schliessen

Filtern

 

Bibliotheken

Intracellular trafficking of nonviral vectors

Nonviral vectors continue to be attractive alternatives to viruses due to their low toxicity and immunogenicity, lack of pathogenicity, and ease of pharmacologic production. However, nonviral vectors also continue to suffer from relatively low levels of gene transfer compared to viruses, thus the dr... Full description

Journal Title: Gene Therapy Dec 2005, Vol.12(24), pp.1734-1751
Main Author: Medina-Kauwe, L
Other Authors: Xie, J , Hamm-Alvarez, S
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 09697128 ; E-ISSN: 14765462 ; DOI: 10.1038/sj.gt.3302592
Link: http://search.proquest.com/docview/218705358/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest218705358
title: Intracellular trafficking of nonviral vectors
format: Article
creator:
  • Medina-Kauwe, L
  • Xie, J
  • Hamm-Alvarez, S
subjects:
  • Endocytosis
  • Gene Transfer Techniques
  • Genetic Vectors
  • Genetic Therapy -- Methods
ispartof: Gene Therapy, Dec 2005, Vol.12(24), pp.1734-1751
description: Nonviral vectors continue to be attractive alternatives to viruses due to their low toxicity and immunogenicity, lack of pathogenicity, and ease of pharmacologic production. However, nonviral vectors also continue to suffer from relatively low levels of gene transfer compared to viruses, thus the drive to improve these vectors continues. Many studies on vector-cell interactions have reported that nonviral vectors bind and enter cells efficiently, but yield low gene expression, thus directing our attention to the intracellular trafficking of these vectors to understand where the obstacles occur. Here, we will review nonviral vector trafficking pathways, which will be considered here as the steps from cell binding to nuclear delivery. Studies on the intracellular trafficking of nonviral vectors has given us valuable insights into the barriers these vectors must overcome to mediate efficient gene transfer. Importantly, we will highlight the different approaches used by researchers to overcome certain trafficking barriers to gene transfer, many of which incorporate components from biological systems that have naturally evolved the capacity to overcome such obstacles. The tools used to study trafficking pathways will also be discussed.Gene Therapy (2005) 12, 1734-1751. doi:10.1038/sj.gt.3302592; published online 4 August 2005
language: eng
source:
identifier: ISSN: 09697128 ; E-ISSN: 14765462 ; DOI: 10.1038/sj.gt.3302592
fulltext: fulltext
issn:
  • 09697128
  • 0969-7128
  • 14765462
  • 1476-5462
url: Link


@attributes
ID380141132
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid218705358
sourceidproquest
recordidTN_proquest218705358
sourcesystemOther
pqid218705358
galeid182791093
display
typearticle
titleIntracellular trafficking of nonviral vectors
creatorMedina-Kauwe, L ; Xie, J ; Hamm-Alvarez, S
ispartofGene Therapy, Dec 2005, Vol.12(24), pp.1734-1751
identifier
descriptionNonviral vectors continue to be attractive alternatives to viruses due to their low toxicity and immunogenicity, lack of pathogenicity, and ease of pharmacologic production. However, nonviral vectors also continue to suffer from relatively low levels of gene transfer compared to viruses, thus the drive to improve these vectors continues. Many studies on vector-cell interactions have reported that nonviral vectors bind and enter cells efficiently, but yield low gene expression, thus directing our attention to the intracellular trafficking of these vectors to understand where the obstacles occur. Here, we will review nonviral vector trafficking pathways, which will be considered here as the steps from cell binding to nuclear delivery. Studies on the intracellular trafficking of nonviral vectors has given us valuable insights into the barriers these vectors must overcome to mediate efficient gene transfer. Importantly, we will highlight the different approaches used by researchers to overcome certain trafficking barriers to gene transfer, many of which incorporate components from biological systems that have naturally evolved the capacity to overcome such obstacles. The tools used to study trafficking pathways will also be discussed.Gene Therapy (2005) 12, 1734-1751. doi:10.1038/sj.gt.3302592; published online 4 August 2005
languageeng
source
subjectEndocytosis ; Gene Transfer Techniques ; Genetic Vectors ; Genetic Therapy -- Methods;
version8
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/218705358/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Medina-Kauwe, L
1Xie, J
2Hamm-Alvarez, S
titleIntracellular trafficking of nonviral vectors
descriptionNonviral vectors continue to be attractive alternatives to viruses due to their low toxicity and immunogenicity, lack of pathogenicity, and ease of pharmacologic production. However, nonviral vectors also continue to suffer from relatively low levels of gene transfer compared to viruses, thus the drive to improve these vectors continues. Many studies on vector-cell interactions have reported that nonviral vectors bind and enter cells efficiently, but yield low gene expression, thus directing our attention to the intracellular trafficking of these vectors to understand where the obstacles occur. Here, we will review nonviral vector trafficking pathways, which will be considered here as the steps from cell binding to nuclear delivery. Studies on the intracellular trafficking of nonviral vectors has given us valuable insights into the barriers these vectors must overcome to mediate efficient gene transfer. Importantly, we will highlight the different approaches used by researchers to overcome certain trafficking barriers to gene transfer, many of which incorporate components from biological systems that have naturally evolved the capacity to overcome such obstacles. The tools used to study trafficking pathways will also be discussed.Gene Therapy (2005) 12, 1734-1751. doi:10.1038/sj.gt.3302592; published online 4 August 2005
general
0English
1Nature Publishing Group
210.1038/sj.gt.3302592
3Medical Database
4Health & Medical Collection (Alumni edition)
5Medical Database (Alumni edition)
6ProQuest Pharma Collection
7Health & Medical Collection
8Neurosciences Abstracts
9ProQuest Public Health
10Biological Science Database
11Research Library China
12Engineering Research Database
13Technology Research Database
14ProQuest Research Library
15ProQuest Biological Science Collection
16ProQuest Central
17ProQuest Engineering Collection
18ProQuest Hospital Collection
19ProQuest Natural Science Collection
20ProQuest Technology Collection
21Research Library (Alumni edition)
22Hospital Premium Collection (Alumni edition)
23ProQuest SciTech Collection
24ProQuest Health & Medical Complete
25ProQuest Medical Library
26Natural Science Collection
27ProQuest Central (new)
28ProQuest Central K-12
29ProQuest Central Korea
30Research Library Prep
31SciTech Premium Collection
32Health Research Premium Collection
33Health Research Premium Collection (Alumni edition)
34ProQuest Central Essentials
35ProQuest Central China
sourceidproquest
recordidproquest218705358
issn
009697128
10969-7128
214765462
31476-5462
rsrctypearticle
creationdate2005
addtitleGene Therapy
searchscope
01000273
11006759
21006761
31006762
41006815
51006993
61007067
71007403
81007444
91007490
101007536
111007538
121007617
131007856
141007902
151007945
161008008
171009127
181009168
191009384
2010000002
2110000004
2210000005
2310000013
2410000015
2510000022
2610000025
2710000034
2810000038
2910000039
3010000041
3110000047
3210000050
3310000053
3410000064
3510000118
3610000119
3710000120
3810000155
3910000156
4010000157
4110000158
4210000164
4310000198
4410000209
4510000217
4610000238
4710000253
4810000255
4910000256
5010000257
5110000258
5210000259
5310000260
5410000268
5510000270
5610000271
5710000281
5810000300
5910000302
60proquest
scope
01000273
11006759
21006761
31006762
41006815
51006993
61007067
71007403
81007444
91007490
101007536
111007538
121007617
131007856
141007902
151007945
161008008
171009127
181009168
191009384
2010000002
2110000004
2210000005
2310000013
2410000015
2510000022
2610000025
2710000034
2810000038
2910000039
3010000041
3110000047
3210000050
3310000053
3410000064
3510000118
3610000119
3710000120
3810000155
3910000156
4010000157
4110000158
4210000164
4310000198
4410000209
4510000217
4610000238
4710000253
4810000255
4910000256
5010000257
5110000258
5210000259
5310000260
5410000268
5510000270
5610000271
5710000281
5810000300
5910000302
60proquest
lsr43
01000273true
11006759true
21006761true
31006762true
41006815true
51006993true
61007067true
71007403false
81007444false
91007490false
101007536false
111007538false
121007617true
131007856true
141007902true
151007945true
161008008true
171009127true
181009168true
191009384true
2010000002false
2110000004false
2210000005false
2310000013false
2410000015false
2510000022false
2610000025true
2710000034true
2810000038true
2910000039true
3010000041false
3110000047true
3210000050true
3310000053false
3410000064true
3510000118true
3610000119true
3710000120true
3810000155true
3910000156true
4010000157true
4110000158true
4210000164true
4310000198false
4410000209false
4510000217false
4610000238true
4710000253true
4810000255true
4910000256true
5010000257true
5110000258true
5210000259true
5310000260true
5410000268true
5510000270true
5610000271true
5710000281true
5810000300true
5910000302true
startdate20051201
enddate20051201
citationpf 1734 pt 1751 vol 12 issue 24
lsr30VSR-Enriched:[pqid, subject, galeid]
sort
titleIntracellular trafficking of nonviral vectors
authorMedina-Kauwe, L ; Xie, J ; Hamm-Alvarez, S
creationdate20051201
lso0120051201
facets
frbrgroupid-2307085701631589567
frbrtype5
languageeng
creationdate2005
collection
0Medical Database
1Health & Medical Collection (Alumni edition)
2Medical Database (Alumni edition)
3ProQuest Pharma Collection
4Health & Medical Collection
5Neurosciences Abstracts
6ProQuest Public Health
7Biological Science Database
8Research Library China
9Engineering Research Database
10Technology Research Database
11ProQuest Research Library
12ProQuest Biological Science Collection
13ProQuest Central
14ProQuest Engineering Collection
15ProQuest Hospital Collection
16ProQuest Natural Science Collection
17ProQuest Technology Collection
18Research Library (Alumni edition)
19Hospital Premium Collection (Alumni edition)
20ProQuest SciTech Collection
21ProQuest Health & Medical Complete
22ProQuest Medical Library
23Natural Science Collection
24ProQuest Central (new)
25ProQuest Central K-12
26ProQuest Central Korea
27Research Library Prep
28SciTech Premium Collection
29Health Research Premium Collection
30Health Research Premium Collection (Alumni edition)
31ProQuest Central Essentials
32ProQuest Central China
prefilterarticles
rsrctypearticles
creatorcontrib
0Medina-Kauwe, L
1Xie, J
2Hamm-Alvarez, S
jtitleGene Therapy
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Medina-Kauwe
1Xie
2Hamm-Alvarez
aufirst
0L
1J
2S
au
0Medina-Kauwe, L
1Xie, J
2Hamm-Alvarez, S
atitleIntracellular trafficking of nonviral vectors
jtitleGene Therapy
risdate20051201
volume12
issue24
spage1734
epage1751
pages1734-1751
issn09697128
eissn14765462
formatjournal
genrearticle
ristypeJOUR
abstractNonviral vectors continue to be attractive alternatives to viruses due to their low toxicity and immunogenicity, lack of pathogenicity, and ease of pharmacologic production. However, nonviral vectors also continue to suffer from relatively low levels of gene transfer compared to viruses, thus the drive to improve these vectors continues. Many studies on vector-cell interactions have reported that nonviral vectors bind and enter cells efficiently, but yield low gene expression, thus directing our attention to the intracellular trafficking of these vectors to understand where the obstacles occur. Here, we will review nonviral vector trafficking pathways, which will be considered here as the steps from cell binding to nuclear delivery. Studies on the intracellular trafficking of nonviral vectors has given us valuable insights into the barriers these vectors must overcome to mediate efficient gene transfer. Importantly, we will highlight the different approaches used by researchers to overcome certain trafficking barriers to gene transfer, many of which incorporate components from biological systems that have naturally evolved the capacity to overcome such obstacles. The tools used to study trafficking pathways will also be discussed.Gene Therapy (2005) 12, 1734-1751. doi:10.1038/sj.gt.3302592; published online 4 August 2005
copHoundmills
pubNature Publishing Group
doi10.1038/sj.gt.3302592
urlhttp://search.proquest.com/docview/218705358/
date2005-12-01