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Measurement of Cell-Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture.

Cell adhesion is essential for a cell to maintain its functions, and biomaterials acting as the extracellular matrix (ECM) play a vital role. However, conventional methods for evaluating the functions of biomaterials become insufficient and sometimes incorrect when we give a deeper insight into sing... Full description

Journal Title: Analytical chemistry August 7, 2018, Vol.90(15), pp.9637-9643
Main Author: Mao, Sifeng
Other Authors: Zhang, Qiang , Li, Haifang , Huang, Qiushi , Khan, Mashooq , Uchiyama, Katsumi , Lin, Jin-Ming
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/acs.analchem.8b02653
Link: http://search.proquest.com/docview/2071573712/?pq-origsite=primo
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title: Measurement of Cell-Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture.
format: Article
creator:
  • Mao, Sifeng
  • Zhang, Qiang
  • Li, Haifang
  • Huang, Qiushi
  • Khan, Mashooq
  • Uchiyama, Katsumi
  • Lin, Jin-Ming
subjects:
  • Cell Adhesion–Chemistry
  • Cell Culture Techniques–Chemistry
  • Cell Line–Chemistry
  • Coated Materials, Biocompatible–Chemistry
  • Equipment Design–Chemistry
  • Extracellular Matrix–Chemistry
  • Fibronectins–Chemistry
  • Glass–Instrumentation
  • Humans–Methods
  • Lab-On-A-Chip Devices–Methods
  • Polylysine–Methods
  • Propylamines–Methods
  • Silanes–Methods
  • Single-Cell Analysis–Methods
  • Surface Properties–Methods
  • Coated Materials, Biocompatible
  • Fibronectins
  • Propylamines
  • Silanes
  • Octadecyltrichlorosilane
  • Polylysine
  • Amino-Propyl-Triethoxysilane
ispartof: Analytical chemistry, August 7, 2018, Vol.90(15), pp.9637-9643
description: Cell adhesion is essential for a cell to maintain its functions, and biomaterials acting as the extracellular matrix (ECM) play a vital role. However, conventional methods for evaluating the functions of biomaterials become insufficient and sometimes incorrect when we give a deeper insight into single-cell research. In this work, we reported a novel methodology for the measurement of cell-matrix adhesion at single-cell resolution that could precisely evaluate the functions of biomaterials for adherent cell culture. A microfludic device, a live single-cell extractor (LSCE), was used for cell extraction. We applied this method to evaluate various modified biomaterials. The results indicated that poly(l-polylysine) (PLL)-coated glass and fibronection (FN)-coated glass slides showed the best biocompatibility for adherent cell culture following by the (3-aminopropyl)triethoxysilane (APTES)-coated glass, while piranha solution treated glass slide and octadecyltrichlorosilane (OTS)-coated glass showed weak biocompatibilities. Furthermore, APTES, PLL, and FN modifications enhanced the cell heterogeneity, while the OTS modification weakened the cell heterogeneity compare to the initial piranha solution treated glass. The method not only clarified the cell-matrix adhesion strength at single-cell resolution but also revealed the influences of biomaterials on cell-matrix adhesion and heterogeneity of cell-matrix adhesion for adherent cell culture. It might be a general strategy for precise evaluation of biomaterials.
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 1520-6882 ; DOI: 10.1021/acs.analchem.8b02653
fulltext: no_fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleMeasurement of Cell-Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture.
creatorMao, Sifeng ; Zhang, Qiang ; Li, Haifang ; Huang, Qiushi ; Khan, Mashooq ; Uchiyama, Katsumi ; Lin, Jin-Ming
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subjectCell Adhesion–Chemistry ; Cell Culture Techniques–Chemistry ; Cell Line–Chemistry ; Coated Materials, Biocompatible–Chemistry ; Equipment Design–Chemistry ; Extracellular Matrix–Chemistry ; Fibronectins–Chemistry ; Glass–Instrumentation ; Humans–Methods ; Lab-On-A-Chip Devices–Methods ; Polylysine–Methods ; Propylamines–Methods ; Silanes–Methods ; Single-Cell Analysis–Methods ; Surface Properties–Methods ; Coated Materials, Biocompatible ; Fibronectins ; Propylamines ; Silanes ; Octadecyltrichlorosilane ; Polylysine ; Amino-Propyl-Triethoxysilane
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descriptionCell adhesion is essential for a cell to maintain its functions, and biomaterials acting as the extracellular matrix (ECM) play a vital role. However, conventional methods for evaluating the functions of biomaterials become insufficient and sometimes incorrect when we give a deeper insight into single-cell research. In this work, we reported a novel methodology for the measurement of cell-matrix adhesion at single-cell resolution that could precisely evaluate the functions of biomaterials for adherent cell culture. A microfludic device, a live single-cell extractor (LSCE), was used for cell extraction. We applied this method to evaluate various modified biomaterials. The results indicated that poly(l-polylysine) (PLL)-coated glass and fibronection (FN)-coated glass slides showed the best biocompatibility for adherent cell culture following by the (3-aminopropyl)triethoxysilane (APTES)-coated glass, while piranha solution treated glass slide and octadecyltrichlorosilane (OTS)-coated glass showed weak biocompatibilities. Furthermore, APTES, PLL, and FN modifications enhanced the cell heterogeneity, while the OTS modification weakened the cell heterogeneity compare to the initial piranha solution treated glass. The method not only clarified the cell-matrix adhesion strength at single-cell resolution but also revealed the influences of biomaterials on cell-matrix adhesion and heterogeneity of cell-matrix adhesion for adherent cell culture. It might be a general strategy for precise evaluation of biomaterials.
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titleMeasurement of Cell-Matrix Adhesion at Single-Cell Resolution for Revealing the Functions of Biomaterials for Adherent Cell Culture.
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