schliessen

Filtern

 

Bibliotheken

All three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.

Inositol 1,4,5-trisphosphate (IP) evokes Ca release through IP receptors (IPRs) to generate both local Ca puffs arising from concerted openings of clustered IPRs and cell-wide Ca waves. Imaging Ca puffs with single-channel resolution yields information on the localization and properties of native IP... Full description

Journal Title: Science signaling December 18, 2018, Vol.11(561)
Main Author: Lock, Jeffrey T
Other Authors: Alzayady, Kamil J , Yule, David I , Parker, Ian
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1937-9145 ; DOI: 10.1126/scisignal.aau0344
Link: http://search.proquest.com/docview/2158553853/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest2158553853
title: All three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.
format: Article
creator:
  • Lock, Jeffrey T
  • Alzayady, Kamil J
  • Yule, David I
  • Parker, Ian
subjects:
  • Crispr-Cas Systems–Metabolism
  • Calcium–Metabolism
  • Calcium Signaling–Antagonists & Inhibitors
  • Hek293 Cells–Genetics
  • Humans–Metabolism
  • Inositol 1,4,5-Trisphosphate–Metabolism
  • Inositol 1,4,5-Trisphosphate Receptors–Metabolism
  • Kinetics–Metabolism
  • Protein Isoforms–Metabolism
  • Itpr3 Protein, Human
  • Inositol 1,4,5-Trisphosphate Receptors
  • Protein Isoforms
  • Inositol 1,4,5-Trisphosphate
  • Calcium
ispartof: Science signaling, December 18, 2018, Vol.11(561)
description: Inositol 1,4,5-trisphosphate (IP) evokes Ca release through IP receptors (IPRs) to generate both local Ca puffs arising from concerted openings of clustered IPRs and cell-wide Ca waves. Imaging Ca puffs with single-channel resolution yields information on the localization and properties of native IPRs in intact cells, but interpretation has been complicated because cells express varying proportions of three structurally and functionally distinct isoforms of IPRs. Here, we used TIRF and light-sheet microscopy to image Ca puffs in HEK-293 cell lines generated by CRISPR-Cas9 technology to express exclusively IPR type 1, 2, or 3. Photorelease of the IP analog i-IP in all three cell lines evoked puffs with largely similar mean amplitudes, temporal characteristics, and spatial extents. Moreover, the single-channel Ca flux was similar among isoforms, indicating that clusters of different IPR isoforms contain comparable numbers of active channels. Our results show that all three IPR isoforms cluster...
language: eng
source:
identifier: E-ISSN: 1937-9145 ; DOI: 10.1126/scisignal.aau0344
fulltext: fulltext
issn:
  • 19379145
  • 1937-9145
url: Link


@attributes
ID350205123
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid2158553853
sourceidproquest
recordidTN_proquest2158553853
sourcesystemPC
pqid2158553853
display
typearticle
titleAll three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.
creatorLock, Jeffrey T ; Alzayady, Kamil J ; Yule, David I ; Parker, Ian
contributorLock, Jeffrey T (correspondence author) ; Lock, Jeffrey T (record owner)
ispartofScience signaling, December 18, 2018, Vol.11(561)
identifierE-ISSN: 1937-9145 ; DOI: 10.1126/scisignal.aau0344
subjectCrispr-Cas Systems–Metabolism ; Calcium–Metabolism ; Calcium Signaling–Antagonists & Inhibitors ; Hek293 Cells–Genetics ; Humans–Metabolism ; Inositol 1,4,5-Trisphosphate–Metabolism ; Inositol 1,4,5-Trisphosphate Receptors–Metabolism ; Kinetics–Metabolism ; Protein Isoforms–Metabolism ; Itpr3 Protein, Human ; Inositol 1,4,5-Trisphosphate Receptors ; Protein Isoforms ; Inositol 1,4,5-Trisphosphate ; Calcium
languageeng
source
descriptionInositol 1,4,5-trisphosphate (IP) evokes Ca release through IP receptors (IPRs) to generate both local Ca puffs arising from concerted openings of clustered IPRs and cell-wide Ca waves. Imaging Ca puffs with single-channel resolution yields information on the localization and properties of native IPRs in intact cells, but interpretation has been complicated because cells express varying proportions of three structurally and functionally distinct isoforms of IPRs. Here, we used TIRF and light-sheet microscopy to image Ca puffs in HEK-293 cell lines generated by CRISPR-Cas9 technology to express exclusively IPR type 1, 2, or 3. Photorelease of the IP analog i-IP in all three cell lines evoked puffs with largely similar mean amplitudes, temporal characteristics, and spatial extents. Moreover, the single-channel Ca flux was similar among isoforms, indicating that clusters of different IPR isoforms contain comparable numbers of active channels. Our results show that all three IPR isoforms cluster...
version4
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/2158553853/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Lock, Jeffrey T
1Alzayady, Kamil J
2Yule, David I
3Parker, Ian
titleAll three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.
subject
0Crispr-Cas Systems–Metabolism
1Calcium–Metabolism
2Calcium Signaling–Antagonists & Inhibitors
3Hek293 Cells–Genetics
4Humans–Metabolism
5Inositol 1,4,5-Trisphosphate–Metabolism
6Inositol 1,4,5-Trisphosphate Receptors–Metabolism
7Kinetics–Metabolism
8Protein Isoforms–Metabolism
9Itpr3 Protein, Human
10Inositol 1,4,5-Trisphosphate Receptors
11Protein Isoforms
12Inositol 1,4,5-Trisphosphate
13Calcium
general
0English
110.1126/scisignal.aau0344
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest2158553853
issn
019379145
11937-9145
rsrctypearticle
creationdate2018
addtitleScience signaling
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorLock, Jeffrey T
startdate20181218
enddate20181218
citationvol 11 issue 561
lsr30VSR-Enriched:[description, pqid, issn]
sort
titleAll three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.
authorLock, Jeffrey T ; Alzayady, Kamil J ; Yule, David I ; Parker, Ian
creationdate20181218
lso0120181218
facets
frbrgroupid6842601932544730868
frbrtype5
newrecords20181225
languageeng
creationdate2018
topic
0Crispr-Cas Systems–Metabolism
1Calcium–Metabolism
2Calcium Signaling–Antagonists & Inhibitors
3Hek293 Cells–Genetics
4Humans–Metabolism
5Inositol 1,4,5-Trisphosphate–Metabolism
6Inositol 1,4,5-Trisphosphate Receptors–Metabolism
7Kinetics–Metabolism
8Protein Isoforms–Metabolism
9Itpr3 Protein, Human
10Inositol 1,4,5-Trisphosphate Receptors
11Protein Isoforms
12Inositol 1,4,5-Trisphosphate
13Calcium
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Lock, Jeffrey T
1Alzayady, Kamil J
2Yule, David I
3Parker, Ian
jtitleScience signaling
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Lock
1Alzayady
2Yule
3Parker
aufirst
0Jeffrey T
1Kamil J
2David I
3Ian
au
0Lock, Jeffrey T
1Alzayady, Kamil J
2Yule, David I
3Parker, Ian
addauLock, Jeffrey T
atitleAll three IP3 receptor isoforms generate Ca2+ puffs that display similar characteristics.
jtitleScience signaling
risdate20181218
volume11
issue561
eissn1937-9145
formatjournal
genrearticle
ristypeJOUR
doi10.1126/scisignal.aau0344
urlhttp://search.proquest.com/docview/2158553853/
issn19450877
date2018-12-18