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Promoter-mediated diversification of transcriptional bursting dynamics following gene duplication.

During the evolution of gene families, functional diversification of proteins often follows gene duplication. However, many gene families expand while preserving protein sequence. Why do cells maintain multiple copies of the same gene? Here we have addressed this question for an actin family with 17... Full description

Journal Title: Proceedings of the National Academy of Sciences of the United States of America August 14, 2018, Vol.115(33), pp.8364-8369
Main Author: Tunnacliffe, Edward
Other Authors: Corrigan, Adam M , Chubb, Jonathan R
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1800943115
Link: http://search.proquest.com/docview/2080840309/?pq-origsite=primo
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title: Promoter-mediated diversification of transcriptional bursting dynamics following gene duplication.
format: Article
creator:
  • Tunnacliffe, Edward
  • Corrigan, Adam M
  • Chubb, Jonathan R
subjects:
  • Actins–Genetics
  • Cell Line–Genetics
  • Dictyostelium–Genetics
  • Gene Duplication–Genetics
  • Gene Expression Regulation–Genetics
  • Promoter Regions, Genetic–Genetics
  • Transcription, Genetic–Genetics
  • Actins
  • Dictyostelium
  • Gene Family
  • Single-Cell Transcriptomics
  • Stochastic Gene Expression
  • Transcriptional Bursting
ispartof: Proceedings of the National Academy of Sciences of the United States of America, August 14, 2018, Vol.115(33), pp.8364-8369
description: During the evolution of gene families, functional diversification of proteins often follows gene duplication. However, many gene families expand while preserving protein sequence. Why do cells maintain multiple copies of the same gene? Here we have addressed this question for an actin family with 17 genes encoding an identical protein. The genes have divergent flanking regions and are scattered throughout the genome. Surprisingly, almost the entire family showed similar developmental expression profiles, with their expression also strongly coupled in single cells. Using live cell imaging, we show that differences in gene expression were apparent over shorter timescales, with family members displaying different transcriptional bursting dynamics. Strong "bursty" behaviors contrasted steady, more continuous activity, indicating different regulatory inputs to individual actin genes. To determine the sources of these different dynamic behaviors, we reciprocally exchanged the upstream regulatory...
language: eng
source:
identifier: E-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1800943115
fulltext: fulltext
issn:
  • 10916490
  • 1091-6490
url: Link


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titlePromoter-mediated diversification of transcriptional bursting dynamics following gene duplication.
creatorTunnacliffe, Edward ; Corrigan, Adam M ; Chubb, Jonathan R
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ispartofProceedings of the National Academy of Sciences of the United States of America, August 14, 2018, Vol.115(33), pp.8364-8369
identifierE-ISSN: 1091-6490 ; DOI: 10.1073/pnas.1800943115
subjectActins–Genetics ; Cell Line–Genetics ; Dictyostelium–Genetics ; Gene Duplication–Genetics ; Gene Expression Regulation–Genetics ; Promoter Regions, Genetic–Genetics ; Transcription, Genetic–Genetics ; Actins ; Dictyostelium ; Gene Family ; Single-Cell Transcriptomics ; Stochastic Gene Expression ; Transcriptional Bursting
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descriptionDuring the evolution of gene families, functional diversification of proteins often follows gene duplication. However, many gene families expand while preserving protein sequence. Why do cells maintain multiple copies of the same gene? Here we have addressed this question for an actin family with 17 genes encoding an identical protein. The genes have divergent flanking regions and are scattered throughout the genome. Surprisingly, almost the entire family showed similar developmental expression profiles, with their expression also strongly coupled in single cells. Using live cell imaging, we show that differences in gene expression were apparent over shorter timescales, with family members displaying different transcriptional bursting dynamics. Strong "bursty" behaviors contrasted steady, more continuous activity, indicating different regulatory inputs to individual actin genes. To determine the sources of these different dynamic behaviors, we reciprocally exchanged the upstream regulatory...
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