schliessen

Filtern

 

Bibliotheken

A novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.

Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Is... Full description

Journal Title: Cell transplantation 2009, Vol.18(4), pp.433-441
Main Author: Zou, Xiao Hui
Other Authors: Cai, Hong Xin , Yin, Zi , Chen, Xiao , Jiang, Yang Zi , Hu, Hu , Ouyang, Hong Wei
Format: Electronic Article Electronic Article
Language: English
Subjects:
ID: ISSN: 0963-6897 ; DOI: 10.3727/096368909788809839
Link: http://search.proquest.com/docview/67509643/?pq-origsite=primo
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: proquest67509643
title: A novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.
format: Article
creator:
  • Zou, Xiao Hui
  • Cai, Hong Xin
  • Yin, Zi
  • Chen, Xiao
  • Jiang, Yang Zi
  • Hu, Hu
  • Ouyang, Hong Wei
subjects:
  • Animals–Cytology
  • Bone Regeneration–Methods
  • Bone Transplantation–Methods
  • Cells, Cultured–Methods
  • Humans–Methods
  • Male–Methods
  • Mesenchymal Stem Cell Transplantation–Methods
  • Mesenchymal Stem Cells–Methods
  • Mice–Methods
  • Mice, Nude–Methods
  • Osteogenesis–Methods
  • Rabbits–Methods
  • Tissue Engineering–Methods
  • Transplantation, Homologous–Methods
ispartof: Cell transplantation, 2009, Vol.18(4), pp.433-441
description: Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cultured to form a cell sheet. The MSC sheet was then wrapped onto structural allografts. The assembled structures were cultured in vitro to evaluate the differentiation potential of MSC sheet. The assembled structures were implanted subcutaneously into nude mice as well as into the segmental radius defect of rabbits to investigate the efficiency of MSC sheets to repopulate allografts for bone repair. MSC sheets, upon assembling on bone grafts, showed similar differentiation properties to the in situ periosteum in vitro. After implantation the MSC sheets accelerated the repopulation of bone grafts in nude mice. Moreover, MSC sheets induced thicker cortical bone formation and more efficient graft-to-bone end fusion at the segmental bone defects in rabbits. This study thus presented a novel, more efficient, and practical strategy for large weight-bearing bone reconstruction by using MSC sheets to deliver large number of MSCs to repopulate the bone allografts.
language: eng
source:
identifier: ISSN: 0963-6897 ; DOI: 10.3727/096368909788809839
fulltext: fulltext
issn:
  • 09636897
  • 0963-6897
url: Link


@attributes
ID466749417
RANK0.07
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
LOCALfalse
PrimoNMBib
record
control
sourcerecordid67509643
sourceidproquest
recordidTN_proquest67509643
sourcesystemPC
pqid67509643
ingidcog/ct/2009/00000018/00000004/art00005
display
typearticle
titleA novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.
creatorZou, Xiao Hui ; Cai, Hong Xin ; Yin, Zi ; Chen, Xiao ; Jiang, Yang Zi ; Hu, Hu ; Ouyang, Hong Wei
contributorZou, Xiao Hui (correspondence author) ; Zou, Xiao Hui (record owner)
ispartofCell transplantation, 2009, Vol.18(4), pp.433-441
identifierISSN: 0963-6897 ; DOI: 10.3727/096368909788809839
subjectAnimals–Cytology ; Bone Regeneration–Methods ; Bone Transplantation–Methods ; Cells, Cultured–Methods ; Humans–Methods ; Male–Methods ; Mesenchymal Stem Cell Transplantation–Methods ; Mesenchymal Stem Cells–Methods ; Mice–Methods ; Mice, Nude–Methods ; Osteogenesis–Methods ; Rabbits–Methods ; Tissue Engineering–Methods ; Transplantation, Homologous–Methods
languageeng
source
descriptionMesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cultured to form a cell sheet. The MSC sheet was then wrapped onto structural allografts. The assembled structures were cultured in vitro to evaluate the differentiation potential of MSC sheet. The assembled structures were implanted subcutaneously into nude mice as well as into the segmental radius defect of rabbits to investigate the efficiency of MSC sheets to repopulate allografts for bone repair. MSC sheets, upon assembling on bone grafts, showed similar differentiation properties to the in situ periosteum in vitro. After implantation the MSC sheets accelerated the repopulation of bone grafts in nude mice. Moreover, MSC sheets induced thicker cortical bone formation and more efficient graft-to-bone end fusion at the segmental bone defects in rabbits. This study thus presented a novel, more efficient, and practical strategy for large weight-bearing bone reconstruction by using MSC sheets to deliver large number of MSCs to repopulate the bone allografts.
version6
lds50peer_reviewed
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
backlink$$Uhttp://search.proquest.com/docview/67509643/?pq-origsite=primo$$EView_record_in_ProQuest_(subscribers_only)
search
creatorcontrib
0Zou, Xiao Hui
1Cai, Hong Xin
2Yin, Zi
3Chen, Xiao
4Jiang, Yang Zi
5Hu, Hu
6Ouyang, Hong Wei
titleA novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.
subject
0Animals–Cytology
1Bone Regeneration–Methods
2Bone Transplantation–Methods
3Cells, Cultured–Methods
4Humans–Methods
5Male–Methods
6Mesenchymal Stem Cell Transplantation–Methods
7Mesenchymal Stem Cells–Methods
8Mice–Methods
9Mice, Nude–Methods
10Osteogenesis–Methods
11Rabbits–Methods
12Tissue Engineering–Methods
13Transplantation, Homologous–Methods
general
0English
110.3727/096368909788809839
2MEDLINE (ProQuest)
3ProQuest Biological Science Collection
4ProQuest Natural Science Collection
5ProQuest SciTech Collection
6Biological Science Database
7Natural Science Collection
8SciTech Premium Collection
9Health Research Premium Collection
10Health Research Premium Collection (Alumni edition)
11Biological Science Index (ProQuest)
sourceidproquest
recordidproquest67509643
issn
009636897
10963-6897
rsrctypearticle
creationdate2009
addtitleCell transplantation
searchscope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
scope
01007527
11007944
21009130
310000004
410000038
510000050
610000120
710000159
810000238
910000253
1010000260
1110000270
1210000271
1310000302
1410000350
15proquest
lsr43
01007527false
11007944false
21009130false
310000004false
410000038false
510000050false
610000120false
710000159false
810000238false
910000253false
1010000260false
1110000270false
1210000271false
1310000302false
1410000350false
contributorZou, Xiao Hui
startdate20090101
enddate20090101
citationpf 433 pt 441 vol 18 issue 4
lsr30VSR-Enriched:[pqid, description, ingid, eissn]
sort
titleA novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.
authorZou, Xiao Hui ; Cai, Hong Xin ; Yin, Zi ; Chen, Xiao ; Jiang, Yang Zi ; Hu, Hu ; Ouyang, Hong Wei
creationdate20090101
lso0120090101
facets
frbrgroupid9194628406965707105
frbrtype5
newrecords20181218
languageeng
creationdate2009
topic
0Animals–Cytology
1Bone Regeneration–Methods
2Bone Transplantation–Methods
3Cells, Cultured–Methods
4Humans–Methods
5Male–Methods
6Mesenchymal Stem Cell Transplantation–Methods
7Mesenchymal Stem Cells–Methods
8Mice–Methods
9Mice, Nude–Methods
10Osteogenesis–Methods
11Rabbits–Methods
12Tissue Engineering–Methods
13Transplantation, Homologous–Methods
collection
0MEDLINE (ProQuest)
1ProQuest Biological Science Collection
2ProQuest Natural Science Collection
3ProQuest SciTech Collection
4Biological Science Database
5Natural Science Collection
6SciTech Premium Collection
7Health Research Premium Collection
8Health Research Premium Collection (Alumni edition)
9Biological Science Index (ProQuest)
prefilterarticles
rsrctypearticles
creatorcontrib
0Zou, Xiao Hui
1Cai, Hong Xin
2Yin, Zi
3Chen, Xiao
4Jiang, Yang Zi
5Hu, Hu
6Ouyang, Hong Wei
jtitleCell transplantation
toplevelpeer_reviewed
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
aulast
0Zou
1Cai
2Yin
3Chen
4Jiang
5Hu
6Ouyang
aufirst
0Xiao Hui
1Hong Xin
2Zi
3Xiao
4Yang Zi
5Hu
6Hong Wei
au
0Zou, Xiao Hui
1Cai, Hong Xin
2Yin, Zi
3Chen, Xiao
4Jiang, Yang Zi
5Hu, Hu
6Ouyang, Hong Wei
addauZou, Xiao Hui
atitleA novel strategy incorporated the power of mesenchymal stem cells to allografts for segmental bone tissue engineering.
jtitleCell transplantation
risdate20090101
volume18
issue4
spage433
epage441
pages433-441
issn0963-6897
formatjournal
genrearticle
ristypeJOUR
doi10.3727/096368909788809839
urlhttp://search.proquest.com/docview/67509643/
eissn15553892
date2009-01-01