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Amelioration of established collagen induced arthritis by systemic IL-10 gene delivery

A novel formulation of cationic liposomes containing the novel cytofectin ACHx was used for delivery of an anti-inflammatory cytokine gene, IL-10, to mice with established collagen induced arthritis. A single intraperitoneal injection of human IL-10 expression plasmid complexed with liposomes 2 to 4... Full description

Journal Title: Gene Therapy Jun 2000, Vol.7(11), pp.967-77
Main Author: Fellowes, R
Other Authors: Etheridge, C , Coade, S , Cooper, R , Stewart, L , Miller, A , Woo, P
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 09697128 ; DOI: 10.1038/sj.gt.3301165
Link: http://search.proquest.com/docview/218727125/?pq-origsite=primo
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title: Amelioration of established collagen induced arthritis by systemic IL-10 gene delivery
format: Article
creator:
  • Fellowes, R
  • Etheridge, C
  • Coade, S
  • Cooper, R
  • Stewart, L
  • Miller, A
  • Woo, P
subjects:
  • Animals–Therapy
  • Arthritis, Experimental–Methods
  • Cations–Administration & Dosage
  • Collagen–Analysis
  • Dose-Response Relationship, Drug–Genetics
  • Gene Expression–Administration & Dosage
  • Genetic Therapy–Immunology
  • Genetic Vectors–Analysis
  • Humans–Methods
  • Immunohistochemistry–Methods
  • In Situ Hybridization–Methods
  • Injections, Intraperitoneal–Methods
  • Interleukin-10–Methods
  • Interleukin-10–Methods
  • Liposomes–Methods
  • Macrophages–Methods
  • Mice–Methods
  • Mice, Inbred Dba–Methods
  • RNA, Messenger–Methods
  • Reverse Transcriptase Polymerase Chain Reaction–Methods
  • Statistics, Nonparametric–Methods
  • Transfection–Methods
  • Cations
  • Liposomes
  • RNA, Messenger
  • Interleukin-10
  • Collagen
ispartof: Gene Therapy, Jun 2000, Vol.7(11), pp.967-77
description: A novel formulation of cationic liposomes containing the novel cytofectin ACHx was used for delivery of an anti-inflammatory cytokine gene, IL-10, to mice with established collagen induced arthritis. A single intraperitoneal injection of human IL-10 expression plasmid complexed with liposomes 2 to 4 days after the onset of arthritis was sufficient to give significant and prolonged amelioration of arthritis for 30 days. Preliminary experiments suggested that the therapeutic effect was IL-10 dose-dependent. The distribution of the human IL-10 DNA after injection was widespread, including the inflamed paws. Human IL-10 mRNA was also detected in the paws 24 h after injection. IL-10 protein was below the level of detection in paws and serum but was detected in some tissues up to 10 days after injection. The target cell of transfection was demonstrated to be the macrophage. These results suggest that systemic therapy with plasmid DNA complexed with cationic liposomes merits further development as an alternative method for anti-inflammatory treatment of arthritis.
language: eng
source:
identifier: ISSN: 09697128 ; DOI: 10.1038/sj.gt.3301165
fulltext: fulltext
issn:
  • 09697128
  • 0969-7128
url: Link


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titleAmelioration of established collagen induced arthritis by systemic IL-10 gene delivery
creatorFellowes, R ; Etheridge, C ; Coade, S ; Cooper, R ; Stewart, L ; Miller, A ; Woo, P
ispartofGene Therapy, Jun 2000, Vol.7(11), pp.967-77
identifierISSN: 09697128 ; DOI: 10.1038/sj.gt.3301165
subjectAnimals–Therapy ; Arthritis, Experimental–Methods ; Cations–Administration & Dosage ; Collagen–Analysis ; Dose-Response Relationship, Drug–Genetics ; Gene Expression–Administration & Dosage ; Genetic Therapy–Immunology ; Genetic Vectors–Analysis ; Humans–Methods ; Immunohistochemistry–Methods ; In Situ Hybridization–Methods ; Injections, Intraperitoneal–Methods ; Interleukin-10–Methods ; Interleukin-10–Methods ; Liposomes–Methods ; Macrophages–Methods ; Mice–Methods ; Mice, Inbred Dba–Methods ; RNA, Messenger–Methods ; Reverse Transcriptase Polymerase Chain Reaction–Methods ; Statistics, Nonparametric–Methods ; Transfection–Methods ; Cations ; Liposomes ; RNA, Messenger ; Interleukin-10 ; Collagen
descriptionA novel formulation of cationic liposomes containing the novel cytofectin ACHx was used for delivery of an anti-inflammatory cytokine gene, IL-10, to mice with established collagen induced arthritis. A single intraperitoneal injection of human IL-10 expression plasmid complexed with liposomes 2 to 4 days after the onset of arthritis was sufficient to give significant and prolonged amelioration of arthritis for 30 days. Preliminary experiments suggested that the therapeutic effect was IL-10 dose-dependent. The distribution of the human IL-10 DNA after injection was widespread, including the inflamed paws. Human IL-10 mRNA was also detected in the paws 24 h after injection. IL-10 protein was below the level of detection in paws and serum but was detected in some tissues up to 10 days after injection. The target cell of transfection was demonstrated to be the macrophage. These results suggest that systemic therapy with plasmid DNA complexed with cationic liposomes merits further development as an alternative method for anti-inflammatory treatment of arthritis.
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titleAmelioration of established collagen induced arthritis by systemic IL-10 gene delivery
descriptionA novel formulation of cationic liposomes containing the novel cytofectin ACHx was used for delivery of an anti-inflammatory cytokine gene, IL-10, to mice with established collagen induced arthritis. A single intraperitoneal injection of human IL-10 expression plasmid complexed with liposomes 2 to 4 days after the onset of arthritis was sufficient to give significant and prolonged amelioration of arthritis for 30 days. Preliminary experiments suggested that the therapeutic effect was IL-10 dose-dependent. The distribution of the human IL-10 DNA after injection was widespread, including the inflamed paws. Human IL-10 mRNA was also detected in the paws 24 h after injection. IL-10 protein was below the level of detection in paws and serum but was detected in some tissues up to 10 days after injection. The target cell of transfection was demonstrated to be the macrophage. These results suggest that systemic therapy with plasmid DNA complexed with cationic liposomes merits further development as an alternative method for anti-inflammatory treatment of arthritis.
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titleAmelioration of established collagen induced arthritis by systemic IL-10 gene delivery
authorFellowes, R ; Etheridge, C ; Coade, S ; Cooper, R ; Stewart, L ; Miller, A ; Woo, P
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5Gene Expression–Administration & Dosage
6Genetic Therapy–Immunology
7Genetic Vectors–Analysis
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abstractA novel formulation of cationic liposomes containing the novel cytofectin ACHx was used for delivery of an anti-inflammatory cytokine gene, IL-10, to mice with established collagen induced arthritis. A single intraperitoneal injection of human IL-10 expression plasmid complexed with liposomes 2 to 4 days after the onset of arthritis was sufficient to give significant and prolonged amelioration of arthritis for 30 days. Preliminary experiments suggested that the therapeutic effect was IL-10 dose-dependent. The distribution of the human IL-10 DNA after injection was widespread, including the inflamed paws. Human IL-10 mRNA was also detected in the paws 24 h after injection. IL-10 protein was below the level of detection in paws and serum but was detected in some tissues up to 10 days after injection. The target cell of transfection was demonstrated to be the macrophage. These results suggest that systemic therapy with plasmid DNA complexed with cationic liposomes merits further development as an alternative method for anti-inflammatory treatment of arthritis.
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pubNature Publishing Group
doi10.1038/sj.gt.3301165
urlhttp://search.proquest.com/docview/218727125/
eissn14765462
date2000-06-01