schliessen

Filtern

 

Bibliotheken

Interferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.

Patients with HIV routinely use medicinal cannabinoids to treat neuropathic pain, anxiety, and human immunodeficiency virus (HIV)-associated wasting. However, Δ9-tetrahydrocannabinol (THC), the primary psychoactive cannabinoid in cannabis, suppresses T-cell function and secretion of interferons, bot... Full description

Journal Title: The Journal of pharmacology and experimental therapeutics October 2018, Vol.367(1), pp.49-58
Main Author: Henriquez, Joseph E
Other Authors: Rizzo, Michael D , Crawford, Robert B , Gulick, Peter , Kaminski, Norbert E
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1521-0103 ; DOI: 10.1124/jpet.118.250308
Link: http://search.proquest.com/docview/2073320904/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest2073320904
title: Interferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.
format: Article
creator:
  • Henriquez, Joseph E
  • Rizzo, Michael D
  • Crawford, Robert B
  • Gulick, Peter
  • Kaminski, Norbert E
subjects:
  • Adult–Cytology
  • Aged–Drug Effects
  • Cd4-Positive T-Lymphocytes–Metabolism
  • Cd8-Positive T-Lymphocytes–Cytology
  • Case-Control Studies–Drug Effects
  • Cell Proliferation–Metabolism
  • Dronabinol–Drug Effects
  • Drug Interactions–Pharmacology
  • HIV Infections–Immunology
  • Humans–Pharmacology
  • Interferon-Alpha–Pharmacology
  • Interleukin-7–Drug Effects
  • Lymphocyte Activation–Drug Effects
  • Male–Metabolism
  • Middle Aged–Metabolism
  • Phosphorylation–Drug Effects
  • Receptors, Interleukin-7–Cytology
  • Stat5 Transcription Factor–Drug Effects
  • Signal Transduction–Metabolism
  • T-Lymphocytes
ispartof: The Journal of pharmacology and experimental therapeutics, October 2018, Vol.367(1), pp.49-58
description: Patients with HIV routinely use medicinal cannabinoids to treat neuropathic pain, anxiety, and human immunodeficiency virus (HIV)-associated wasting. However, Δ9-tetrahydrocannabinol (THC), the primary psychoactive cannabinoid in cannabis, suppresses T-cell function and secretion of interferons, both critically important in the antiviral immune response. Interferon-α (IFNα), a key cytokine in T-cell activation and peripheral control of HIV infection, can potentiate responsiveness to interleukin-7 (IL-7), a crucial homeostatic cytokine for peripheral T-cell maintenance. The objective of this investigation was to compare the response of T cells to stimulation by IFNα and IL-7 in T cells from healthy and HIV+ donors in the absence and presence of THC. To compare T-cell responses between healthy and HIV+ donors signaling through IFNα receptor, IFNα-induced expression of IL-7α receptor (IL-7Rα), cognate signaling through IL-7R, and on IL-7-mediated T-cell proliferation were measured by flow cytometry and real-time quantitative polymerase chain reaction. CD8+ T cells from HIV+ donors showed a diminished response to IFNα-induced phosphorylated signal transducer and activator of transcription-1 activation compared with CD8+ T cells from healthy donors, whereas CD4+ T cells from HIV+ donors and healthy donors were comparable. Treatment with IFNα promoted IL-7R expression and potentiated IL-7-induced STAT5 phosphorylation to augment IL-7-mediated proliferation by T cells from healthy and HIV+ donors. Finally, HIV+ donors exhibited reduced sensitivity to THC-mediated suppression by IFNα- and IL-7-mediated stimulation compared with healthy donors. These results further support THC as being immune suppressive while identifying putatively beneficial aspects of cannabinoid-based therapies in HIV+ patients.
language: eng
source:
identifier: E-ISSN: 1521-0103 ; DOI: 10.1124/jpet.118.250308
fulltext: no_fulltext
issn:
  • 15210103
  • 1521-0103
url: Link


@attributes
ID1886284890
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid2073320904
sourceidproquest
recordidTN_proquest2073320904
sourcesystemPC
pqid2073320904
display
typearticle
titleInterferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.
creatorHenriquez, Joseph E ; Rizzo, Michael D ; Crawford, Robert B ; Gulick, Peter ; Kaminski, Norbert E
contributorHenriquez, Joseph E (correspondence author) ; Henriquez, Joseph E (record owner)
ispartofThe Journal of pharmacology and experimental therapeutics, October 2018, Vol.367(1), pp.49-58
identifierE-ISSN: 1521-0103 ; DOI: 10.1124/jpet.118.250308
subjectAdult–Cytology ; Aged–Drug Effects ; Cd4-Positive T-Lymphocytes–Metabolism ; Cd8-Positive T-Lymphocytes–Cytology ; Case-Control Studies–Drug Effects ; Cell Proliferation–Metabolism ; Dronabinol–Drug Effects ; Drug Interactions–Pharmacology ; HIV Infections–Immunology ; Humans–Pharmacology ; Interferon-Alpha–Pharmacology ; Interleukin-7–Drug Effects ; Lymphocyte Activation–Drug Effects ; Male–Metabolism ; Middle Aged–Metabolism ; Phosphorylation–Drug Effects ; Receptors, Interleukin-7–Cytology ; Stat5 Transcription Factor–Drug Effects ; Signal Transduction–Metabolism ; T-Lymphocytes
languageeng
source
descriptionPatients with HIV routinely use medicinal cannabinoids to treat neuropathic pain, anxiety, and human immunodeficiency virus (HIV)-associated wasting. However, Δ9-tetrahydrocannabinol (THC), the primary psychoactive cannabinoid in cannabis, suppresses T-cell function and secretion of interferons, both critically important in the antiviral immune response. Interferon-α (IFNα), a key cytokine in T-cell activation and peripheral control of HIV infection, can potentiate responsiveness to interleukin-7 (IL-7), a crucial homeostatic cytokine for peripheral T-cell maintenance. The objective of this investigation was to compare the response of T cells to stimulation by IFNα and IL-7 in T cells from healthy and HIV+ donors in the absence and presence of THC. To compare T-cell responses between healthy and HIV+ donors signaling through IFNα receptor, IFNα-induced expression of IL-7α receptor (IL-7Rα), cognate signaling through IL-7R, and on IL-7-mediated T-cell proliferation were measured by flow cytometry and real-time quantitative polymerase chain reaction. CD8+ T cells from HIV+ donors showed a diminished response to IFNα-induced phosphorylated signal transducer and activator of transcription-1 activation compared with CD8+ T cells from healthy donors, whereas CD4+ T cells from HIV+ donors and healthy donors were comparable. Treatment with IFNα promoted IL-7R expression and potentiated IL-7-induced STAT5 phosphorylation to augment IL-7-mediated proliferation by T cells from healthy and HIV+ donors. Finally, HIV+ donors exhibited reduced sensitivity to THC-mediated suppression by IFNα- and IL-7-mediated stimulation compared with healthy donors. These results further support THC as being immune suppressive while identifying putatively beneficial aspects of cannabinoid-based therapies in HIV+ patients.
version3
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/2073320904/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Henriquez, Joseph E
1Rizzo, Michael D
2Crawford, Robert B
3Gulick, Peter
4Kaminski, Norbert E
titleInterferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.
subject
0Adult–Cytology
1Aged–Drug Effects
2Cd4-Positive T-Lymphocytes–Metabolism
3Cd8-Positive T-Lymphocytes–Cytology
4Case-Control Studies–Drug Effects
5Cell Proliferation–Metabolism
6Dronabinol–Drug Effects
7Drug Interactions–Pharmacology
8HIV Infections–Immunology
9Humans–Pharmacology
10Interferon-Alpha–Pharmacology...
general
0English
110.1124/jpet.118.250308
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest2073320904
issn
015210103
11521-0103
rsrctypearticle
creationdate2018
addtitleThe Journal of pharmacology and experimental therapeutics
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorHenriquez, Joseph E
startdate20181001
enddate20181001
citationpf 49 pt 58 vol 367 issue 1
lsr30VSR-Enriched:[pqid, issn, description]
sort
titleInterferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.
authorHenriquez, Joseph E ; Rizzo, Michael D ; Crawford, Robert B ; Gulick, Peter ; Kaminski, Norbert E
creationdate20181001
lso0120181001
facets
frbrgroupid2296714351327718888
frbrtype5
newrecords20181218
languageeng
creationdate2018
topic
0Adult–Cytology
1Aged–Drug Effects
2Cd4-Positive T-Lymphocytes–Metabolism
3Cd8-Positive T-Lymphocytes–Cytology
4Case-Control Studies–Drug Effects
5Cell Proliferation–Metabolism
6Dronabinol–Drug Effects
7Drug Interactions–Pharmacology
8HIV Infections–Immunology
9Humans–Pharmacology
10Interferon-Alpha–Pharmacology...
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Henriquez, Joseph E
1Rizzo, Michael D
2Crawford, Robert B
3Gulick, Peter
4Kaminski, Norbert E
jtitleJournal of pharmacology and experimental therapeutics
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
aulast
0Henriquez
1Rizzo
2Crawford
3Gulick
4Kaminski
aufirst
0Joseph E
1Michael D
2Robert B
3Peter
4Norbert E
au
0Henriquez, Joseph E
1Rizzo, Michael D
2Crawford, Robert B
3Gulick, Peter
4Kaminski, Norbert E
addauHenriquez, Joseph E
atitleInterferon-α-Mediated Activation of T Cells from Healthy and HIV-Infected Individuals Is Suppressed by Δ9-Tetrahydrocannabinol.
jtitleThe Journal of pharmacology and experimental therapeutics
risdate20181001
volume367
issue1
spage49
epage58
pages49-58
eissn1521-0103
formatjournal
genrearticle
ristypeJOUR
doi10.1124/jpet.118.250308
urlhttp://search.proquest.com/docview/2073320904/
issn00223565
date2018-10-01