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Synaptopodin regulates plasticity of dendritic spines in hippocampal neurons.

The spine apparatus is an essential component of dendritic spines of cortical and hippocampal neurons, yet its functions are still enigmatic. Synaptopodin (SP), an actin-binding protein, is tightly associated with the spine apparatus and it may play a role in synaptic plasticity, but it has not yet... Full description

Journal Title: The Journal of neuroscience : the official journal of the Society for Neuroscience January 28, 2009, Vol.29(4), pp.1017-1033
Main Author: Vlachos, Andreas
Other Authors: Korkotian, Eduard , Schonfeld, Eldi , Copanaki, Ekaterini , Deller, Thomas , Segal, Menahem
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: E-ISSN: 1529-2401 ; DOI: 10.1523/JNEUROSCI.5528-08.2009
Link: http://search.proquest.com/docview/66861825/?pq-origsite=primo
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title: Synaptopodin regulates plasticity of dendritic spines in hippocampal neurons.
format: Article
creator:
  • Vlachos, Andreas
  • Korkotian, Eduard
  • Schonfeld, Eldi
  • Copanaki, Ekaterini
  • Deller, Thomas
  • Segal, Menahem
subjects:
  • Acetaminophen–Pharmacology
  • Actins–Metabolism
  • Animals–Analogs & Derivatives
  • Animals, Newborn–Pharmacology
  • Antipyrine–Pharmacology
  • Caffeine–Metabolism
  • Calcium–Pharmacology
  • Cells, Cultured–Drug Effects
  • Codeine–Physiology
  • Dendritic Spines–Methods
  • Drug Combinations–Pharmacology
  • Electric Stimulation–Genetics
  • Excitatory Amino Acid Agents–Cytology
  • Green Fluorescent Proteins–Genetics
  • Hippocampus–Physiology
  • Microfilament Proteins–Drug Effects
  • Models, Biological–Physiology
  • Neuronal Plasticity–Cytology
  • Neurons
ispartof: The Journal of neuroscience : the official journal of the Society for Neuroscience, January 28, 2009, Vol.29(4), pp.1017-1033
description: The spine apparatus is an essential component of dendritic spines of cortical and hippocampal neurons, yet its functions are still enigmatic. Synaptopodin (SP), an actin-binding protein, is tightly associated with the spine apparatus and it may play a role in synaptic plasticity, but it has not yet been linked mechanistically to synaptic functions. We studied endogenous and transfected SP in dendritic spines of cultured hippocampal neurons and found that spines containing SP generate larger responses to flash photolysis of caged glutamate than SP-negative ones. An NMDA-receptor-mediated chemical long-term potentiation caused the accumulation of GFP-GluR1 in spine heads of control but not of shRNA-transfected, SP-deficient neurons. SP is linked to calcium stores, because their pharmacological blockade eliminated SP-related enhancement of glutamate responses, and release of calcium from stores produced an SP-dependent increase of GluR1 in spines. Thus, SP plays a crucial role in the calcium store-associated ability of neurons to undergo long-term plasticity.
language: eng
source:
identifier: E-ISSN: 1529-2401 ; DOI: 10.1523/JNEUROSCI.5528-08.2009
fulltext: fulltext
issn:
  • 15292401
  • 1529-2401
url: Link


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titleSynaptopodin regulates plasticity of dendritic spines in hippocampal neurons.
creatorVlachos, Andreas ; Korkotian, Eduard ; Schonfeld, Eldi ; Copanaki, Ekaterini ; Deller, Thomas ; Segal, Menahem
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subjectAcetaminophen–Pharmacology ; Actins–Metabolism ; Animals–Analogs & Derivatives ; Animals, Newborn–Pharmacology ; Antipyrine–Pharmacology ; Caffeine–Metabolism ; Calcium–Pharmacology ; Cells, Cultured–Drug Effects ; Codeine–Physiology ; Dendritic Spines–Methods ; Drug Combinations–Pharmacology ; Electric Stimulation–Genetics ; Excitatory Amino Acid Agents–Cytology ; Green Fluorescent Proteins–Genetics ; Hippocampus–Physiology ; Microfilament Proteins–Drug Effects ; Models, Biological–Physiology ; Neuronal Plasticity–Cytology ; Neurons
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descriptionThe spine apparatus is an essential component of dendritic spines of cortical and hippocampal neurons, yet its functions are still enigmatic. Synaptopodin (SP), an actin-binding protein, is tightly associated with the spine apparatus and it may play a role in synaptic plasticity, but it has not yet been linked mechanistically to synaptic functions. We studied endogenous and transfected SP in dendritic spines of cultured hippocampal neurons and found that spines containing SP generate larger responses to flash photolysis of caged glutamate than SP-negative ones. An NMDA-receptor-mediated chemical long-term potentiation caused the accumulation of GFP-GluR1 in spine heads of control but not of shRNA-transfected, SP-deficient neurons. SP is linked to calcium stores, because their pharmacological blockade eliminated SP-related enhancement of glutamate responses, and release of calcium from stores produced an SP-dependent increase of GluR1 in spines. Thus, SP plays a crucial role in the calcium store-associated ability of neurons to undergo long-term plasticity.
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