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Ultrasensitive Electrochemical Biosensor Developed by Probe Lengthening for Detection of Genomic DNA in Human Serum.

As an alternative to most of the reported nucleic acid amplification-based electrochemical DNA biosensors used for detection of trace levels of genomic DNA, we herein present a novel detection concept. The proposed system involves the conversion of two short double-stranded DNAs (dsDNAs), labeled wi... Full description

Journal Title: Analytical chemistry April 2, 2019, Vol.91(7), pp.4552-4558
Main Author: Chen, Jin-Yuan
Other Authors: Liu, Zhou-Jie , Wang, Xue-Wen , Ye, Chen-Liu , Zheng, Yan-Jie , Peng, Hua-Ping , Zhong, Guang-Xian , Liu, Ai-Lin , Chen, Wei , Lin, Xin-Hua
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.8b05692
Link: http://search.proquest.com/docview/2188586650/?pq-origsite=primo
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title: Ultrasensitive Electrochemical Biosensor Developed by Probe Lengthening for Detection of Genomic DNA in Human Serum.
format: Article
creator:
  • Chen, Jin-Yuan
  • Liu, Zhou-Jie
  • Wang, Xue-Wen
  • Ye, Chen-Liu
  • Zheng, Yan-Jie
  • Peng, Hua-Ping
  • Zhong, Guang-Xian
  • Liu, Ai-Lin
  • Chen, Wei
  • Lin, Xin-Hua
subjects:
  • Ligase Chain Reaction
  • Gold
  • Viruses
  • Biotin
  • Serum Albumin
  • Immobilization
  • Depletion
  • Target Detection
  • Biosensors
  • Biotin
  • Bovine Serum Albumin
  • Viruses
  • Deoxyribonucleic Acid–DNA
  • Electrochemistry
  • Deoxyribonucleic Acid–DNA
  • Diagnostic Systems
  • Acids
  • Biosensors
ispartof: Analytical chemistry, April 2, 2019, Vol.91(7), pp.4552-4558
description: As an alternative to most of the reported nucleic acid amplification-based electrochemical DNA biosensors used for detection of trace levels of genomic DNA, we herein present a novel detection concept. The proposed system involves the conversion of two short double-stranded DNAs (dsDNAs), labeled with a thiol-tag or biotin-tag, into a single integrated dsDNA containing thiol and biotin at both terminals in the presence of target DNA through ligase chain reaction (LCR) and followed by the immobilization of these integrated dsDNAs on a bovine serum albumin (BSA)-modified gold electrode surface. Owing to rapid depletion of the two short dsDNAs via LCR, the integrated dsDNAs were generated in an exponential manner so that this sensoring approach offered a limit of detection of 25 yoctomoles (15 copies in 50 μL sample volumes), a high discrimination of single-base mismatch and a wide linear concentration range (across 6 orders of magnitude) for target DNA. Significantly, the proposed sensor,...
language: eng
source:
identifier: E-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.8b05692
fulltext: fulltext
issn:
  • 15206882
  • 1520-6882
url: Link


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titleUltrasensitive Electrochemical Biosensor Developed by Probe Lengthening for Detection of Genomic DNA in Human Serum.
creatorChen, Jin-Yuan ; Liu, Zhou-Jie ; Wang, Xue-Wen ; Ye, Chen-Liu ; Zheng, Yan-Jie ; Peng, Hua-Ping ; Zhong, Guang-Xian ; Liu, Ai-Lin ; Chen, Wei ; Lin, Xin-Hua
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ispartofAnalytical chemistry, April 2, 2019, Vol.91(7), pp.4552-4558
identifierE-ISSN: 1520-6882 ; DOI: 10.1021/acs.analchem.8b05692
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subjectLigase Chain Reaction ; Gold ; Viruses ; Biotin ; Serum Albumin ; Immobilization ; Depletion ; Target Detection ; Biosensors ; Biotin ; Bovine Serum Albumin ; Viruses ; Deoxyribonucleic Acid–DNA ; Electrochemistry ; Deoxyribonucleic Acid–DNA ; Diagnostic Systems ; Acids ; Biosensors;
descriptionAs an alternative to most of the reported nucleic acid amplification-based electrochemical DNA biosensors used for detection of trace levels of genomic DNA, we herein present a novel detection concept. The proposed system involves the conversion of two short double-stranded DNAs (dsDNAs), labeled with a thiol-tag or biotin-tag, into a single integrated dsDNA containing thiol and biotin at both terminals in the presence of target DNA through ligase chain reaction (LCR) and followed by the immobilization of these integrated dsDNAs on a bovine serum albumin (BSA)-modified gold electrode surface. Owing to rapid depletion of the two short dsDNAs via LCR, the integrated dsDNAs were generated in an exponential manner so that this sensoring approach offered a limit of detection of 25 yoctomoles (15 copies in 50 μL sample volumes), a high discrimination of single-base mismatch and a wide linear concentration range (across 6 orders of magnitude) for target DNA. Significantly, the proposed sensor,...
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