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Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus

The aim of this study was to investigate the influence of cell cycle on transfection efficiency. Counterflow centrifugal elutriation was used which avoids possible side-effects from chemical treatment of cells. With this method, cell populations were fractionated by means of size and density, and fr... Full description

Journal Title: Gene Therapy Mar 2000, pp.401-7
Main Author: Brunner, S
Other Authors: Sauer, T , Carotta, S , Cotten, M , Saltik, M , Wagner, E
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
ID: ISSN: 09697128 ; E-ISSN: 14765462 ; DOI: 10.1038/sj.gt.3301102
Link: http://search.proquest.com/docview/218698443/?pq-origsite=primo
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recordid: proquest218698443
title: Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus
format: Article
creator:
  • Brunner, S
  • Sauer, T
  • Carotta, S
  • Cotten, M
  • Saltik, M
  • Wagner, E
subjects:
  • Adenoviridae–Genetics
  • Cation Exchange Resins–Administration & Dosage
  • Cell Cycle–Genetics
  • DNA–Administration & Dosage
  • DNA–Genetics
  • Drug Carriers–Genetics
  • Gene Transfer Techniques–Administration & Dosage
  • Genes, Reporter–Genetics
  • Genetic Vectors–Administration & Dosage
  • Genetic Vectors–Genetics
  • Humans–Administration & Dosage
  • K562 Cells–Chemistry
  • Lipids–Genetics
  • Liposomes–Administration & Dosage
  • Polyethyleneimine–Chemistry
  • Polyethyleneimine–Chemistry
  • Transfection–Chemistry
  • Transferrin–Chemistry
  • Transferrin–Chemistry
  • Cation Exchange Resins
  • Drug Carriers
  • Lipids
  • Lipofectamine
  • Liposomes
  • Transferrin
  • Polyethyleneimine
  • DNA
ispartof: Gene Therapy, Mar 2000, pp.401-7
description: The aim of this study was to investigate the influence of cell cycle on transfection efficiency. Counterflow centrifugal elutriation was used which avoids possible side-effects from chemical treatment of cells. With this method, cell populations were fractionated by means of size and density, and fractions...
language: eng
source:
identifier: ISSN: 09697128 ; E-ISSN: 14765462 ; DOI: 10.1038/sj.gt.3301102
fulltext: fulltext
issn:
  • 09697128
  • 0969-7128
  • 14765462
  • 1476-5462
url: Link


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descriptionThe aim of this study was to investigate the influence of cell cycle on transfection efficiency. Counterflow centrifugal elutriation was used which avoids possible side-effects from chemical treatment of cells. With this method, cell populations were fractionated by means of size and density, and fractions...
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titleCell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus
authorBrunner, S ; Sauer, T ; Carotta, S ; Cotten, M ; Saltik, M ; Wagner, E
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6Gene Transfer Techniques–Administration & Dosage
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abstractThe aim of this study was to investigate the influence of cell cycle on transfection efficiency. Counterflow centrifugal elutriation was used which avoids possible side-effects from chemical treatment of cells. With this method, cell populations were fractionated by means of size and density, and fractions...
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