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MicroRNA-486 Alleviates Hypoxia-Induced Damage in H9c2 Cells by Targeting NDRG2 to Inactivate JNK/C-Jun and NF-κB Signaling Pathways.

BACKGROUND/AIMSAcute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of mi... Full description

Journal Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology biochemistry, and pharmacology, 2018, Vol.48(6), pp.2483-2492
Main Author: Zhang, Xinling
Other Authors: Zhang, Chunxiang , Wang, Nan , Li, Yan , Zhang, Debing , Li, Qiang
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1421-9778 ; DOI: 10.1159/000492686
Link: http://search.proquest.com/docview/2090321157/?pq-origsite=primo
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title: MicroRNA-486 Alleviates Hypoxia-Induced Damage in H9c2 Cells by Targeting NDRG2 to Inactivate JNK/C-Jun and NF-κB Signaling Pathways.
format: Article
creator:
  • Zhang, Xinling
  • Zhang, Chunxiang
  • Wang, Nan
  • Li, Yan
  • Zhang, Debing
  • Li, Qiang
subjects:
  • Animals–Metabolism
  • Antagomirs–Metabolism
  • Caspase 3–Metabolism
  • Caspase 9–Metabolism
  • Cell Hypoxia–Antagonists & Inhibitors
  • Cell Line–Genetics
  • Cell Movement–Metabolism
  • Jnk Mitogen-Activated Protein Kinases–Metabolism
  • Micrornas–Antagonists & Inhibitors
  • Nf-Kappa B–Genetics
  • Nerve Tissue Proteins–Metabolism
  • RNA Interference–Metabolism
  • RNA, Small Interfering–Metabolism
  • Rats–Metabolism
  • Signal Transduction–Metabolism
  • Bcl-2-Associated X Protein–Metabolism
  • Antagomirs
  • Mirn486 Microrna, Rat
ispartof: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2018, Vol.48(6), pp.2483-2492
description: BACKGROUND/AIMSAcute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODSH9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC). Effects of miR-486 and/or NDRG2 dysregulation on hypoxia-induced myocardial injury in H9c2 cells were investigated by evaluating cell viability, migration, invasion and apoptosis using Cell Counting Kit-8 (CCK-8), transwell assay, flow cytometry, respectively. The proteins expression and RNA expression were detected by western blot and quantitative real time polymerase chain reaction (qRT-PCR),...
language: eng
source:
identifier: E-ISSN: 1421-9778 ; DOI: 10.1159/000492686
fulltext: fulltext
issn:
  • 14219778
  • 1421-9778
url: Link


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titleMicroRNA-486 Alleviates Hypoxia-Induced Damage in H9c2 Cells by Targeting NDRG2 to Inactivate JNK/C-Jun and NF-κB Signaling Pathways.
creatorZhang, Xinling ; Zhang, Chunxiang ; Wang, Nan ; Li, Yan ; Zhang, Debing ; Li, Qiang
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ispartofCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2018, Vol.48(6), pp.2483-2492
identifierE-ISSN: 1421-9778 ; DOI: 10.1159/000492686
subjectAnimals–Metabolism ; Antagomirs–Metabolism ; Caspase 3–Metabolism ; Caspase 9–Metabolism ; Cell Hypoxia–Antagonists & Inhibitors ; Cell Line–Genetics ; Cell Movement–Metabolism ; Jnk Mitogen-Activated Protein Kinases–Metabolism ; Micrornas–Antagonists & Inhibitors ; Nf-Kappa B–Genetics ; Nerve Tissue Proteins–Metabolism ; RNA Interference–Metabolism ; RNA, Small Interfering–Metabolism ; Rats–Metabolism ; Signal Transduction–Metabolism ; Bcl-2-Associated X Protein–Metabolism ; Antagomirs ; Mirn486 Microrna, Rat
descriptionBACKGROUND/AIMSAcute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODSH9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC). Effects of miR-486 and/or NDRG2 dysregulation on hypoxia-induced myocardial injury in H9c2 cells were investigated by evaluating cell viability, migration, invasion and apoptosis using Cell Counting Kit-8 (CCK-8), transwell assay, flow cytometry, respectively. The proteins expression and RNA expression were detected by western blot and quantitative real time polymerase chain reaction (qRT-PCR),...
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titleMicroRNA-486 Alleviates Hypoxia-Induced Damage in H9c2 Cells by Targeting NDRG2 to Inactivate JNK/C-Jun and NF-κB Signaling Pathways.
descriptionBACKGROUND/AIMSAcute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODSH9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC). Effects of miR-486 and/or NDRG2 dysregulation on hypoxia-induced myocardial injury in H9c2 cells were investigated by evaluating cell viability, migration, invasion and apoptosis using Cell Counting Kit-8 (CCK-8), transwell assay, flow cytometry, respectively. The proteins expression and RNA expression were detected by western blot and quantitative real time polymerase chain reaction (qRT-PCR),...
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titleMicroRNA-486 Alleviates Hypoxia-Induced Damage in H9c2 Cells by Targeting NDRG2 to Inactivate JNK/C-Jun and NF-κB Signaling Pathways.
authorZhang, Xinling ; Zhang, Chunxiang ; Wang, Nan ; Li, Yan ; Zhang, Debing ; Li, Qiang
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abstractBACKGROUND/AIMSAcute myocardial infarction is a serious disease with high morbidity and mortality. microRNAs (miRNAs) have been proved to play an important role in modulating myocardial ischemia and reperfusion injury. Hence, in this study, we constructed H9c2 cell model to elucidate the roles of microRNA-486 (miR-486) in preventing hypoxia-induced damage in H9c2 cells. METHODSH9c2 cells were cultured in hypoxic incubator with 1% O2 to simulate hypoxia and/or transfected with miR-486 mimic, scramble, anti-miR-486, si-N-myc downstream-regulated gene 2 (NDRG2) and their corresponding negative controls (NC). Effects of miR-486 and/or NDRG2 dysregulation on hypoxia-induced myocardial injury in H9c2 cells were investigated by evaluating cell viability, migration, invasion and apoptosis using Cell Counting Kit-8 (CCK-8), transwell assay, flow cytometry, respectively. The proteins expression and RNA expression were detected by western blot and quantitative real time polymerase chain reaction (qRT-PCR),...
doi10.1159/000492686
urlhttp://search.proquest.com/docview/2090321157/
issn10158987
date2018-01-01