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Genospheres: self-assembling nucleic acid-lipid nanoparticles suitable for targeted gene delivery

We describe the assembly of a cationic lipid-nucleic acid nanoparticle from a liquid monophase containing water and a water miscible organic solvent where both lipid and DNA components are separately soluble prior to their combination. Upon removal of the organic solvent, stable and homogenously siz... Full description

Journal Title: Gene Therapy Apr 2006, Vol.13(7), pp.646-51
Main Author: Hayes, M
Other Authors: Drummond, D , Kirpotin, D , Zheng, W , Noble, C , Park, J , Marks, J , Benz, C , Hong, K
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
DNA
ID: ISSN: 09697128 ; DOI: 10.1038/sj.gt.3302699
Link: http://search.proquest.com/docview/218708042/?pq-origsite=primo
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title: Genospheres: self-assembling nucleic acid-lipid nanoparticles suitable for targeted gene delivery
format: Article
creator:
  • Hayes, M
  • Drummond, D
  • Kirpotin, D
  • Zheng, W
  • Noble, C
  • Park, J
  • Marks, J
  • Benz, C
  • Hong, K
subjects:
  • DNA–Administration & Dosage
  • Drug Carriers–Instrumentation
  • Gene Targeting–Methods
  • Genetic Therapy–Genetics
  • Genetic Therapy–Methods
  • Humans–Immunology
  • Immunoglobulin Variable Region–Immunology
  • Liposomes–Immunology
  • Microscopy, Electron–Immunology
  • Nanostructures–Immunology
  • Nanotechnology–Immunology
  • Phosphatidylethanolamines–Immunology
  • Polyethylene Glycols–Immunology
  • Receptor, Erbb-2–Immunology
  • Nanoparticles
  • Genetics
  • Lipids
  • Deoxyribonucleic Acid
  • DNA
  • Drug Carriers
  • Immunoglobulin Variable Region
  • Liposomes
  • Phosphatidylethanolamines
  • Polyethylene Glycols
  • Polyethylene Glycol-Distearoylphosphatidylethanolamine
  • DNA
  • Receptor, Erbb-2
ispartof: Gene Therapy, Apr 2006, Vol.13(7), pp.646-51
description: We describe the assembly of a cationic lipid-nucleic acid nanoparticle from a liquid monophase containing water and a water miscible organic solvent where both lipid and DNA components are separately soluble prior to their combination. Upon removal of the organic solvent, stable and homogenously sized (70-100 nm) lipid-nucleic acid nanoparticles (Genospheres) were formed. The low accessibility (100-fold) in HER2 overexpressing SK-BR-3 breast cancer cells was dependent on the degree of PEGylation, where the incorporation of high amounts of PEG-lipid on the particle surface (up to 5 mol%) had only a minor effect on the transfection activity of the targeted Genospheres. In summary, this work describes a novel, readily scalable method for preparing highly stable immunotargeted nucleic acid delivery vehicles capable of achieving a high degree of specific transfection activity.
language: eng
source:
identifier: ISSN: 09697128 ; DOI: 10.1038/sj.gt.3302699
fulltext: fulltext
issn:
  • 09697128
  • 0969-7128
url: Link


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titleGenospheres: self-assembling nucleic acid-lipid nanoparticles suitable for targeted gene delivery
creatorHayes, M ; Drummond, D ; Kirpotin, D ; Zheng, W ; Noble, C ; Park, J ; Marks, J ; Benz, C ; Hong, K
ispartofGene Therapy, Apr 2006, Vol.13(7), pp.646-51
identifierISSN: 09697128 ; DOI: 10.1038/sj.gt.3302699
subjectDNA–Administration & Dosage ; Drug Carriers–Instrumentation ; Gene Targeting–Methods ; Genetic Therapy–Genetics ; Genetic Therapy–Methods ; Humans–Immunology ; Immunoglobulin Variable Region–Immunology ; Liposomes–Immunology ; Microscopy, Electron–Immunology ; Nanostructures–Immunology ; Nanotechnology–Immunology ; Phosphatidylethanolamines–Immunology ; Polyethylene Glycols–Immunology ; Receptor, Erbb-2–Immunology ; Nanoparticles ; Genetics ; Lipids ; Deoxyribonucleic Acid ; DNA ; Drug Carriers ; Immunoglobulin Variable Region ; Liposomes ; Phosphatidylethanolamines ; Polyethylene Glycols ; Polyethylene Glycol-Distearoylphosphatidylethanolamine ; DNA ; Receptor, Erbb-2
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titleGenospheres: self-assembling nucleic acid-lipid nanoparticles suitable for targeted gene delivery
authorHayes, M ; Drummond, D ; Kirpotin, D ; Zheng, W ; Noble, C ; Park, J ; Marks, J ; Benz, C ; Hong, K
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6Immunoglobulin Variable Region–Immunology
7Liposomes–Immunology
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abstractWe describe the assembly of a cationic lipid-nucleic acid nanoparticle from a liquid monophase containing water and a water miscible organic solvent where both lipid and DNA components are separately soluble prior to their combination. Upon removal of the organic solvent, stable and homogenously sized (70-100 nm) lipid-nucleic acid nanoparticles (Genospheres) were formed. The low accessibility (100-fold) in HER2 overexpressing SK-BR-3 breast cancer cells was dependent on the degree of PEGylation, where the incorporation of high amounts of PEG-lipid on the particle surface (up to 5 mol%) had only a minor effect on the transfection activity of the targeted Genospheres. In summary, this work describes a novel, readily scalable method for preparing highly stable immunotargeted nucleic acid delivery vehicles capable of achieving a high degree of specific transfection activity.
copHoundmills
pubNature Publishing Group
doi10.1038/sj.gt.3302699
urlhttp://search.proquest.com/docview/218708042/
eissn14765462
date2006-04-01