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Antimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics

Aim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were col... Full description

Journal Title: Veterinary World Aug 2018, Vol.11(8), pp.1082-1088
Main Author: Amer, Mohamed
Other Authors: Mekky, Hoda , Amer, Aziza , Fedawy, Hanaa
Format: Electronic Article Electronic Article
Language: English
Subjects:
Amp
ID: ISSN: 09728988 ; E-ISSN: 22310916 ; DOI: 10.14202/vetworld.2018.1082-1088
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title: Antimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics
format: Article
creator:
  • Amer, Mohamed
  • Mekky, Hoda
  • Amer, Aziza
  • Fedawy, Hanaa
subjects:
  • Escherichia Coli
  • Clindamycin
  • Liver
  • Kidneys
  • Pathogenicity
  • Phenotypes
  • Drug Resistance
  • Erythromycin
  • Antimicrobial Resistance
  • Streptomycin
  • Antibiotics
  • Amides
  • Genotypes
  • Antiinfectives and Antibacterials
  • Oxacillin
  • Bacteria
  • Farms
  • Poultry
  • Chloramphenicol
  • Agglutination
  • Gene Expression
  • Enrofloxacin
  • Escherichia Coli
  • Trimethoprim
  • Gene Expression
  • Antibiotics
  • Amp
  • Aminoglycoside Antibiotics
  • Gentamicin
  • Gene Expression
  • Liver
  • Chickens
  • Antibiotics
  • Kanamycin
  • Resistance Factors
  • Lung
  • Oxytetracycline
  • Genes
  • Polymerase Chain Reaction
  • E Coli
  • Cefotaxime
  • Farms
  • Kidneys
  • Multidrug Resistance
  • Escherichia Coli
  • Chloromycetin
  • Erythromycin
  • Drug Resistance
  • Pathogens
  • Birds
  • Agglutination
  • Polymerase Chain Reaction
  • Antibiotics
  • Ampicillin
  • Diffusion
  • Oxytetracycline
  • Ampicillin
  • Antimicrobial Agents
  • Diffusion Rate
  • Liver
  • Poultry
  • Antibiotic Resistance Genes
  • Broiler
  • Escherichia Coli
  • Isolation
ispartof: Veterinary World, Aug 2018, Vol.11(8), pp.1082-1088
description: Aim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were collected from 18 to 34 days old clinically diseased broiler from 40 broiler farms (3-5 birds/farm) reared in Giza and Kaluobaia Governorates for the isolation of pathogenic E. coli. Various E. coli isolates were tested for the pathogenicity based on Congo red (CR) dye binding assay. The obtained CR-positive E. coli isolates were subjected to serological identification using slide agglutination test. Disc diffusion test was used to study the sensitivity pattern of E. coli isolates to available 12 antibiotics. Polymerase chain reaction was performed for the detection of antimicrobial resistance genes in the studied pathogenic E. coli isolates. Results: The results revealed that 56 samples (35 %) were positive for E. coli. The results of the CR assay indicates that 20 isolates of 56 (35.7%) were positive and 36 isolates (64.3%) were negative. Identified E. coli serotypes of CR-positive isolates were 1 (O24), 2 (O44), 2 (O55), 5 (O78), 2 (O86), 1 (124), 3 (O127), 1 (O158), and 3 untyped. Resistance rate in disc diffusion test was 85% to oxytetracycline and kanamycin; 80% to ampicillin (AMP), clindamycin, and streptomycin (S); 75% to enrofloxacin; 65% to chloramphenicol; 55% to cefotaxime and gentamicin (CN); 45% to trimethoprim+sulfamethoxazole; 35% to erythromycin (ERI); and 30% to oxacillin. All strains are multidrug-resistant (MDR). Antibacterial resistance genes CITM, ere, aac (3)-(IV), tet(A), tet(B), dfr(A1), and aad(A1) were detected in 14 (70%), 12 (60%), 12 (60%), 8 (40%), 11 (55%), 8 (40%), and 9 (45%) of tested 20 isolates, respectively. Multidrug resistance was detected in the form of resistance to 42%-83.3% of tested 12 antibiotics. Three isolates (15%) of 20 tested isolates showed a relationship between phenotype and genotype and 17 (85%) showed irregular relation. Strains are sensitive and show resistant gene (P-G+) presented in three isolates for AMP (beta-lactam), one for ERI (Macrolide), as well as five isolates for trimethoprim (pyrimidine inhibitor). E. coli isolates had resistance and lacked gene (P+ G-) reported meanly in one isolate for CN (aminoglycoside), two isolates for tetracycline, four isolates for ERI, seven isolates for trimethoprim, an
language: eng
source:
identifier: ISSN: 09728988 ; E-ISSN: 22310916 ; DOI: 10.14202/vetworld.2018.1082-1088
fulltext: fulltext_linktorsrc
issn:
  • 09728988
  • 0972-8988
  • 22310916
  • 2231-0916
url: Link


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titleAntimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics
creatorAmer, Mohamed ; Mekky, Hoda ; Amer, Aziza ; Fedawy, Hanaa
ispartofVeterinary World, Aug 2018, Vol.11(8), pp.1082-1088
identifier
subjectEscherichia Coli ; Clindamycin ; Liver ; Kidneys ; Pathogenicity ; Phenotypes ; Drug Resistance ; Erythromycin ; Antimicrobial Resistance ; Streptomycin ; Antibiotics ; Amides ; Genotypes ; Antiinfectives and Antibacterials ; Oxacillin ; Bacteria ; Farms ; Poultry ; Chloramphenicol ; Agglutination ; Gene Expression ; Enrofloxacin ; Escherichia Coli ; Trimethoprim ; Gene Expression ; Antibiotics ; Amp ; Aminoglycoside Antibiotics ; Gentamicin ; Gene Expression ; Liver ; Chickens ; Antibiotics ; Kanamycin ; Resistance Factors ; Lung ; Oxytetracycline ; Genes ; Polymerase Chain Reaction ; E Coli ; Cefotaxime ; Farms ; Kidneys ; Multidrug Resistance ; Escherichia Coli ; Chloromycetin ; Erythromycin ; Drug Resistance ; Pathogens ; Birds ; Agglutination ; Polymerase Chain Reaction ; Antibiotics ; Ampicillin ; Diffusion ; Oxytetracycline ; Ampicillin ; Antimicrobial Agents ; Diffusion Rate ; Liver ; Poultry ; Antibiotic Resistance Genes ; Broiler ; Escherichia Coli ; Isolation
descriptionAim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were collected from 18 to 34 days old clinically diseased broiler from 40 broiler farms (3-5 birds/farm) reared in Giza and Kaluobaia Governorates for the isolation of pathogenic E. coli. Various E. coli isolates were tested for the pathogenicity based on Congo red (CR) dye binding assay. The obtained CR-positive E. coli isolates were subjected to serological identification using slide agglutination test. Disc diffusion test was used to study the sensitivity pattern of E. coli isolates to available 12 antibiotics. Polymerase chain reaction was performed for the detection of antimicrobial resistance genes in the studied pathogenic E. coli isolates. Results: The results revealed that 56 samples (35 %) were positive for E. coli. The results of the CR assay indicates that 20 isolates of 56 (35.7%) were positive and 36 isolates (64.3%) were negative. Identified E. coli serotypes of CR-positive isolates were 1 (O24), 2 (O44), 2 (O55), 5 (O78), 2 (O86), 1 (124), 3 (O127), 1 (O158), and 3 untyped. Resistance rate in disc diffusion test was 85% to oxytetracycline and kanamycin; 80% to ampicillin (AMP), clindamycin, and streptomycin (S); 75% to enrofloxacin; 65% to chloramphenicol; 55% to cefotaxime and gentamicin (CN); 45% to trimethoprim+sulfamethoxazole; 35% to erythromycin (ERI); and 30% to oxacillin. All strains are multidrug-resistant (MDR). Antibacterial resistance genes CITM, ere, aac (3)-(IV), tet(A), tet(B), dfr(A1), and aad(A1) were detected in 14 (70%), 12 (60%), 12 (60%), 8 (40%), 11 (55%), 8 (40%), and 9 (45%) of tested 20 isolates, respectively. Multidrug resistance was detected in the form of resistance to 42%-83.3% of tested 12 antibiotics. Three isolates (15%) of 20 tested isolates showed a relationship between phenotype and genotype and 17 (85%) showed irregular relation. Strains are sensitive and show resistant gene (P-G+) presented in three isolates for AMP (beta-lactam), one for ERI (Macrolide), as well as five isolates for trimethoprim (pyrimidine inhibitor). E. coli isolates had resistance and lacked gene (P+ G-) reported meanly in one isolate for CN (aminoglycoside), two isolates for tetracycline, four isolates for ERI, seven isolates for trimethoprim, and eight isolates for S (aminoglycoside). Conclusions: The study demonstrates that E. coli is still a major pathogen responsible for disease conditions in broiler. E. coli isolates are pathogenic and MDR. Responsible gene was detected for six antibiotics in most of the isolates, but some do not show gene expression, this may be due to few numbers of resistance genes tested or other resistance factors not included in this study.
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titleAntimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics
descriptionAim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were collected from 18 to 34 days old clinically diseased broiler from 40 broiler farms (3-5 birds/farm) reared in Giza and Kaluobaia Governorates for the isolation of pathogenic E. coli. Various E. coli isolates were tested for the pathogenicity based on Congo red (CR) dye binding assay. The obtained CR-positive E. coli isolates were subjected to serological identification using slide agglutination test. Disc diffusion test was used to study the sensitivity pattern of E. coli isolates to available 12 antibiotics. Polymerase chain reaction was performed for the detection of antimicrobial resistance genes in the studied pathogenic E. coli isolates. Results: The results revealed that 56 samples (35 %) were positive for E. coli. The results of the CR assay indicates that 20 isolates of 56 (35.7%) were positive and 36 isolates (64.3%) were negative. Identified E. coli serotypes of CR-positive isolates were 1 (O24), 2 (O44), 2 (O55), 5 (O78), 2 (O86), 1 (124), 3 (O127), 1 (O158), and 3 untyped. Resistance rate in disc diffusion test was 85% to oxytetracycline and kanamycin; 80% to ampicillin (AMP), clindamycin, and streptomycin (S); 75% to enrofloxacin; 65% to chloramphenicol; 55% to cefotaxime and gentamicin (CN); 45% to trimethoprim+sulfamethoxazole; 35% to erythromycin (ERI); and 30% to oxacillin. All strains are multidrug-resistant (MDR). Antibacterial resistance genes CITM, ere, aac (3)-(IV), tet(A), tet(B), dfr(A1), and aad(A1) were detected in 14 (70%), 12 (60%), 12 (60%), 8 (40%), 11 (55%), 8 (40%), and 9 (45%) of tested 20 isolates, respectively. Multidrug resistance was detected in the form of resistance to 42%-83.3% of tested 12 antibiotics. Three isolates (15%) of 20 tested isolates showed a relationship between phenotype and genotype and 17 (85%) showed irregular relation. Strains are sensitive and show resistant gene (P-G+) presented in three isolates for AMP (beta-lactam), one for ERI (Macrolide), as well as five isolates for trimethoprim (pyrimidine inhibitor). E. coli isolates had resistance and lacked gene (P+ G-) reported meanly in one isolate for CN (aminoglycoside), two isolates for tetracycline, four isolates for ERI, seven isolates for trimethoprim, and eight isolates for S (aminoglycoside). Conclusions: The study demonstrates that E. coli is still a major pathogen responsible for disease conditions in broiler. E. coli isolates are pathogenic and MDR. Responsible gene was detected for six antibiotics in most of the isolates, but some do not show gene expression, this may be due to few numbers of resistance genes tested or other resistance factors not included in this study.
subject
0Escherichia Coli
1Clindamycin
2Liver
3Kidneys
4Pathogenicity
5Phenotypes
6Drug Resistance
7Erythromycin
8Antimicrobial Resistance
9Streptomycin
10Antibiotics
11Amides
12Genotypes
13Antiinfectives and Antibacterials
14Oxacillin
15Bacteria
16Farms
17Poultry
18Chloramphenicol
19Agglutination
20Gene Expression
21Enrofloxacin
22Trimethoprim
23Amp
24Aminoglycoside Antibiotics
25Gentamicin
26Chickens
27Kanamycin
28Resistance Factors
29Lung
30Oxytetracycline
31Genes
32Polymerase Chain Reaction
33E Coli
34Cefotaxime
35Multidrug Resistance
36Chloromycetin
37Pathogens
38Birds
39Ampicillin
40Diffusion
41Antimicrobial Agents
42Diffusion Rate
43Antibiotic Resistance Genes
44Broiler
45Isolation
46antibiotic resistance genes
47broiler
48Escherichia coli
49isolation
general
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titleAntimicrobial resistance genes in pathogenic Escherichia coli isolated from diseased broiler chickens in Egypt and their relationship with the phenotypic resistance characteristics
authorAmer, Mohamed ; Mekky, Hoda ; Amer, Aziza ; Fedawy, Hanaa
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3Kidneys
4Pathogenicity
5Phenotypes
6Drug Resistance
7Erythromycin
8Antimicrobial Resistance
9Streptomycin
10Antibiotics
11Amides
12Genotypes
13Antiinfectives and Antibacterials
14Oxacillin
15Bacteria
16Farms
17Poultry
18Chloramphenicol
19Agglutination
20Gene Expression
21Enrofloxacin
22Trimethoprim
23Amp
24Aminoglycoside Antibiotics
25Gentamicin
26Chickens
27Kanamycin
28Resistance Factors
29Lung
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34Cefotaxime
35Multidrug Resistance
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37Pathogens
38Birds
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43Antibiotic Resistance Genes
44Broiler
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abstractAim: The aim of this study was to determine the relationship between phenotypic resistance and genotypic resistance of isolated serotyped pathogenic Escherichia coli isolates from the clinically diseased broiler. Materials and Methods: A total of 160 samples (heart, liver, kidney, and lung) were collected from 18 to 34 days old clinically diseased broiler from 40 broiler farms (3-5 birds/farm) reared in Giza and Kaluobaia Governorates for the isolation of pathogenic E. coli. Various E. coli isolates were tested for the pathogenicity based on Congo red (CR) dye binding assay. The obtained CR-positive E. coli isolates were subjected to serological identification using slide agglutination test. Disc diffusion test was used to study the sensitivity pattern of E. coli isolates to available 12 antibiotics. Polymerase chain reaction was performed for the detection of antimicrobial resistance genes in the studied pathogenic E. coli isolates. Results: The results revealed that 56 samples (35 %) were positive for E. coli. The results of the CR assay indicates that 20 isolates of 56 (35.7%) were positive and 36 isolates (64.3%) were negative. Identified E. coli serotypes of CR-positive isolates were 1 (O24), 2 (O44), 2 (O55), 5 (O78), 2 (O86), 1 (124), 3 (O127), 1 (O158), and 3 untyped. Resistance rate in disc diffusion test was 85% to oxytetracycline and kanamycin; 80% to ampicillin (AMP), clindamycin, and streptomycin (S); 75% to enrofloxacin; 65% to chloramphenicol; 55% to cefotaxime and gentamicin (CN); 45% to trimethoprim+sulfamethoxazole; 35% to erythromycin (ERI); and 30% to oxacillin. All strains are multidrug-resistant (MDR). Antibacterial resistance genes CITM, ere, aac (3)-(IV), tet(A), tet(B), dfr(A1), and aad(A1) were detected in 14 (70%), 12 (60%), 12 (60%), 8 (40%), 11 (55%), 8 (40%), and 9 (45%) of tested 20 isolates, respectively. Multidrug resistance was detected in the form of resistance to 42%-83.3% of tested 12 antibiotics. Three isolates (15%) of 20 tested isolates showed a relationship between phenotype and genotype and 17 (85%) showed irregular relation. Strains are sensitive and show resistant gene (P-G+) presented in three isolates for AMP (beta-lactam), one for ERI (Macrolide), as well as five isolates for trimethoprim (pyrimidine inhibitor). E. coli isolates had resistance and lacked gene (P+ G-) reported meanly in one isolate for CN (aminoglycoside), two isolates for tetracycline, four isolates for ERI, seven isolates for trimethoprim, and eight isolates for S (aminoglycoside). Conclusions: The study demonstrates that E. coli is still a major pathogen responsible for disease conditions in broiler. E. coli isolates are pathogenic and MDR. Responsible gene was detected for six antibiotics in most of the isolates, but some do not show gene expression, this may be due to few numbers of resistance genes tested or other resistance factors not included in this study.
copRajkot
pubVeterinary World
doi10.14202/vetworld.2018.1082-1088
urlhttp://search.proquest.com/docview/2114284132/
oafree_for_read
date2018-08-01