Ye
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0196541
Link: http://search.proquest.com/docview/2038271579/?pq-origsite=primo
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title: Potential involvement of the 18 kDa translocator protein and reactive oxygen species in apoptosis of THP-1 macrophages induced by sonodynamic therapy.
format: Article
creator:
  • Sun, Xin
  • Guo, Shuyuan
  • Wang, Wei
  • Cao, Zhengyu
  • Dan, Juhua
  • Cheng, Jiali
  • Cao, Wei
  • Tian, Fang
  • Cao, Wenwu
  • Tian, Ye
subjects:
  • Aminolevulinic Acid–Pharmacology
  • Apoptosis–Drug Effects
  • Cell Survival–Physiology
  • Cytochromes C–Drug Effects
  • Humans–Metabolism
  • Macrophages–Metabolism
  • Membrane Potential, Mitochondrial–Physiology
  • Mitochondria–Drug Effects
  • Protoporphyrins–Metabolism
  • Reactive Oxygen Species–Metabolism
  • Receptors, Gaba–Metabolism
  • Thp-1 Cells–Physiology
  • Ultrasonic Therapy–Physiology
  • Ultrasonic Therapy–Methods
  • Protoporphyrins
  • Reactive Oxygen Species
  • Receptors, Gaba
  • Tspo Protein, Human
  • Aminolevulinic Acid
  • Cytochromes C
  • Protoporphyrin IX
ispartof: PloS one, 2018, Vol.13(5), p.e0196541
description: Sonodynamic therapy (SDT) with exogenous protoporphyrin IX (PpIX) or endogenous PpIX derived from 5-aminolevulinic acid (ALA) has been carried out to produce apoptotic effects on macrophages, indicating a potential treatment methodology for atherosclerosis. Our previous studies have found that mitochondria damage by reactive oxygen species (ROS) plays a major role in the SDT-induced apoptosis. This study aimed at investigating the potential involvement of the mitochondrial 18 kDa translocator protein (TSPO) and ROS in the pro-apoptotic effects of SDT on THP-1 macrophages. THP-1 macrophages were divided into control and SDT groups, and went through pretreatment of the specific TSPO ligand PK11195 and ROS scavengers N-Acetyl Cysteine (NAC), then compared with groups without pretreatment. Application of PK11195 reduced intracellular accumulation of endogenous PpIX. PK11195 and NAC reduced the generation of intracellular ROS and oxidation of cardiolipin induced by SDT, respectively. PK11195 and NAC also reduced SDT-induced mitochondrial membrane potential ([DELTA][PSI].sub.m) loss, the translocation of cytochrome c and cell apoptosis. PpIX accumulation, ROS generation and cell apoptosis were also attenuated by siTSPO. Our findings indicate the pivotal role of TSPO and ROS in SDT-induced cardiolipin oxidation, [DELTA][PSI].sub.m collapse, cytochrome c translocation and apoptosis in THP-1 macrophages.
language: eng
source:
identifier: E-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0196541
fulltext: fulltext
issn:
  • 19326203
  • 1932-6203
url: Link


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titlePotential involvement of the 18 kDa translocator protein and reactive oxygen species in apoptosis of THP-1 macrophages induced by sonodynamic therapy.
creatorSun, Xin ; Guo, Shuyuan ; Wang, Wei ; Cao, Zhengyu ; Dan, Juhua ; Cheng, Jiali ; Cao, Wei ; Tian, Fang ; Cao, Wenwu ; Tian, Ye
contributorSun, Xin (correspondence author) ; Sun, Xin (record owner)
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identifierE-ISSN: 1932-6203 ; DOI: 10.1371/journal.pone.0196541
subjectAminolevulinic Acid–Pharmacology ; Apoptosis–Drug Effects ; Cell Survival–Physiology ; Cytochromes C–Drug Effects ; Humans–Metabolism ; Macrophages–Metabolism ; Membrane Potential, Mitochondrial–Physiology ; Mitochondria–Drug Effects ; Protoporphyrins–Metabolism ; Reactive Oxygen Species–Metabolism ; Receptors, Gaba–Metabolism ; Thp-1 Cells–Physiology ; Ultrasonic Therapy–Physiology ; Ultrasonic Therapy–Methods ; Protoporphyrins ; Reactive Oxygen Species ; Receptors, Gaba ; Tspo Protein, Human ; Aminolevulinic Acid ; Cytochromes C ; Protoporphyrin IX
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descriptionSonodynamic therapy (SDT) with exogenous protoporphyrin IX (PpIX) or endogenous PpIX derived from 5-aminolevulinic acid (ALA) has been carried out to produce apoptotic effects on macrophages, indicating a potential treatment methodology for atherosclerosis. Our previous studies have found that mitochondria damage by reactive oxygen species (ROS) plays a major role in the SDT-induced apoptosis. This study aimed at investigating the potential involvement of the mitochondrial 18 kDa translocator protein (TSPO) and ROS in the pro-apoptotic effects of SDT on THP-1 macrophages. THP-1 macrophages were divided into control and SDT groups, and went through pretreatment of the specific TSPO ligand PK11195 and ROS scavengers N-Acetyl Cysteine (NAC), then compared with groups without pretreatment. Application of PK11195 reduced intracellular accumulation of endogenous PpIX. PK11195 and NAC reduced the generation of intracellular ROS and oxidation of cardiolipin induced by SDT, respectively. PK11195 and NAC also reduced SDT-induced mitochondrial membrane potential ([DELTA][PSI].sub.m) loss, the translocation of cytochrome c and cell apoptosis. PpIX accumulation, ROS generation and cell apoptosis were also attenuated by siTSPO. Our findings indicate the pivotal role of TSPO and ROS in SDT-induced cardiolipin oxidation, [DELTA][PSI].sub.m collapse, cytochrome c translocation and apoptosis in THP-1 macrophages.
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titlePotential involvement of the 18 kDa translocator protein and reactive oxygen species in apoptosis of THP-1 macrophages induced by sonodynamic therapy.
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titlePotential involvement of the 18 kDa translocator protein and reactive oxygen species in apoptosis of THP-1 macrophages induced by sonodynamic therapy.
authorSun, Xin ; Guo, Shuyuan ; Wang, Wei ; Cao, Zhengyu ; Dan, Juhua ; Cheng, Jiali ; Cao, Wei ; Tian, Fang ; Cao, Wenwu ; Tian, Ye
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