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Enhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein

Aim: To explore cancer cell-specific phage fusion pVIII coat protein, identified using phage display, for targeted delivery of drug-loaded liposomes to MCF-7 breast cancer cells. Material & methods: An 8-mer landscape library f8/8 and a biopanning protocol against MCF-7 cells were used to select a l... Full description

Journal Title: Nanomedicine Jun 2010, Vol.5(4), pp.563-74
Main Author: Wang, Tao
Other Authors: D'Souza, Gerard Gm , Bedi, Deepa , Papahadjopoulos-Sternberg, Brigitte , Torchilin, Vladimir
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 1743-5889 ; E-ISSN: 1748-6963 ; DOI: 10.2217/nnm.10.30
Link: http://search.proquest.com/docview/365957236/?pq-origsite=primo
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title: Enhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein
format: Article
creator:
  • Wang, Tao
  • D'Souza, Gerard Gm
  • Bedi, Deepa
  • Papahadjopoulos-Sternberg, Brigitte
  • Torchilin, Vladimir
subjects:
  • Amino Acid Sequence–Administration & Dosage
  • Antineoplastic Agents–Chemistry
  • Bacteriophages–Chemistry
  • Blotting, Western–Administration & Dosage
  • Capsid Proteins–Pathology
  • Cell Line, Tumor–Pathology
  • Doxorubicin–Pathology
  • Drug Carriers–Pathology
  • Freeze Fracturing–Pathology
  • Humans–Pathology
  • Liposomes–Pathology
  • Molecular Sequence Data–Pathology
  • Neoplasms–Pathology
  • Antineoplastic Agents
  • Capsid Proteins
  • Drug Carriers
  • Liposomes
  • Doxorubicin
ispartof: Nanomedicine, Jun 2010, Vol.5(4), pp.563-74
description: Aim: To explore cancer cell-specific phage fusion pVIII coat protein, identified using phage display, for targeted delivery of drug-loaded liposomes to MCF-7 breast cancer cells. Material & methods: An 8-mer landscape library f8/8 and a biopanning protocol against MCF-7 cells were used to select a landscape phage protein bearing MCF-7-specific peptide. Size and morphology of doxorubicin-loaded liposomes modified with the tumor-specific phage fusion coat protein (phage-Doxil) were determined by dynamic light scattering and freeze-fraction electron microscopy. Topology of the phage protein in liposomes was examined by western blot. Association of phage-Doxil with MCF-7 cells was evaluated by fluorescence microscopy and fluorescence spectrometry. Selective targeting to MCF-7 was shown by FACS using a coculture model with target and nontarget cells. Phage-Doxil-induced tumor cell killing and apoptosis were confirmed by CellTiter-Blue® Assay and caspase-3/CPP32 fluorometric assay. Results: A...
language: eng
source:
identifier: ISSN: 1743-5889 ; E-ISSN: 1748-6963 ; DOI: 10.2217/nnm.10.30
fulltext: fulltext
issn:
  • 17435889
  • 1743-5889
  • 17486963
  • 1748-6963
url: Link


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titleEnhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein
creatorWang, Tao ; D'Souza, Gerard Gm ; Bedi, Deepa ; Papahadjopoulos-Sternberg, Brigitte ; Torchilin, Vladimir
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subjectAmino Acid Sequence–Administration & Dosage ; Antineoplastic Agents–Chemistry ; Bacteriophages–Chemistry ; Blotting, Western–Administration & Dosage ; Capsid Proteins–Pathology ; Cell Line, Tumor–Pathology ; Doxorubicin–Pathology ; Drug Carriers–Pathology ; Freeze Fracturing–Pathology ; Humans–Pathology ; Liposomes–Pathology ; Molecular Sequence Data–Pathology ; Neoplasms–Pathology ; Antineoplastic Agents ; Capsid Proteins ; Drug Carriers ; Liposomes ; Doxorubicin
descriptionAim: To explore cancer cell-specific phage fusion pVIII coat protein, identified using phage display, for targeted delivery of drug-loaded liposomes to MCF-7 breast cancer cells. Material & methods: An 8-mer landscape library f8/8 and a biopanning protocol against MCF-7 cells were used to select a landscape phage protein bearing MCF-7-specific peptide. Size and morphology of doxorubicin-loaded liposomes modified with the tumor-specific phage fusion coat protein (phage-Doxil) were determined by dynamic light scattering and freeze-fraction electron microscopy. Topology of the phage protein in liposomes was examined by western blot. Association of phage-Doxil with MCF-7 cells was evaluated by fluorescence microscopy and fluorescence spectrometry. Selective targeting to MCF-7 was shown by FACS using a coculture model with target and nontarget cells. Phage-Doxil-induced tumor cell killing and apoptosis were confirmed by CellTiter-Blue® Assay and caspase-3/CPP32 fluorometric assay. Results: A...
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titleEnhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein
descriptionAim: To explore cancer cell-specific phage fusion pVIII coat protein, identified using phage display, for targeted delivery of drug-loaded liposomes to MCF-7 breast cancer cells. Material & methods: An 8-mer landscape library f8/8 and a biopanning protocol against MCF-7 cells were used to select a landscape phage protein bearing MCF-7-specific peptide. Size and morphology of doxorubicin-loaded liposomes modified with the tumor-specific phage fusion coat protein (phage-Doxil) were determined by dynamic light scattering and freeze-fraction electron microscopy. Topology of the phage protein in liposomes was examined by western blot. Association of phage-Doxil with MCF-7 cells was evaluated by fluorescence microscopy and fluorescence spectrometry. Selective targeting to MCF-7 was shown by FACS using a coculture model with target and nontarget cells. Phage-Doxil-induced tumor cell killing and apoptosis were confirmed by CellTiter-Blue® Assay and caspase-3/CPP32 fluorometric assay. Results: A...
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12Neoplasms–Pathology
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titleEnhanced binding and killing of target tumor cells by drug-loaded liposomes modified with tumor-specific phage fusion coat protein
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abstractAim: To explore cancer cell-specific phage fusion pVIII coat protein, identified using phage display, for targeted delivery of drug-loaded liposomes to MCF-7 breast cancer cells. Material & methods: An 8-mer landscape library f8/8 and a biopanning protocol against MCF-7 cells were used to select a landscape phage protein bearing MCF-7-specific peptide. Size and morphology of doxorubicin-loaded liposomes modified with the tumor-specific phage fusion coat protein (phage-Doxil) were determined by dynamic light scattering and freeze-fraction electron microscopy. Topology of the phage protein in liposomes was examined by western blot. Association of phage-Doxil with MCF-7 cells was evaluated by fluorescence microscopy and fluorescence spectrometry. Selective targeting to MCF-7 was shown by FACS using a coculture model with target and nontarget cells. Phage-Doxil-induced tumor cell killing and apoptosis were confirmed by CellTiter-Blue® Assay and caspase-3/CPP32 fluorometric assay. Results: A...
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pubFuture Medicine Ltd
doi10.2217/nnm.10.30
urlhttp://search.proquest.com/docview/365957236/
date2010-06-01