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Modulation of Shigella virulence in response to available oxygen in vivo

Bacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion s... Full description

Journal Title: Nature May 20, 2010, Vol.465(7296), pp.355-8
Main Author: Marteyn, Benoit
Other Authors: West, Nicholas , Browning, Douglas , Cole, Jeffery , Shaw, Jonathan , Palm, Fredrik , Mounier, Joelle , Prévost, Marie-Christine , Sansonetti, Philippe , Tang, Christoph
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 00280836 ; E-ISSN: 14764687
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title: Modulation of Shigella virulence in response to available oxygen in vivo
format: Article
creator:
  • Marteyn, Benoit
  • West, Nicholas
  • Browning, Douglas
  • Cole, Jeffery
  • Shaw, Jonathan
  • Palm, Fredrik
  • Mounier, Joelle
  • Prévost, Marie-Christine
  • Sansonetti, Philippe
  • Tang, Christoph
subjects:
  • Anaerobiosis–Drug Effects
  • Animals–Cytology
  • Cells, Cultured–Drug Effects
  • Epithelial Cells–Metabolism
  • Epithelial Cells–Microbiology
  • Epithelial Cells–Metabolism
  • Epithelial Cells–Cytology
  • Fumarates–Microbiology
  • Gastrointestinal Tract–Drug Effects
  • Gastrointestinal Tract–Physiology
  • Gene Expression Regulation, Bacterial–Metabolism
  • Hela Cells–Analysis
  • Host-Pathogen Interactions–Metabolism
  • Host-Pathogen Interactions–Pharmacology
  • Humans–Cytology
  • Mice–Metabolism
  • Nitrates–Pathogenicity
  • Oxidation-Reduction–Drug Effects
  • Oxygen–Genetics
  • Oxygen–Physiology
  • Oxygen–Physiology
  • Rabbits–Physiology
  • Shigella Flexneri–Physiology
  • Shigella Flexneri–Physiology
  • Shigella Flexneri–Physiology
  • Virulence–Physiology
  • Virulence–Physiology
  • Virulence–Physiology
  • Bacteria
  • Microbiology
  • Scanning Electron Microscopy
  • Binding Sites
  • Apoptosis
  • Fumarates
  • Nitrates
  • Fumaric Acid
  • Oxygen
ispartof: Nature, May 20, 2010, Vol.465(7296), pp.355-8
description: Bacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence. In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence. [PUBLICATION ]
language: eng
source:
identifier: ISSN: 00280836 ; E-ISSN: 14764687
fulltext: fulltext
issn:
  • 00280836
  • 0028-0836
  • 14764687
  • 1476-4687
url: Link


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titleModulation of Shigella virulence in response to available oxygen in vivo
creatorMarteyn, Benoit ; West, Nicholas ; Browning, Douglas ; Cole, Jeffery ; Shaw, Jonathan ; Palm, Fredrik ; Mounier, Joelle ; Prévost, Marie-Christine ; Sansonetti, Philippe ; Tang, Christoph
ispartofNature, May 20, 2010, Vol.465(7296), pp.355-8
identifierISSN: 00280836 ; E-ISSN: 14764687
subjectAnaerobiosis–Drug Effects ; Animals–Cytology ; Cells, Cultured–Drug Effects ; Epithelial Cells–Metabolism ; Epithelial Cells–Microbiology ; Epithelial Cells–Metabolism ; Epithelial Cells–Cytology ; Fumarates–Microbiology ; Gastrointestinal Tract–Drug Effects ; Gastrointestinal Tract–Physiology ; Gene Expression Regulation, Bacterial–Metabolism ; Hela Cells–Analysis ; Host-Pathogen Interactions–Metabolism ; Host-Pathogen Interactions–Pharmacology ; Humans–Cytology ; Mice–Metabolism ; Nitrates–Pathogenicity ; Oxidation-Reduction–Drug Effects ; Oxygen–Genetics ; Oxygen–Physiology ; Oxygen–Physiology ; Rabbits–Physiology ; Shigella Flexneri–Physiology ; Shigella Flexneri–Physiology ; Shigella Flexneri–Physiology ; Virulence–Physiology ; Virulence–Physiology ; Virulence–Physiology ; Bacteria ; Microbiology ; Scanning Electron Microscopy ; Binding Sites ; Apoptosis ; Fumarates ; Nitrates ; Fumaric Acid ; Oxygen
descriptionBacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence. In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence. [PUBLICATION ]
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titleModulation of Shigella virulence in response to available oxygen in vivo
descriptionBacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence. In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence. [PUBLICATION ]
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citationpf 355 pt 8 vol 465 issue 7296
lsr30VSR-Enriched:[doi, galeid, pages, pqid]
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titleModulation of Shigella virulence in response to available oxygen in vivo
authorMarteyn, Benoit ; West, Nicholas ; Browning, Douglas ; Cole, Jeffery ; Shaw, Jonathan ; Palm, Fredrik ; Mounier, Joelle ; Prévost, Marie-Christine ; Sansonetti, Philippe ; Tang, Christoph
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1Animals–Cytology
2Cells, Cultured–Drug Effects
3Epithelial Cells–Metabolism
4Epithelial Cells–Microbiology
5Epithelial Cells–Cytology
6Fumarates–Microbiology
7Gastrointestinal Tract–Drug Effects
8Gastrointestinal Tract–Physiology
9Gene Expression Regulation, Bacterial–Metabolism
10Hela Cells–Analysis
11Host-Pathogen Interactions–Metabolism
12Host-Pathogen Interactions–Pharmacology
13Humans–Cytology
14Mice–Metabolism
15Nitrates–Pathogenicity
16Oxidation-Reduction–Drug Effects
17Oxygen–Genetics
18Oxygen–Physiology
19Rabbits–Physiology
20Shigella Flexneri–Physiology
21Virulence–Physiology
22Bacteria
23Microbiology
24Scanning Electron Microscopy
25Binding Sites
26Apoptosis
27Fumarates
28Nitrates
29Fumaric Acid
30Oxygen
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abstractBacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence. In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence. [PUBLICATION ABSTRACT]
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urlhttp://search.proquest.com/docview/347842962/
doi10.1038/nature08970
date2010-05-20