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Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis

Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be in... Full description

Journal Title: International Journal of Molecular Sciences Feb 2019, Vol.20(12)
Main Author: Chen, Jiahui
Other Authors: Ren, Xueyi , Li, Limin , Lu, Shiyi , Chen, Tian , Tan, Liangtian , Liu, Manqing , Luo, Qingbin , Liang, Shaodong , Nie, Qinghua , Zhang, Xiquan , Luo, Wen
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 16616596 ; E-ISSN: 14220067 ; DOI: 10.3390/ijms20122923
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title: Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
format: Article
creator:
  • Chen, Jiahui
  • Ren, Xueyi
  • Li, Limin
  • Lu, Shiyi
  • Chen, Tian
  • Tan, Liangtian
  • Liu, Manqing
  • Luo, Qingbin
  • Liang, Shaodong
  • Nie, Qinghua
  • Zhang, Xiquan
  • Luo, Wen
subjects:
  • China
  • Hypertrophy
  • Pathways
  • Steroids
  • Hepatoma
  • Deposition
  • High Fat Diet
  • Genes
  • Nutrition Research
  • Adipocytes
  • Peroxisome Proliferator-Activated Receptors
  • Metabolism
  • Abdomen
  • Genomics
  • Lipids
  • Localization
  • Extracellular Matrix
  • Oils & Fats
  • Body Weight
  • Growth Factors
  • Growth Hormones
  • Adipogenesis
  • Proteins
  • Preadipocytes
  • Gene Expression
  • Insulin
  • Gene Sequencing
  • Gene Expression
  • Liver
  • Hepatocytes
  • Body Weight
  • Biosynthesis
  • Gene Expression
  • Fat Metabolism
  • Lipids
  • Metabolism
  • Liver
  • Cell Growth
  • Insulin
  • Insulin-Like Growth Factors
  • Poultry
  • Chickens
  • Insulin-Like Growth Factor II
  • Diet
  • China Agricultural University
  • Chinese Native Broiler
  • Abdominal Fat Deposition
  • Adipogenesis
  • Igf2bp1
  • High-Fat Diet
  • Differentially Expressed Gene
ispartof: International Journal of Molecular Sciences, Feb 2019, Vol.20(12)
description: Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.
language: eng
source:
identifier: ISSN: 16616596 ; E-ISSN: 14220067 ; DOI: 10.3390/ijms20122923
fulltext: fulltext_linktorsrc
issn:
  • 16616596
  • 1661-6596
  • 14220067
  • 1422-0067
url: Link


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titleIntegrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
creatorChen, Jiahui ; Ren, Xueyi ; Li, Limin ; Lu, Shiyi ; Chen, Tian ; Tan, Liangtian ; Liu, Manqing ; Luo, Qingbin ; Liang, Shaodong ; Nie, Qinghua ; Zhang, Xiquan ; Luo, Wen
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subjectChina ; Hypertrophy ; Pathways ; Steroids ; Hepatoma ; Deposition ; High Fat Diet ; Genes ; Nutrition Research ; Adipocytes ; Peroxisome Proliferator-Activated Receptors ; Metabolism ; Abdomen ; Genomics ; Lipids ; Localization ; Extracellular Matrix ; Oils & Fats ; Body Weight ; Growth Factors ; Growth Hormones ; Adipogenesis ; Proteins ; Preadipocytes ; Gene Expression ; Insulin ; Gene Sequencing ; Gene Expression ; Liver ; Hepatocytes ; Body Weight ; Biosynthesis ; Gene Expression ; Fat Metabolism ; Lipids ; Metabolism ; Liver ; Cell Growth ; Insulin ; Insulin-Like Growth Factors ; Poultry ; Chickens ; Insulin-Like Growth Factor II ; Diet ; China Agricultural University ; Chinese Native Broiler ; Abdominal Fat Deposition ; Adipogenesis ; Igf2bp1 ; High-Fat Diet ; Differentially Expressed Gene
descriptionExcessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.
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titleIntegrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
descriptionExcessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.
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titleIntegrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
authorChen, Jiahui ; Ren, Xueyi ; Li, Limin ; Lu, Shiyi ; Chen, Tian ; Tan, Liangtian ; Liu, Manqing ; Luo, Qingbin ; Liang, Shaodong ; Nie, Qinghua ; Zhang, Xiquan ; Luo, Wen
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abstractExcessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.
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