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Dishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.

Frizzleds (FZDs) are receptors for secreted lipoglycoproteins of the Wingless/Int-1(WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are G protein-coupled receptors (GPCRs), which are well known to be regulated by phosphorylation, leading to specific... Full description

Journal Title: The Journal of biological chemistry November 30, 2018, Vol.293(48), pp.18477-18493
Main Author: Strakova, Katerina
Other Authors: Kowalski-Jahn, Maria , Gybel, Tomas , Valnohova, Jana , Dhople, Vishnu M , Harnos, Jakub , Bernatik, Ondrej , Ganji, Ranjani Sri , Zdrahal, Zbynek , Mulder, Jan , Lindskog, Cecilia , Bryja, Vitezslav , Schulte, Gunnar
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1083-351X ; DOI: 10.1074/jbc.RA118.004656
Link: http://search.proquest.com/docview/2119920039/?pq-origsite=primo
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title: Dishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.
format: Article
creator:
  • Strakova, Katerina
  • Kowalski-Jahn, Maria
  • Gybel, Tomas
  • Valnohova, Jana
  • Dhople, Vishnu M
  • Harnos, Jakub
  • Bernatik, Ondrej
  • Ganji, Ranjani Sri
  • Zdrahal, Zbynek
  • Mulder, Jan
  • Lindskog, Cecilia
  • Bryja, Vitezslav
  • Schulte, Gunnar
subjects:
  • Amino Acid Sequence–Immunology
  • Antibodies–Metabolism
  • Casein Kinase I–Metabolism
  • Cell Membrane–Chemistry
  • Dishevelled Proteins–Physiology
  • Epithelium–Metabolism
  • Fallopian Tubes–Metabolism
  • Female–Chemistry
  • Frizzled Receptors–Metabolism
  • Hek293 Cells–Immunology
  • Humans–Metabolism
  • Mass Spectrometry–Metabolism
  • Phosphoproteins–Metabolism
  • Phosphorylation–Metabolism
  • Serine–Metabolism
  • Signal Transduction–Metabolism
  • Antibodies
  • Dishevelled Proteins
  • Fzd6 Protein, Human
  • Frizzled Receptors
ispartof: The Journal of biological chemistry, November 30, 2018, Vol.293(48), pp.18477-18493
description: Frizzleds (FZDs) are receptors for secreted lipoglycoproteins of the Wingless/Int-1(WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are G protein-coupled receptors (GPCRs), which are well known to be regulated by phosphorylation, leading to specific downstream signaling or receptor desensitization. The role and underlying mechanisms of FZD phosphorylation remain largely unexplored. Here, we investigated the phosphorylation of human FZD(6). Using MS analysis and a phospho-state- and -site-specific antibody, we found that Ser-648, located in the FZD(6) C terminus, is efficiently phosphorylated by casein kinase 1 is an element of (CK1 is an element of) and that this phosphorylation requires the scaffolding protein Dishevelled (DVL). In an overexpression system, DVL1, -2, and -3 promoted CK1-mediated FZD(6) phosphorylation on Ser-648. This DVL activity required an intact DEP domain and FZD-mediated recruitment of this domain to the cell membrane....
language: eng
source:
identifier: E-ISSN: 1083-351X ; DOI: 10.1074/jbc.RA118.004656
fulltext: fulltext
issn:
  • 1083351X
  • 1083-351X
url: Link


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titleDishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.
creatorStrakova, Katerina ; Kowalski-Jahn, Maria ; Gybel, Tomas ; Valnohova, Jana ; Dhople, Vishnu M ; Harnos, Jakub ; Bernatik, Ondrej ; Ganji, Ranjani Sri ; Zdrahal, Zbynek ; Mulder, Jan ; Lindskog, Cecilia ; Bryja, Vitezslav ; Schulte, Gunnar
contributorStrakova, Katerina (correspondence author) ; Strakova, Katerina (record owner)
ispartofThe Journal of biological chemistry, November 30, 2018, Vol.293(48), pp.18477-18493
identifierE-ISSN: 1083-351X ; DOI: 10.1074/jbc.RA118.004656
subjectAmino Acid Sequence–Immunology ; Antibodies–Metabolism ; Casein Kinase I–Metabolism ; Cell Membrane–Chemistry ; Dishevelled Proteins–Physiology ; Epithelium–Metabolism ; Fallopian Tubes–Metabolism ; Female–Chemistry ; Frizzled Receptors–Metabolism ; Hek293 Cells–Immunology ; Humans–Metabolism ; Mass Spectrometry–Metabolism ; Phosphoproteins–Metabolism ; Phosphorylation–Metabolism ; Serine–Metabolism ; Signal Transduction–Metabolism ; Antibodies ; Dishevelled Proteins ; Fzd6 Protein, Human ; Frizzled Receptors
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descriptionFrizzleds (FZDs) are receptors for secreted lipoglycoproteins of the Wingless/Int-1(WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are G protein-coupled receptors (GPCRs), which are well known to be regulated by phosphorylation, leading to specific downstream signaling or receptor desensitization. The role and underlying mechanisms of FZD phosphorylation remain largely unexplored. Here, we investigated the phosphorylation of human FZD(6). Using MS analysis and a phospho-state- and -site-specific antibody, we found that Ser-648, located in the FZD(6) C terminus, is efficiently phosphorylated by casein kinase 1 is an element of (CK1 is an element of) and that this phosphorylation requires the scaffolding protein Dishevelled (DVL). In an overexpression system, DVL1, -2, and -3 promoted CK1-mediated FZD(6) phosphorylation on Ser-648. This DVL activity required an intact DEP domain and FZD-mediated recruitment of this domain to the cell membrane....
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titleDishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.
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titleDishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization.
authorStrakova, Katerina ; Kowalski-Jahn, Maria ; Gybel, Tomas ; Valnohova, Jana ; Dhople, Vishnu M ; Harnos, Jakub ; Bernatik, Ondrej ; Ganji, Ranjani Sri ; Zdrahal, Zbynek ; Mulder, Jan ; Lindskog, Cecilia ; Bryja, Vitezslav ; Schulte, Gunnar
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